A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection

Pancrazio, Joseph J.; Gray, Samuel A.; Shubin, Yura S.; Kulagina, N. V.; Cuttino, David S.; Shaffer, Kara M.; Eisemann, Kevin; Curran, Anthony; Zim, Bret; Gross, Guenter W.; O’Shaughnessy, Thomas J.

doi:10.1016/s0956-5663(03)00092-7

Cited by 112 publications

(71 citation statements)

References 18 publications

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“…Lower limits of detection for pesticides were achieved in the steady state system: carbaryl 26 nmol/L, heptenophos 14 nmol/L and fenitrothion 0.58 nmol/L. Similar multi-enzyme-based electrochemical biosensor arrays for the determination of pesticides [49][50][51][52] and phenols [53] have been reported by other workers.…”

Section: Enzyme Based Biosensor Arraysupporting

confidence: 68%

Biosensor Arrays for Environmental Monitoring

Song¹,

Si²,

Yu³

et al. 2011

Environmental Monitoring

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Section: Enzyme Based Biosensor Arraysupporting

confidence: 68%

Biosensor Arrays for Environmental Monitoring

Song¹,

Si²,

Yu³

et al. 2011

Environmental Monitoring

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“…Spatially organized field potential recordings of electrical activity from synchronized populations of neurons in brain slices using multi-electrode arrays (MEAs), have provided a powerful tool for research in this area (Kristensen et al, 2001;Thiebaud et al, 1999). Similarly, MEAs have been used to record activity from brain cells grown randomly in culture (Gopal, 2003;Pancrazio et al, 2003). However, the multiplexed signals acquired from such preparations are complex and difficult to interpret.…”

Section: Introductionmentioning

confidence: 99%

Cell placement and guidance on substrates for neurochip interfaces

Charrier

Martínez

Monette

et al. 2009

Biotech & Bioengineering

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Interface devices such as integrated planar patch‐clamp chips are being developed to study the electrophysiological activity of neuronal networks grown in vitro. The utility of such devices will be dependent upon the ability to align neurons with interface features on the chip by controlling neuronal placement and by guiding cell connectivity. In this paper, we present a strategy to accomplish this goal. Patterned chemical modification of SiN surfaces with poly‐d‐lysine transferred from PDMS stamps was used to promote adhesion and guidance of cryo‐preserved primary rat cortical neurons. We demonstrate that these neurons can be positioned and grown over microhole features which will ultimately serve as patch‐clamp interfaces on the chip. Biotechnol. Bioeng. 2010; 105: 368–373. © 2009 Wiley Periodicals, Inc.

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“…One example of such a cell-based biosensor that has been tested for field use incorporates cardiomyocytes that have been genetically engineered to more selectively respond to specific functional activity (15,65,105). An automated biosensor based on neuronal cells has also been tested and was found to respond to the biological toxins tetrodotoxin and tityustoxin (220,221). Neither of these biosensors has been explored for use with biological agents other than toxins.…”

Section: Tissue-and Cell-based Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection

Cited by 112 publications

References 18 publications

Biosensor Arrays for Environmental Monitoring

Biosensor Arrays for Environmental Monitoring

Cell placement and guidance on substrates for neurochip interfaces

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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