A possible role of oxidative stress in the switch mechanism of the cell death mode from apoptosis to necrosis – studies on ρ0 cells

Wochna, Agnieszka; Niemczyk, Edyta; Kurono, Chieko; Masaoka, Makoto; Kedzior, Jakub; Słomińska, Ewa; Lipiński, Marcin; Wakabayashi, Takashi

doi:10.1016/j.mito.2006.11.005

Cited by 34 publications

(17 citation statements)

References 22 publications

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“…To verify that CoQ deficiency was the cause of the degenerative phenotype in C. elegans, we incubated coq-1 RNAi-treated animals with CoQ 10 . We found that supplemental Coenzyme Q 10 rescued degeneration in a dose-dependent manner, with an EC 50 of 72 mg/ mL (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Coenzyme Q protects Caenorhabditis elegans GABA neurons from calcium-dependent degeneration

Earls

Hacker²,

Watson³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Mitochondria are key regulators of cell viability and provide essential functions that protect against neurodegenerative disease. To develop a model for mitochondrial-dependent neurodegeneration in Caenorhabditis elegans, we used RNA interference (RNAi) and genetic ablation to knock down expression of enzymes in the Coenzyme Q (CoQ) biosynthetic pathway. CoQ is a required component of the ATP-producing electron transport chain in mitochondria. We found that reduced levels of CoQ result in a progressive uncoordinated (Unc) phenotype that is correlated with the appearance of degenerating GABA neurons. Both the Unc and degenerative phenotypes emerge during late larval development and progress in adults. Neuron classes in motor and sensory circuits that use other neurotransmitters (dopamine, acetylcholine, glutamate, serotonin) and body muscle cells were less sensitive to CoQ depletion. Our results indicate that the mechanism of GABA neuron degeneration is calcium-dependent and requires activation of the apoptotic gene, ced-4 (Apaf-1). A molecular cascade involving mitochondrial-initiated cell death is also consistent with our finding that GABA neuron degeneration requires the mitochondrial fission gene, drp-1. We conclude that the cell selectivity and developmental progression of CoQ deficiency in C. elegans indicate that this model may be useful for delineating the role of mitochondrial dysfunction in neurodegenerative disease.cell death | disease | mitochondria | neurodegeneration | necrosis

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Section: Resultsmentioning

confidence: 99%

“…Additionally, apoptosis and necrosis share common molecular triggers, such as oxidative stress and energy depletion, two hallmarks of mitochondrial dysfunction (10,11). These findings suggest that apoptotic and necrotic pathways may represent related cell death responses and can be activated by mitochondrial impairment.…”

mentioning

confidence: 95%

Coenzyme Q protects Caenorhabditis elegans GABA neurons from calcium-dependent degeneration

Earls

Hacker²,

Watson³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Therefore, the major goal of cancer therapy is to selectively kill cancer cells without harming normal cells. There are three major ways by which a cancer cell can die: apoptosis, necrosis, and autophagy (283,285,329).…”

Section: Role Of Ros In Tumor Cell Deathmentioning

confidence: 99%

Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy

Gupta

Hevia

Patchva

et al. 2012

Antioxidants & Redox Signaling

621

464

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Significance: Extensive research during the last quarter century has revealed that reactive oxygen species (ROS) produced in the body, primarily by the mitochondria, play a major role in various cell-signaling pathways. Most risk factors associated with chronic diseases (e.g., cancer), such as stress, tobacco, environmental pollutants, radiation, viral infection, diet, and bacterial infection, interact with cells through the generation of ROS. Recent Advances: ROS, in turn, activate various transcription factors (e.g., nuclear factor kappa-light-chain-enhancer of activated B cells [NF-jB], activator protein-1, hypoxia-inducible factor-1a, and signal transducer and activator of transcription 3), resulting in the expression of proteins that control inflammation, cellular transformation, tumor cell survival, tumor cell proliferation and invasion, angiogenesis, and metastasis. Paradoxically, ROS also control the expression of various tumor suppressor genes (p53, Rb, and PTEN). Similarly, c-radiation and various chemotherapeutic agents used to treat cancer mediate their effects through the production of ROS. Interestingly, ROS have also been implicated in the chemopreventive and anti-tumor action of nutraceuticals derived from fruits, vegetables, spices, and other natural products used in traditional medicine. Critical Issues: These statements suggest both ''upside'' (cancer-suppressing) and ''downside'' (cancer-promoting) actions of the ROS. Thus, similar to tumor necrosis factor-a, inflammation, and NF-jB, ROS act as a double-edged sword. This paradox provides a great challenge for researchers whose aim is to exploit ROS stress for the development of cancer therapies. Future Directions: The various mechanisms by which ROS mediate paradoxical effects are discussed in this article. The outstanding questions and future directions raised by our current understanding are discussed. Antioxid. Redox Signal. 16, 1295Signal. 16, -1322

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“…Eukaryotic cells typically respond to radiation by activating the DNA repair pathways and cell cycle checkpoints, followed by either full biological recovery or cell death (Ozben, 2007) (Figure 1). Radiation-induced ROS production can lead to cell death through several mechanisms including apoptosis, necrosis and autophagy (Ozben, 2007;Wochna et al, 2007;Azad et al, 2009).…”

Section: Free Radicals and Cell Deathmentioning

confidence: 99%

“…In many cases, apoptosis and necrosis may occur sequentially or simultaneously within the same tissue due to irradiation. Through a series of elegant studies, Wochna et al (2007) hypothesised that the switch from apoptotic to necrotic cell death involves not only a diminution in cellular adenosine triphosphate (ATP) during cellular dysfunction, but also an explosion of intracellular ROS.…”

Section: Free Radicals and Cell Deathmentioning

confidence: 99%

Herbal Remedies for Combating Irradiation: a Green Anti-irradiation Approach

Lachumy¹,

Oon²,

Deivanai³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Plants play important roles in human life not only as suppliers of oxygen but also as a fundamental resource to sustain the human race on this earthly plane. Plants also play a major role in our nutrition by converting energy from the sun during photosynthesis. In addition, plants have been used extensively in traditional medicine since time immemorial. Information in the biomedical literature has indicated that many natural herbs have been investigated for their efficacy against lethal irradiation. Pharmacological studies by various groups of investigators have shown that natural herbs possess significant radioprotective activity. In view of the immense medicinal importance of natural product based radioprotective agents, this review aims at compiling all currently available information on radioprotective agents from medicinal plants and herbs, especially the evaluation methods and mechanisms of action. In this review we particularly emphasize on ethnomedicinal uses, botany, phytochemistry, mechanisms of action and toxicology. We also describe modern techniques for evaluating herbal samples as radioprotective agents. The usage of herbal remedies for combating lethal irradiation is a green antiirradiation approach for the betterment of human beings without high cost, side effects and toxicity.

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A possible role of oxidative stress in the switch mechanism of the cell death mode from apoptosis to necrosis – studies on ρ0 cells

Cited by 34 publications

References 22 publications

Coenzyme Q protects Caenorhabditis elegans GABA neurons from calcium-dependent degeneration

Coenzyme Q protects Caenorhabditis elegans GABA neurons from calcium-dependent degeneration

Upsides and Downsides of Reactive Oxygen Species for Cancer: The Roles of Reactive Oxygen Species in Tumorigenesis, Prevention, and Therapy

Herbal Remedies for Combating Irradiation: a Green Anti-irradiation Approach

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