A portable fibre-probe ultraviolet light emitting diode (LED)-induced fluorescence detection system

Abstract

A portable fibre-probe fluorescence detection system comprising a continuous-wave high-power ultraviolet light emitting diode (UV LED) emitting at 365 nm as excitation source, a bifurcated fibre probe with a six-around-one fibre configuration to illuminate and read from a large target area (∼3.6 mm2) and an integrated PC-coupled spectrometer has been developed. The construction, calibration and operation of the fluorescence detection system are described. Demonstrative test measurements with the system for possible inspection of different ripening stages on some batches of horticultural and agricultural products (lemon, mandarin, banana leaf and ivy leaf) have been performed and results presented. The system is portable, comparatively low cost, easily operated and relative immune to ambient light, thus being suitable for field measurements.

Autofluorescence Detection in Analytical Chemistry and Biochemistry

“…Recently, high-power LEDs emitting in the near and deep UV have become commercially available (154). Employing UV LEDs as an excitation source in a portable fluoresensor has been reported (155,156). The second modification is to combine optical components with microchip plate.…”

Realistic Instrumentation Platform for Active and Passive Optical Remote Sensing

“…The first cases use ambient light for monitoring flying insects, 17 followed by descriptions of different experiments ranging from insect wing-beat studies to fluorescence characterization of vegetation, 22 and assessment of water quality by Raman spectroscopy. Background material to this work is given in insects, 16–20 vegetation, 12,13,21 and water. 22–25…”

Investigation of the crystal structure and electrochemical properties of catena-poly[[chlorido(piperazin-1-ium–κN))cadmium(II)]-di-μ-chlorido]monohydrate

“…Thermal analysis was performed with a multimodule 92 Setaram analyzer, under a nitrogen atmosphere, in the temperature range 25-450°C, at a heating rate of 10°min -1 . Measurement of fluorescence was performed with a portable fibre-probe fluorescence detection system comprising a continuous wave high-power ultraviolet (UV) light-emitting diode (LED) with a peak wavelength of 365 nm and 8 nm spectral bandwidth [18]. A voltammetric study was undertaken with a Tacussel potentiostat (PGP 201).…”