A portable approach for the surveillance of dengue virus-infected mosquitoes

Muller, David A.; Frentiu, Francesca D.; Rojas, Alejandra; Moreira, Luciano Andrade; O’Neill, Scott L.; Young, Paul R.

doi:10.1016/j.jviromet.2012.03.033

Cited by 21 publications

(20 citation statements)

References 28 publications

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“…Clearly, the Platelia Dengue NS1 Ag kit or other NS1 assays could be fruitfully applied to DENV surveillance in Ae. aegypti and could become a significant addition to the armamentarium of vector surveillance and control programs as previously suggested by Tan and others 28 and Muller and others 29 The Platelia Dengue NS1 Ag kit proved to be a simpler, more rapid, and less laborious test for detection of DENV-infected mosquitoes than either virus isolation or RT-PCR/nested PCR. Theoretically, the Platelia Dengue NS1 Ag kit could be more cost-effective than the other tests because up to 92,000 mosquitoes could be tested with a single plate using pools of 1,000.…”

Section: Discussionmentioning

confidence: 79%

“…This portable test could be performed in 30 min, allowing for rapid monitoring of DENV in mosquitoes in the field. 29 We expanded upon these studies and evaluated the use of a third test-the Platelia Dengue NS1 Ag kit (Bio-Rad Laboratories; Catalogue no. 72830)-to detect DENV NS1 antigen in mosquitoes subjected to conditions and processing steps commonly encountered in mosquito-based surveillance systems.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Voge¹,

Sánchez-Vargas²,

Blair³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/ pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Voge¹,

Sánchez-Vargas²,

Blair³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…aegypti females is of potential interest as a means for early detection of dengue outbreaks because human DENV infections are commonly asymptomatic or “silent”(Gubler 1998, Kyle and Harris 2008), which decreases the effectiveness of dengue case surveillance to provide early warning of building outbreaks. Improved methods for detection of DENV RNA or antigen in mosquitoes (Bangs et al 2007, Chao et al 2007, Chen et al 2010, Tan et al 2011, Muller et al 2012, Voge et al 2013) provide new opportunities for mosquito-based DENV surveillance, and studies focusing entirely or in part on dengue patient premises have shown promise with regards to the potential for linking DENV infections in Ae. aegypti to human dengue cases in space and time (Pinheiro et al 2005, Urdaneta et al 2005, García-Rejón et al 2008, Guedes et al 2010, Yoon et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Temporal Correlations Between Mosquito-Based Dengue Virus Surveillance Measures or Indoor Mosquito Abundance and Dengue Case Numbers in Mérida City, México

Eisen¹,

García-Rejón²,

Gómez-Carro³

et al. 2014

J Med Entomol

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Surveillance of dengue virus (DENV) in Aedes (Stegomyia) aegypti (L.) females is of potential interest because human DENV infections are commonly asymptomatic, which decreases the effectiveness of dengue case surveillance to provide early warning of building outbreaks. Our primary aim was to examine if mosquito-based virological measures – monthly percentages of examined Ae. aegypti females infected with DENV or examined homes from which ≥1 DENV-infected Ae. aegypti female was collected – are correlated with reported dengue cases in the same or subsequent months within study neighborhoods in Mérida City, México. The study encompassed ~30 neighborhoods in the southern and eastern parts of the city. Mosquitoes were collected monthly over a 15-month period within study homes (average of 145 homes examined per month); this produced ~5,800 Ae. aegypti females subsequently examined for DENV RNA. Although monthly dengue case numbers in the study neighborhoods varied more than 100-fold during the study period, we did not find statistically significant positive correlations between monthly data for mosquito-based DENV surveillance measures and reported dengue cases in the same or subsequent months. Monthly average temperature, rainfall, and indoor abundance of Ae. aegypti females were positively correlated (P ≤ 0.001) with dengue case numbers in subsequent months with lag times of 3–5, 2, and 1–2 months, respectively. However, because dengue outbreak risk is strongly influenced by serotype-specific susceptibility of the human population to DENV, the value of weather conditions and entomological indices to predict outbreaks is very limited. Potential ways to improve the sensitivity of mosquito-based DENV surveillance are discussed.

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“…It plays important role in pathogenesis and cellular profusion and acts as a virulence determinant [117–119], and serves as a marker of Dengue virus infection in man [120] and mosquitoes [121]. Both secreted and cell-surface-associated NS1 are highly immunogenic and implicate disease pathogenesis [122–126].…”

Section: Virus Proteins As Biomarkersmentioning

confidence: 99%

Biomarkers in Japanese Encephalitis: A Review

Upadhyay

2013

BioMed Research International

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JE is a flavivirus generated dreadful CNS disease which causes high mortality in various pediatric groups. JE disease is currently diagnosed by measuring the level of viral antigens and virus neutralization IgM antibodies in blood serum and CSF by ELISA. However, it is not possible to measure various disease-identifying molecules, structural and molecular changes occurred in tissues, and cells by using such routine methods. However, few important biomarkers such as cerebrospinal fluid, plasma, neuro-imaging, brain mapping, immunotyping, expression of nonstructural viral proteins, systematic mRNA profiling, DNA and protein microarrays, active caspase-3 activity, reactive oxygen species and reactive nitrogen species, levels of stress-associated signaling molecules, and proinflammatory cytokines could be used to confirm the disease at an earlier stage. These biomarkers may also help to diagnose mutant based environment specific alterations in JEV genotypes causing high pathogenesis and have immense future applications in diagnostics. There is an utmost need for the development of new more authentic, appropriate, and reliable physiological, immunological, biochemical, biophysical, molecular, and therapeutic biomarkers to confirm the disease well in time to start the clinical aid to the patients. Hence, the present review aims to discuss new emerging biomarkers that could facilitate more authentic and fast diagnosis of JE disease and its related disorders in the future.

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A portable approach for the surveillance of dengue virus-infected mosquitoes

Cited by 21 publications

References 28 publications

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Temporal Correlations Between Mosquito-Based Dengue Virus Surveillance Measures or Indoor Mosquito Abundance and Dengue Case Numbers in Mérida City, México

Biomarkers in Japanese Encephalitis: A Review

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