A possible mechanism for visible light‐induced wound healing

Lipovsky, Anat; Nitzan, Yeshayahu; Lubart, Rachel

doi:10.1002/lsm.20668

Cited by 55 publications

(40 citation statements)

References 39 publications

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“…However, neither wavelength was effective on the anaerobe P. acne. 14 More recently, Lipovsky et al 18 demonstrated that high-intensity broad-spectrum polychromatic light with wavelengths in the range of 400-1000 nm kills bacteria in infected diabetic ulcers.…”

Section: Introductionmentioning

confidence: 98%

Blue 470-nm Light Kills Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro

Enwemeka

Williams

Enwemeka

et al. 2009

Photomedicine and Laser Surgery

171

128

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Section: Introductionmentioning

confidence: 98%

Blue 470-nm Light Kills Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro

Enwemeka

Williams

Enwemeka

et al. 2009

Photomedicine and Laser Surgery

171

128

View full text Add to dashboard Cite

show abstract

“…In another study, Lipovsky et al [22] found that following illumination, colonies of Escherichia coli, Staphylococcus aureus and Staphylococcus marcescens were reduced by 62, 83 and 56% respectively. In our series, six patients with chronic sinuses (9-44 months), intractable to surgical and conservative measures, healed within 4 months (range: 1.5-4 months, mean: 2.17 months) of starting laser depilation.…”

Section: Discussionmentioning

confidence: 96%

Recurrent pilonidal sinus disease: Do lasers have the answer?

Kok

Osmani

Odeke

et al. 2011

Medical Laser Application

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“…Recently there were several publications on the bactericidal effect of visible light, most of them claiming that blue part of the spectrum (400 nm-500 nm) is responsible for killing various pathogens [1][2][3][4][5] . The phototoxic effect of blue light was suggested to be a result of light-induced reactive oxygen species (ROS) formation by endogenous bacterial photosensitizers which mostly absorb light in the blue region 4,6,7 .…”

Section: Introductionmentioning

confidence: 99%

The Synergistic Effect of Visible Light and Gentamycin on <em>Pseudomona aeruginosa</em> Microorganisms

Reznick

Banin

Lipovsky³

et al. 2013

JoVE

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Recently there were several publications on the bactericidal effect of visible light, most of them claiming that blue part of the spectrum (400 nm-500 nm) is responsible for killing various pathogens [1][2][3][4][5] . The phototoxic effect of blue light was suggested to be a result of light-induced reactive oxygen species (ROS) formation by endogenous bacterial photosensitizers which mostly absorb light in the blue region 4,6,7 . There are also reports of biocidal effect of red and near infra red 8 as well as green light 9 .In the present study, we developed a method that allowed us to characterize the effect of high power green (wavelength of 532 nm) continuous (CW) and pulsed Q-switched (Q-S) light on Pseudomonas aeruginosa. Using this method we also studied the effect of green light combined with antibiotic treatment (gentamycin) on the bacteria viability. P. aeruginosa is a common noscomial opportunistic pathogen causing various diseases. The strain is fairly resistant to various antibiotics and contains many predicted AcrB/Mex-type RND multidrug efflux systems 10 .The method utilized free-living stationary phase Gram-negative bacteria (P. aeruginosa strain PAO1), grown in Luria Broth (LB) medium exposed to Q-switched and/or CW lasers with and without the addition of the antibiotic gentamycin. Cell viability was determined at different time points.The obtained results showed that laser treatment alone did not reduce cell viability compared to untreated control and that gentamycin treatment alone only resulted in a 0.5 log reduction in the viable count for P. aeruginosa. The combined laser and gentamycin treatment, however, resulted in a synergistic effect and the viability of P. aeruginosa was reduced by 8 log's.The proposed method can further be implemented via the development of catheter like device capable of injecting an antibiotic solution into the infected organ while simultaneously illuminating the area with light. Video LinkThe video component of this article can be found at http://www.jove.com/video/4370/ Protocol 1. Bacterial Culture 1. Gram-negative P. aeruginosa strain PAO1 were grown in Luria Broth (LB) at 37 °C for 18 hr. 2. The culture of cells was then centrifuged at 7,500 rpm (rounds per minute) for 5 min and supernatant was removed. 3. The bacteria were resuspended in 10% LB and re-grown for another 2 hr to allow the culture to reenter stationary phase. 4. The bacteria suspension was then divided into two groups: in the first group (2 tubes) no antibiotic were added, in the second group we added the gentamycin antibiotic (50 μg/ml). Determination of Colony-forming Units (CFU)1. To determine cell viability 20 μl samples were taken from the experiment approximately every 2 hr within the time frame of 24 hr. Serial dilution of the samples were made and plated on LB agar plates and incubated overnight at 37 °C.

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A possible mechanism for visible light‐induced wound healing

Abstract: Visible light at high intensity can kill bacteria in infected wounds. Thus, illumination of infected wounds with intense visible light, prior to low intensity illumination for stimulating wound closure, may reduce infection and promote healing.

Cited by 55 publications

References 39 publications

Blue 470-nm Light Kills Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro

Blue 470-nm Light Kills Methicillin-Resistant Staphylococcus aureus (MRSA) in Vitro

Recurrent pilonidal sinus disease: Do lasers have the answer?

The Synergistic Effect of Visible Light and Gentamycin on <em>Pseudomona aeruginosa</em> Microorganisms

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