A portable personal glucose meter method for enzyme activity detection and inhibitory activity evaluation based on alkaline phosphatase-mediated reaction

Zhang, Hao; Chen, Guoying; Qian, Zhengming; Li, Wenjia; Li, Chunhong; Y, Hu; Yang, Feng‐Qing

doi:10.1007/s00216-021-03187-w

Cited by 14 publications

(8 citation statements)

References 28 publications

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“…Through this process, several 6-membered cyclic products were obtained in moderate to excellent yields and excellent enantiomeric ratios (er). Several known bioactive motifs were synthesized, such as theophylline and purine derivatives 169-175 (Scheme 55B) [261][262][263]. The authors also proposed a plausible mechanism (Scheme 55C).…”

Section: Nickel-catalyzed C-h Activationmentioning

confidence: 99%

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

Carvalho¹,

Miranda²,

Nunes³

et al. 2021

Beilstein J. Org. Chem.

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Several valuable biologically active molecules can be obtained through C–H activation processes. However, the use of expensive and not readily accessible catalysts complicates the process of pharmacological application of these compounds. A plausible way to overcome this issue is developing and using cheaper, more accessible, and equally effective catalysts. First-row transition (3d) metals have shown to be important catalysts in this matter. This review summarizes the use of 3d metal catalysts in C–H activation processes to obtain potentially (or proved) biologically active compounds.

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Section: Nickel-catalyzed C-h Activationmentioning

confidence: 99%

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

Carvalho¹,

Miranda²,

Nunes³

et al. 2021

Beilstein J. Org. Chem.

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“…The PGM depends on the amount of current generated by the reaction between glucose and glucose oxidase or dehydrogenase in the test strip to reflect the concentration of glucose in the blood. Based on the testing principle of PGM, many strategies have been investigated to transform PGM into a common meter for analyzing non-glucose targets such as heavy metal ions, enzymes, mycotoxins, pathogens, and pesticides [ 24 , 25 , 26 , 27 , 28 , 29 ]. Most of these strategies require combination with glucose-loaded materials or glucose-related enzymes to produce glucose, modified glucose test strips, or join with other technical equipment.…”

Section: Introductionmentioning

confidence: 99%

A Catechol-Meter Based on Conventional Personal Glucose Meter for Portable Detection of Tyrosinase and Sodium Benzoate

et al. 2022

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In this study, the personal glucose meter (PGM) was first used as a fast and user-friendly meter for analyzing catechol (CA) based on the reduction of the mediator K3[Fe(CN)6] to K4[Fe(CN)6] in the glucose test strip. Then, an easy, low-cost, and convenient PGM-based method for detecting tyrosinase (TYR) activity and sodium benzoate (SBA) was developed on the basis of the TYR-catalyzed reaction. In this method, CA is oxidized to form o-benzoquinone by TYR, thereby reducing the residual amount of CA and the PGM readout. On the other hand, SBA can inhibit the oxidation of CA catalyzed by TYR and increase the residual amount of CA after the enzymatic reaction. Therefore, the activity of TYR is proportional to the difference in the PGM readout of CA, and the concentration of SBA is positively correlated with the residual amount of CA. After the relevant experimental conditions were systematically optimized, the proposed PGM-based method for the detection of TYR and SBA was successfully validated. The liner ranges are 1.0–103.3 U/mL and 6.25–1000 ppm, and the quantification limits are 1.0 U/mL and 6.25 ppm for TYR and SBA, respectively. Moreover, the spiked recovery tests in normal human serum and carbonate beverages (i.e., Cola, Sprite, and Fanta) were performed, and the recoveries (91.6–106.8%) further confirm the applicability of the PGM-based method in real sample analysis.

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“…As one of the most successful and effective tools of POCT, personal glucose meter (PGM) is widely used for its less analysis time, simple operation, portability, and low cost [22]. Moreover, many methods based on the PGM for measuring analytes other than glucose have been reported, including DNA [23], enzyme activity and their inhibitors [24][25][26], and heavy metals [27]. For example, a green PGM method was developed for direct determination of hydrogen peroxide and hypochlorite by us [28].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Portable Personal Glucose Meter Method Combined with Molecular Docking for Screening of Lipase Inhibitors

Zhang

Yang

Gao

2022

Evidence-Based Complementary and Alternative Medicine

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With the increase of obesity incidence, the development of antiobesity drugs has aroused extensive interest. In this study, a simple and portable personal glucose meter (PGM) method based on the lipase-mediated reaction combined with molecular docking was developed for the screening of lipase inhibitors. Lipase can catalyse the hydrolysis of 4-acetamidophenyl acetate to form acetaminophen, which can directly trigger the reduction of K3[Fe(CN)6] to K4[Fe(CN)6] in the glucose test strips and generate an electrical signal that can be detected by the PGM. When lipase inhibitors exist, the yield of acetaminophen will be reduced and results in a corresponding decrease of the PGM signal. Therefore, the activity of lipase can be measured by the PGM. After optimization of the experimental conditions, the inhibitory activity of fourteen small-molecule compounds and fifteen natural product extracts on lipase were evaluated by the developed PGM method. The results indicate that tannic acid, (-)-epigallocatechin gallate, (-)-epigallocatechin, (-)-epicatechin gallate, and epicatechin have good inhibitory effect on lipase (% of inhibition higher than 40.0%). Besides, the natural product extracts of Galla Chinensis, lemon, and Rhei Radix et Rhizoma have a good inhibitory effect on lipase with % of inhibition of (97.5 ± 0.6)%, (88.1 ± 0.7)%, and (79.1 ± 1.6)%, respectively. Finally, the binding sites and modes of six small-molecule compounds on lipase were investigated by the molecular docking study. The results show that the developed PGM method is an effective approach for the discovery of potential lipase inhibitors.

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A portable personal glucose meter method for enzyme activity detection and inhibitory activity evaluation based on alkaline phosphatase-mediated reaction

Cited by 14 publications

References 28 publications

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

On the application of 3d metals for C–H activation toward bioactive compounds: The key step for the synthesis of silver bullets

A Catechol-Meter Based on Conventional Personal Glucose Meter for Portable Detection of Tyrosinase and Sodium Benzoate

A Simple and Portable Personal Glucose Meter Method Combined with Molecular Docking for Screening of Lipase Inhibitors

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