Zymosan and carrageenan represent two inflammatory stimuli leading to significant neutrophilia when injected into mice. Despite several similarities between the two proinflammatory agents, the mechanisms leading to neutrophil influx into the site of stimulus injection are unclear. As demonstrated by antibody (Ab) studies directed against adhesion molecules, L-selectin was pivotal for zymosan-induced but not carrageenan-induced pleurisy. Zymosan but not carrageenan injection into the pleural cavity caused blood neutrophilia and significant release of neutrophils from the bone marrow, events that were inhibited by anti-L-selectin but not anti-Mac-1 Ab pretreatment. Pertussis toxin, known to regulate cell efflux, abrogated both zymosanand carrageenan-induced pleurisy, but only zymosan-induced neutrophil release from the bone marrow. Dexamethasone, known to inhibit pleurisy induced by either stimulus, had no effect on bone marrow neutrophil numbers. The G i/o G proteincoupled H 4 histamine receptor is highly expressed in the bone marrow and on leukocytes and plays an important role in zymosan-induced pleurisy in vivo. Zymosan-triggered neutrophil release from bone marrow was abrogated by pretreatment of mice with thioperamide, a known H 3/4 receptor antagonist, whereas H 1 and H 2 receptor antagonists had no effect. Moreover, histamine itself, when injected intravenously, led to a similar time-and dose-dependent decrease of neutrophil numbers in the bone marrow that was inhibited by thioperamide. Because the H 3 receptor is not expressed on neutrophils, these findings indicate that both H 4 and L-selectin regulate zymosaninduced neutrophil release from bone marrow and subsequent infiltration in the pleurisy model. Acute inflammation can be experimentally induced by numerous stimuli (for example, lipopolysaccharides, phorbol esters, zymosan, and carrageenan) and is orchestrated by a complex cellular and biochemical network that involves a multitude of cell types and mediators. Due to the involvement of a high number of molecular players these models are widely used to screen for the efficacy of novel anti-inflammatory drug candidates.To experimentally induce leukocyte trafficking, carrageenan (sulfated polyanionic polysaccharide) and zymosan have become two commonly used inflammatory agents (Ohishi, 1997). Carrageenan-induced pleurisy is an experimental model of acute inflammation characterized by the migration of phagocytic cells. Polymorphonuclear leukocytes are the predominant cell type infiltrating the pleural cavity within the first 12 h after carrageenan injection. Later, polymorphonuclear cells disappear and are replaced by migrating mononuclear cells, which differentiate into macrophages and dominate the reaction up to its resolution at 48 h (Tomlinson et al., 1994;Willis et al., 1996). These inflammatory cells synthesize and release various mediators of inflammation, among which the kallirein-kinin system and prostaglandins play a pivotal role. Inhibitors of cyclooxygenase, kallikreinkinin, and brady...