A portable silicon photodiode luminometer

Marks, Kim; Killeen, Paul R.; Goundry, J.; Gibbons, John; Bunce, Roger

doi:10.1002/bio.1170010305

Cited by 12 publications

(4 citation statements)

References 4 publications

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“…Achieving high sensitivity with a PIN photodiode requires excessive external gain, leading to high sensitivity to external electric and magnetic fields. For example, a PIN photodiode-based luminometer device described in the literature [ 17 ] ultimately had its sensitivity limited by spurious electrostatic charge distributions affecting baseline dark current.…”

Section: Design Of the Luminometermentioning

confidence: 99%

“…Beyond commercial luminometers, the landscape of low-cost, portable luminometers reported in the academic literature cannot match the LOD performance standards established by PMT-based commercial instruments ( [14][15][16][17][18][19][20][21][22] and S1 Table ). In general, we found that the limit of detection of non-PMT devices in the academic literature ranges 100-to 100,000-fold inferior to commercial PMT-based readers.…”

Section: Introductionmentioning

confidence: 99%

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A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel,

Elledge,

Wiener

et al. 2024

PLOS Glob Public Health

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Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase. Using these devices, we performed two pilot cross-sectional serology studies to assess sars-cov-2 antibody levels: a cohort in the United States, as well as a field study in Bangladesh. Results from both studies were consistent with previous work and demonstrate the device’s suitability for distributed applications in global health.

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Section: Design Of the Luminometermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel,

Elledge,

Wiener

et al. 2024

PLOS Glob Public Health

View full text Add to dashboard Cite

show abstract

“…Beyond commercial luminometers, the landscape of low-cost, portable luminometers reported in the academic literature cannot match the LOD performance standards established by PMT-based commercial instruments ( [14][15][16][17][18][19][20][21][22] and Supplementary Table 1). In general, we found that the limit of detection of non-PMT devices in the academic literature ranges 100-to 100,000-fold inferior to commercial PMT-based readers.…”

Section: Introductionmentioning

confidence: 99%

A handheld luminometer with sub-attomole limit of detection for distributed applications in global health

Lebel

Elledge²,

Wiener

et al. 2023

Preprint

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Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase, or approximately 1fW of radiant optical power. Using these devices, we performed split luciferase serology studies to monitor sars-cov-2 antibodies in a cohort in the United States, as well as a field study in Bangladesh.

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“…Luminometers are central to all luminescence studies and in the past, journals have published extensive surveys of luminometers . In addition, the literature contains numerous reviews and evaluations of luminometers and descriptions of luminometers based on charge‐coupled detectors (CCD) invented in 1969 , low cost detectors (e.g., photographic film, silicon photodiodes) , single photon counting and luminometers designed for specific types of reaction vessels such as microplates or for direct injection of reagents for luminescence reactions with rapid emission kinetics .…”

mentioning

confidence: 99%