A portable spinning disc for complete blood count (CBC)

Agarwal, Rahul; Sarkar, Arnab; Bhowmik, Arka; Mukherjee, Devdeep; Chakraborty, Suman

doi:10.1016/j.bios.2019.111935

Cited by 23 publications

(18 citation statements)

References 35 publications

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“…In the gold standard practice, blood hemoglobin is transformed into cyanmethemoglobin using Drabkin's solution for spectrophotometric analysis in hematology analyser 6,7 . Significant efforts have been put forward towards developing low-cost, userfriendly, POC device as a viable alternative to the bulky sophisticated instruments used in conventional laboratories for hematological analysis [8][9][10][11][12][13][14][15][16][17][18][19][20] . In this regard, several techniques such as colorimetric detection using 2,7-diaminofluorene, electrochemical detection, luminescence assays, carbon-dot based fluorescence assays etc.…”

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confidence: 99%

Smartphone-Enabled Paper-Based Hemoglobin Sensor for Extreme Point-of-Care Diagnostics

et al. 2021

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We report a simple, affordable (~ 0.02 US $/test), rapid (within 5 minutes) and quantitative paper-based sensor integrated with smartphone application for on-spot detection of hemoglobin (Hgb) concentration using approximately 10µl of finger-pricked blood. Quantitative analytical colorimetry is achieved via android-based application (Sens-Hb); integrating key operational steps of image acquisition, real-time analysis and result dissemination. Further, feedback from machine learning algorithm for adaptation of calibration data offers consistent dynamic improvement for precise predictions of the test results. Our study reveals a successful deployment of the extreme point-of-care (EPOC) test in rural settings where no infrastructural facilities for diagnostics are available. The Hgb test device is validated both in the controlled laboratory environment (n = 200) and on the field experiments (n = 142) executed in four different Indian villages. Validation results are wellcorrelated with the pathological gold standard results (r = 0.9583) with high sensitivity and specificity for healthy (n=136) (>11 g/dL) (specificity: 97.2%), mild anemic (n = 55) (<11 g/dL) (sensitivity: 87.5%, specificity: 100%) and severe anemic (n = 9) (<7 g/d L) (sensitivity: 100%, specificity: 100 %) samples. Results from field trials reveal that only below 5% cases of the results are interpreted erroneously by classifying mild anemic patients as healthy ones. On-field deployment has unveiled the test-kit to be extremely user-friendly that can be handled by minimally trained frontline workers for catering the needs of the under-served communities.

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Smartphone-Enabled Paper-Based Hemoglobin Sensor for Extreme Point-of-Care Diagnostics

et al. 2021

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“…A further approach for analyzing blood is performed on a rotating disc. It utilized density gradient centrifugation for complete blood counting and achieved an accuracy of >95% as compared to an automated hematology analyzer …”

Section: Selected Applications In Bioanalysis and Diagnosticsmentioning

confidence: 99%

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

et al. 2020

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“…Recently they created an innovative paper alternative to commercial centrifuges, separating red blood cells from plasma to provide a pure enough sample for malaria identification [ 137 ]. A similar approach has been taken to create an alternative to expensive automated haematology analysers [ 138 ]. Figure 12 demonstrates the operating principle of this device, as red blood cells have a higher density than blood plasma, they are pushed to the outside of the disk, white blood cells in contrast, have a lower density and move towards the centre of the disk.…”

Section: Mechanical Biosensorsmentioning

confidence: 99%

“…Figure 12 demonstrates the operating principle of this device, as red blood cells have a higher density than blood plasma, they are pushed to the outside of the disk, white blood cells in contrast, have a lower density and move towards the centre of the disk. After separating blood into its components, automated counting can then by accomplished using Image J [ 138 ]. Another solution to a lack of equipment is to adapt equipment already available.…”

Section: Mechanical Biosensorsmentioning

confidence: 99%

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Biosensors Based on Mechanical and Electrical Detection Techniques

Chalklen

Jing

Kar‐Narayan

2020

Sensors

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Biosensors are powerful analytical tools for biology and biomedicine, with applications ranging from drug discovery to medical diagnostics, food safety, and agricultural and environmental monitoring. Typically, biological recognition receptors, such as enzymes, antibodies, and nucleic acids, are immobilized on a surface, and used to interact with one or more specific analytes to produce a physical or chemical change, which can be captured and converted to an optical or electrical signal by a transducer. However, many existing biosensing methods rely on chemical, electrochemical and optical methods of identification and detection of specific targets, and are often: complex, expensive, time consuming, suffer from a lack of portability, or may require centralised testing by qualified personnel. Given the general dependence of most optical and electrochemical techniques on labelling molecules, this review will instead focus on mechanical and electrical detection techniques that can provide information on a broad range of species without the requirement of labelling. These techniques are often able to provide data in real time, with good temporal sensitivity. This review will cover the advances in the development of mechanical and electrical biosensors, highlighting the challenges and opportunities therein.

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A portable spinning disc for complete blood count (CBC)

Cited by 23 publications

References 35 publications

Smartphone-Enabled Paper-Based Hemoglobin Sensor for Extreme Point-of-Care Diagnostics

Smartphone-Enabled Paper-Based Hemoglobin Sensor for Extreme Point-of-Care Diagnostics

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Biosensors Based on Mechanical and Electrical Detection Techniques

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