A portable optical waveguide resonance light-scattering scanner for microarray detection

Xing, Xuefeng; Liu, Wanyao; Li, Tao; Xing, Shu; Fu, Xueqi; Wu, Dajun; Liu, Dianjun

doi:10.1039/c5an01839k

Cited by 6 publications

(4 citation statements)

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“…The recovered ssDNAs were measured by a well-established DNA microarray-based RLS assay. 52,53 The T M and/or T W standard solutions were employed to calibrate the RLS assay response to the corresponding ssDNAs concentrations. The recovery yield of the ssDNA (Y ssDNA ) is calculated by the following equation:…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Under the optimized experimental conditions, the DNA recovery efficiency was evaluated using T M - and/or T W (wild type of T M )-spiked solutions. The recovered ssDNAs were measured by a well-established DNA microarray-based RLS assay. , The T M and/or T W standard solutions were employed to calibrate the RLS assay response to the corresponding ssDNAs concentrations. The recovery yield of the ssDNA ( Y ssDNA ) is calculated by the following equation: Y ssDNA = ( C 1 V 1 )/ ( C 0 V 0 ) × 100%; herein, C 0 and V 0 are the concentration and volume of the original ssDNA solution, while C 1 and V 1 are the concentration and volume of the recovered ssDNA solution, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma

et al. 2020

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A facile method has been developed for the rapid and efficient enrichment of DNAs from different media including synthetic single-strand DNAs (ssDNAs) from buffer solutions and cell-free DNAs (cfDNAs) from blood plasma through electric fielddriven adsorption and desorption of DNAs by a polyacrylamide/ phytic acid/polydopamine (PAAM/PA/PDA) hydrogel. The asprepared PAAM/PA/PDA hydrogel possesses regular porosity with a large surface area, strong electric field responsiveness/good conductivity, and a rich aromatic structure, which can be used as an ideal adsorbent for DNA enrichment under a positive electric field. The enriched DNAs can be released efficiently when the positive electric field is converted to a negative electric field. The PAAM/PA/PDA hydrogel-based electrochemical method enables the completion of the process of DNA adsorption and release within 5 min and exhibits reasonable enrichment efficiencies and recovery rates of various DNAs. For instance, the high enrichment sensitivity (0.1 pmol L −1 ) together with the excellent recovery (>75%) of an ssDNA with 78 nucleotides is obtained. Combined with the PCR amplification technique, the practicability of the as-proposed method is demonstrated by the screening of circulating tumor DNAs (ctDNAs) with a BRAF V600E mutation in cfDNAs from the blood plasma samples of patients with papillary thyroid cancer or thyroid nodule and random patients from a clinical laboratory.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma

et al. 2020

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“…In fact, nanoscale objects without any fluorescence labelling (e.g. gold NPs, viruses etc) are possible to detect with high temporal and axial resolution using darkfield microscopy [20,[34][35][36][37]. In this regard, evanescent scattering, which has a much higher signal yield than fluorescence can be used to detect single nanometer objects with high precision.…”

Section: Visualization Of Non-fluorescent Particlesmentioning

confidence: 99%

Compact 3D printed module for fluorescence and label-free imaging using evanescent excitation

Pandey

Gupta

Elangovan

2017

Methods Appl. Fluoresc.

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Total internal reflection fluorescence (TIRF) microscopy is widely used for selective excitation and high-resolution imaging of fluorophores, and more recently label-free nanosized objects, with high vertical confinement near a liquid-solid interface. Traditionally, high numerical aperture objectives (>1.4) are used to simultaneously generate evanescent waves and collect fluorescence emission signals which limits their use to small area imaging (<0.1 mm). Objective-based TIRFs are also expensive as they require dichroic mirrors and efficient notch filters to prevent specular reflection within the objective lenses. We have developed a compact 3D module called cTIRF that can generate evanescent waves in microscope glass slides via a planar waveguide illumination. The module can be attached as a fixture to any existing optical microscope, converting it into a TIRF and enabling high signal-to-noise ratio (SNR) fluorescence imaging using any magnification objective. As the incidence optics is perpendicular to the detector, label-free evanescent scattering-based imaging of submicron objects can also be performed without using emission filters. SNR is significantly enhanced in this case as compared to cTIRF alone, as seen through our model experiments performed on latex beads and mammalian cells. Extreme flexibility and the low cost of our approach makes it scalable for limited resource settings.

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“…Derived optical biosensors are integrated into microfluidic platforms adapted to measurements in a liquid medium, which results in the fabrication of miniaturized and portable sensors. Table 1 illustrates several examples of such biosensors cited in the literature [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ], and many other examples can be found in recent reviews [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Setting Up and Assessing a New Micro-Structured Waveguiding Fluorescent Architecture on Glass Entirely Elaborated by Sol–Gel Processing

et al. 2022

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Channel waveguides with diffraction gratings at their input and output for light injection and extraction, respectively, are extensively exploited for optical and photonic applications. In this paper, we report for the first time on such an architecture on glass entirely elaborated by sol–gel processing using a titanium-oxide-based photoresist that can be imprinted through a single photolithography step. This work is more particularly focused on a fluorescent architecture including channel waveguides doped with a ruthenium-complex fluorophore (tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II), Rudpp). The study demonstrates that this original sol–gel micro-structured architecture is well adapted to efficient channel waveguide/diffraction grating coupling and propagation of the fluorescence excitation and emission signals in the core of the channel waveguide. It demonstrates, in particular, a relatively large tolerance of several degrees in the angular injection fiber positioning and an important axial and vertical fiber spatial positioning tolerance of more than 100 µm at the Rudpp emission wavelength. The measurements also indicate that, in the conditions tested in this work, a Rudpp concentration of around 0.1 mM and a channel waveguide length of 2 to 5 mm offer the best trade-off in terms of excitation signal propagation and emission signal detection. This work constitutes a promising preliminary step toward the integration of our architecture into a microfluidic platform for fluorescence measurement in a liquid medium and waveguiding configuration.

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A portable optical waveguide resonance light-scattering scanner for microarray detection

Cited by 6 publications

References 50 publications

Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma

Polyacrylamide/Phytic Acid/Polydopamine Hydrogel as an Efficient Substrate for Electrochemical Enrichment of Circulating Cell-Free DNA from Blood Plasma

Compact 3D printed module for fluorescence and label-free imaging using evanescent excitation

Setting Up and Assessing a New Micro-Structured Waveguiding Fluorescent Architecture on Glass Entirely Elaborated by Sol–Gel Processing

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