A porphycene-gentamicin conjugate for enhanced photodynamic inactivation of bacteria

Nieves, Ingrid; Hally, Cormac; Viappiani, Cristiano; Agut, Montserrat; Nonell, Santi

doi:10.1016/j.bioorg.2020.103661

Cited by 26 publications

(26 citation statements)

References 47 publications

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“…Dastgheyb et al, Ronqui et al, and Nieves et al investigated not only strains of E. coli but also S. aureus. As has been previously commented, results are similar in both species [ 27 , 32 , 35 ].…”

Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuessupporting

confidence: 85%

“…It outperforms the antimicrobial effect against S. aureus and E. coli of the two components delivered separately. This novel photoantimicrobial agent may be used to enhance the therapeutic index of gentamicin, broaden the spectrum of pathogens against which it is effective, and reduce its side effects [ 27 ].…”

Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuesmentioning

confidence: 99%

“…The gentamicin concentration used was close to the reported MIC (3.8 μg/mL and 4 μg/mL respectively). Nevertheless, MIC values were determined at inoculum sizes 100–1000-fold, more diluted than those used in this study, and the antimicrobial effect usually was evaluated after 24 h of incubation with the antibiotic, while only 1 h of preirradiation incubation was allowed in these experiments; therefore, it is not surprising the lack of gentamicin toxicity in darkness [ 27 ].…”

Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuesmentioning

confidence: 99%

See 2 more Smart Citations

Photodynamic Therapy Combined with Antibiotics or Antifungals against Microorganisms That Cause Skin and Soft Tissue Infections: A Planktonic and Biofilm Approach to Overcome Resistances

et al. 2021

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The present review covers combination approaches of antimicrobial photodynamic therapy (aPDT) plus antibiotics or antifungals to attack bacteria and fungi in vitro (both planktonic and biofilm forms) focused on those microorganisms that cause infections in skin and soft tissues. The combination can prevent failure in the fight against these microorganisms: antimicrobial drugs can increase the susceptibility of microorganisms to aPDT and prevent the possibility of regrowth of those that were not inactivated during the irradiation; meanwhile, aPDT is effective regardless of the resistance pattern of the strain and their use does not contribute to the selection of antimicrobial resistance. Additive or synergistic antimicrobial effects in vitro are evaluated and the best combinations are presented. The use of combined treatment of aPDT with antimicrobials could help overcome the difficulty of fighting high level of resistance microorganisms and, as it is a multi-target approach, it could make the selection of resistant microorganisms more difficult.

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Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuessupporting

confidence: 85%

Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuesmentioning

confidence: 99%

Section: Results Of Studies On In Vitro Apdt Combined With Antimicrobial Agents Against Infectious Microorganism Of Skin and Soft Tissuesmentioning

confidence: 99%

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Photodynamic Therapy Combined with Antibiotics or Antifungals against Microorganisms That Cause Skin and Soft Tissue Infections: A Planktonic and Biofilm Approach to Overcome Resistances

et al. 2021

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“…The mode of action is based on impedimentation of the initiation and further translation of protein synthesis by binding to 30S and 16S ribosomal RNA [ 79 , 80 ]. The co-administration of this antibiotic with Rose Bengal (RB) in aPDT has been studied by Perez-Laguna et al [ 81 ], and following their work, Nonell and co-workers demonstrated the use of gentamicin as a targeting unit in a covalent conjugation strategy [ 82 ] ( Figure 5 ). Their gentamicin aPDT probe, used the red-light-absorbing PS porphycene and showed significant eradication of both Gram+ ( S. aureus ) and Gram− ( E. coli ) strains even at sub-micromolar concentrations while the controls (PS alone and co-administered with gentamicin) did not impact significantly on survival rate.…”

Section: Recent Studies On Targeted Apdtmentioning

confidence: 99%

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

2020

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Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2−, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.

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“…Gentamicin is an aminoglycoside used for the treatment of a broad range of systemic bacterial infections (conjugate 4, Figure 4A). 34 Further studies illustrated the selective photoinactivation of E. coli and S. aureus by 4 relative to controls (Figures 4B and 4C). This promising…”

Section: Porphycenesmentioning

confidence: 87%

Porphyrinoid Drug Conjugates

Arambula

Sessler

2020

Chem

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Drawing inspiration from nature today remains a time-honored means of discovering the therapies of tomorrow. Porphyrins, the so-called ''pigments of life'' have played a key role in this effort due to their diverse and unique properties. They have seen use in a number of medically relevant applications, including the development of so-called drug conjugates wherein functionalization with other entities is used to improve efficacy while minimizing dose limiting side effects. In this Perspective, we highlight opportunities associated with newer, completely synthetic analogs of porphyrins, commonly referred to as porphyrinoids, as the basis for preparing drug conjugates. Many of the resulting systems show improved medicinal or site-localizing properties. As befits a Perspective of this type, our efforts to develop cancer-targeting, platinum-containing conjugates based on texaphyrins (a class of so-called ''expanded porphyrins'') will receive particular emphasis; however, the promise inherent in this readily generalizable approach will also be illustrated briefly using two other common porphyrin analogs, namely the corroles (a ''contracted porphyrin'') and porphycene (an ''isomeric porphyrin'').

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A porphycene-gentamicin conjugate for enhanced photodynamic inactivation of bacteria

Cited by 26 publications

References 47 publications

Photodynamic Therapy Combined with Antibiotics or Antifungals against Microorganisms That Cause Skin and Soft Tissue Infections: A Planktonic and Biofilm Approach to Overcome Resistances

Photodynamic Therapy Combined with Antibiotics or Antifungals against Microorganisms That Cause Skin and Soft Tissue Infections: A Planktonic and Biofilm Approach to Overcome Resistances

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Porphyrinoid Drug Conjugates

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