A possible biological role of boar sperm diaphorase investigated by a specific monoclonal antibody

The effect of three nitric oxide (NO) synthase inhibitors, L-NG-nitro-arginine (NO2Arg), NG-Nitro-L-arginine methyl ester (L-NAME) and aminoguanidine, on in vitro fertilization in the mouse was examined. Mouse epididymal spermatozoa were capacitated in a medium with or without NO synthase inhibitors. Oocytes were inseminated and the percentage of oocytes with two pronuclei was scored after an 8-h incubation. NO2Arg and L-NAME, but not aminoguanidine, inhibited fertilization. L-NAME inhibited fertilization in a dose-dependent manner, and its effects were stereospecific. The inhibitory effect was neutralized by L-arginine but not by D-arginine. Moreover, D-NAME did not inhibit fertilization. The results suggest that NO synthase activity (presumably of the constitutive type is necessary for spermatozoa to display their full fertilizing ability.

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A possible biological role of boar sperm diaphorase investigated by a specific monoclonal antibody

Cited by 4 publications

References 24 publications

Changes in immunochemical localisation of acrosomal and sperm proteins in boar spermatozoa during capacitation and induced acrosome reaction

Changes in immunochemical localisation of acrosomal and sperm proteins in boar spermatozoa during capacitation and induced acrosome reaction

Identification and isolation of boar sperm specific antigens with potential role in sperm–egg interaction

Effects of nitric oxide synthase inhibitors on the outcome of in vitro fertilization in the mouse

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