A possible new class of ribonucleotide reductase from Methanobacterium thermoautotrophicum

Sze, I. S.-Y.; McFarlan, Sara C.; Spormann, Alfred M.; Hogenkamp, Harry P. C.; Follmann, Hartmut

doi:10.1016/0006-291x(92)90705-p

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…Recent studies have revealed that several key metabolic intermediates take part in phosphate and purine metabolism [134, 135]. A 5-phosphoribosyl-5-amino-4-imidazolecarboxamide (AICAR) and succinyl-AICAR belong to these regulatory molecules [136].…”

Section: Phosphate Metabolismmentioning

confidence: 99%

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

et al. 2011

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Acid phosphatases of budding yeast have been studied for more than forty years. This paper covers biochemical characteristics of acid phosphatases and different aspects in expression regulation of eukaryotic genes, which were researched using acid phosphatases model. A special focus is devoted to cyclin-dependent kinase Pho85p, a negative transcriptional regulator, and its role in maintaining mitochondrial genome stability and to pleiotropic effects of pho85 mutations.

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Section: Phosphate Metabolismmentioning

confidence: 99%

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

et al. 2011

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“…This class has a di‐ferric heme group but also requires oxygen and manganese. The presence of this class of ribonucleotide reductase has been demonstrated in the Firmicute B. subtilis and the Actinobacteria Corynebacterium glutamicum and ammoniagenes and Propionibacterium shermanii [164–166] with a possible example in the Archaea in Methanobacterium thermoautotrophicum [167].…”

Section: Biochemistry Of Manganesementioning

confidence: 99%

Emerging themes in manganese transport, biochemistry and pathogenesis in bacteria

2003

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Though an essential trace element, manganese is generally accorded little importance in biology other than as a cofactor for some free radical detoxifying enzymes and in the photosynthetic photosystem II. Only a handful of other Mn2+-dependent enzymes are known. Recent data, primarily in bacteria, suggest that Mn2+-dependent processes may have significantly greater physiological importance. Two major classes of prokaryotic Mn2+ uptake systems have now been described, one homologous to eukaryotic Nramp transporters and one a member of the ABC-type ATPase superfamily. Each is highly selective for Mn2+ over Fe2+ or other transition metal divalent cations, and each can accumulate millimolar amounts of intracellular Mn2+ even when environmental Mn2+ is scarce. In Salmonella enterica serovar Typhimurium, simultaneous mutation of both types of transporter results in avirulence, implying that one or more Mn2+-dependent enzymes is essential for pathogenesis. This review summarizes current literature on Mn2+ transport, primarily in the Bacteria but with relevant comparisons to the Archaea and Eukaryota. Mn2+-dependent enzymes are then discussed along with some speculations as to their role(s) in cellular physiology, again primarily in Bacteria. It is of particular interest that most of the enzymes which interconvert phosphoglycerate, pyruvate, and oxaloacetate intermediates are either strictly Mn2+-dependent or highly stimulated by Mn2+. This suggests that Mn2+ may play an important role in central carbon metabolism. Further studies will be required, however, to determine whether these or other actions of Mn2+ within the cell are the relevant factors in pathogenesis.

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“…In particular, fragment F09 showed a high level of sequence similarity to an anaerobic ribonucleotide triphosphate reductase from Methanothermobacter thermautotrophicus . The Archaeal enzyme, involved in the reduction of nucleotide diphosphates to nucleotide triphosphates, has distinguishing characteristics that set it apart from its Bacterial counterparts such as susceptibilities to S‐adenosylmethionine, oxygen, and azide, and an independence from coenzyme B12, manganese, and iron (Sprengel and Follmann, 1981; Sze et al ., 1992). Moreover, it has been suggested that the Archaeal ribonucleotide reductase may have a different activation mechanism than Bacterial ribonucleotide reductases (Sze et al ., 1992).…”

Section: Resultsmentioning

confidence: 99%

Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model

Galbraith

Antonopoulos

White

2004

Environmental Microbiology

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Molecular techniques previously used for genome comparisons of closely related bacterial species could prove extremely valuable for comparisons of complex microbial communities, or metagenomes. Our study aimed to determine the breadth and value of suppressive subtractive hybridization (SSH) in a pilot-scale analysis of metagenomic DNA from communities of microorganisms in the rumen. Suppressive subtractive hybridization was performed using total genomic DNA isolated from rumen fluid samples of two hay-fed steers, arbitrarily designated as tester or driver. Ninety-six subtraction DNA fragments from the tester metagenome were amplified, cloned and the DNA sequences were determined. Verification of the isolation of DNA fragments unique to the tester metagenome was accomplished through dot blot and Southern blot hybridizations. Tester-specific SSH fragments were found in 95 of 96 randomly selected clones. DNA sequences of subtraction fragments were analysed by computer assisted DNA and amino acid comparisons. Putative translations of 26 (32.1%) subtractive hybridization fragments exhibited significant similarity to Bacterial proteins, whereas 15 (18.5%) distinctive subtracted fragments had significant similarity to proteins from Archaea. The remainder of the subtractive hybridization fragments displayed no similarity to GenBank sequences. This metagenomic approach has exposed an unexpectedly large difference in Archaeal community structure between the rumen microbial populations of two steers fed identical diets and housed together. 16S rRNA dot blot hybridizations revealed similar proportions of Bacteria and Archaea in both rumen samples and suggest that the differences uncovered by SSH are the result of varying community structural composition. Our study demonstrates a novel approach to comparative analyses of environmental microbial communities through the use of SSH.

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A possible new class of ribonucleotide reductase from Methanobacterium thermoautotrophicum

Cited by 11 publications

References 18 publications

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

Emerging themes in manganese transport, biochemistry and pathogenesis in bacteria

Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model

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