A portable electrochemical immunosensor for rapid detection of trace aflatoxin B<sub>1</sub> in rice

Li, Zhanming; Ye, Zunzhong; Fu, Yingchun; Xiong, Yonghua; Li, Yanbin

doi:10.1039/c5ay02643a

Cited by 39 publications

(18 citation statements)

References 38 publications

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“…In comparison with data reported in previous literature, displayed in Table 1, the performances of the CD-trode sensor are comparable with those obtained with some of the immunosensors previously described in the literature [20,22,25], but with the advantage of being based on the use of disposable and low-cost CD-trodes. These preliminary results are encouraging in order to progress with an in-depth validation of the sensor, in particular concerning matrix effects and recovery tests.…”

Section: Discussionsupporting

confidence: 74%

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

et al. 2016

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This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B 1 (AFB 1 ). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B 1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB 1 . The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB 1 antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 × 10 −9 to 1 × 10 −7 mol·L −1 (1.56-31.2 ng·mL −1 ), limit of detection and limit of quantification, respectively, 3.6 × 10 −10 and 1.1 × 10 −9 mol·L −1 (0.11 and 0.34 ng·mL −1 ). The proposed immunosensor was applied to analyze peanut samples.

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Section: Discussionsupporting

confidence: 74%

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

et al. 2016

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“…Their products encompass disposable SPEs with different electrode materials (e.g., graphite, gold, platinum) or even nano-engineered surfaces (e.g., deposited gold nanoparticles or carbon nanotubes). Working electrodes with a diameter of only a few millimeters enable miniaturized assays [30].…”

Section: Introductionmentioning

confidence: 99%

“…In this way, a range of even complex 3D structures can be manufactured. The 3D-printed open cells may include a chamber for screen-printed microinterdigitated electrodes, which also enable a connection via Universal Serial Bus (USB) [30] and a chamber for bipolar electrodes [46]. An example of a 3D-printed microfluidic chamber was presented by Damiati et al The SPE and the microfluidic chamber were bonded by a double-sided adhesive layer [37].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Siller

Preuß

Urmann

et al. 2020

Sensors

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Electrochemical spectroscopy enables rapid, sensitive, and label-free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen-printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio-)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high-resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor-made, experiment-specific systems. In this work, flow cells for SPE-based electrochemical (bio-)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamer-based impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D-printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high-resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio-)sensor applications.

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“…In this sense, only few attempts of potentiostat miniaturization can be found in the literature [6][7][8][9], and some important concerns always arise. Some authors focused on adapting already existing portable electronics to perform cyclic voltammetry.…”

Section: Introductionmentioning

confidence: 99%

“…As for portable impedance measurements, an interesting work is presented by Zhang et al [7], who, according to the same philosophy, integrated a commercial miniaturized impedance analyzer AD5933 (Analog Devices, Norwood, MA, USA) with an Arduino UNO board and a Bluetooth module for BSA immunosensing applications. Similarly, Li et al [8] developed a detector combined with a 3D-printed USB-compatible chip to sense aflatoxins in rice. They benchmarked their device against a Zahner commercial station, achieving good limit of detection.…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Impedance Measurements for Lab-on-a-Chip Architectures: Towards Potentiostat Miniaturization

Pruna

Palacio

Baraket

et al. 2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Abstract:The development of miniaturized potentiostats capable of measuring in a wide range of conditions and with full characteristics (e.g., wide bandwidth and capacitive/inductive contribution to sensor's impedance) is still an unresolved challenge in bioelectronics. We present a simple analogue design coupled to a digital filter based on a lock-in amplifier as an alternative to complex architectures reported hitherto. A low-cost, miniaturized and fully integrated acquisition electronic system was developed, tested for a fully integrated three-lead electrochemical biosensor and benchmarked against a commercial potentiostat. The portable potentiostat was coupled to an array of miniaturized gold working electrodes to perform complex impedance analyses for tumor necrosis factor α (TNF-α) cytokine detection. This wearable potentiostat is very promising for the development of low-cost point-of-care (POC) with low power consumption.

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A portable electrochemical immunosensor for rapid detection of trace aflatoxin B₁ in rice

Abstract: To explore the possibility of achieving rapid and in situ detection of aflatoxin B1 (AFB1), a portable biosensing instrument consisting of an impedance detector and a 3D-printed USB-compatible sensor chip was developed.

Cited by 39 publications

References 38 publications

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Low-Cost Impedance Measurements for Lab-on-a-Chip Architectures: Towards Potentiostat Miniaturization

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A portable electrochemical immunosensor for rapid detection of trace aflatoxin B1 in rice

Abstract: To explore the possibility of achieving rapid and in situ detection of aflatoxin B1 (AFB1), a portable biosensing instrument consisting of an impedance detector and a 3D-printed USB-compatible sensor chip was developed.

Cited by 39 publications

References 38 publications

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Low-Cost Impedance Measurements for Lab-on-a-Chip Architectures: Towards Potentiostat Miniaturization

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A portable electrochemical immunosensor for rapid detection of trace aflatoxin B₁ in rice