1977
DOI: 10.4049/jimmunol.119.2.576
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A Possible Difference in the Heterogeneity of the Heavy and Light Chains from a Rabbit Anti-P-Azobenzoate Antibody Preparation

Abstract: CNBr cleavage of rabbit heavy (H) chains leads to the formation of a fragment, C-1, which consists of the N-terminal half of the H chain. Fragment C-1 is cleaved at methionyl residues but held together by intrachain S-S bonds so that smaller fragments can be liberated by total reduction and alkylation. In the case of the C-1 fragment from an anti-p-azobenzoate antibody preparation, which has a light (L) chain of markedly restricted heterogeneity, total reduction and alkylation liberated seven major fragments i… Show more

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