A porcine-cholecyst-derived scaffold for treating full thickness lacerated skin wounds in dogs

Abstract

In regenerative medicine, despite the chances of graft-rejection, scaffolds prepared from extracellular matrices of various mammalian organs/tissues are widely used. Graft-assisted healing of full thickness skin-wounds is a major use of these bioscaffolds. Therefore, considering its prospective clinical use as a wound healing matrix, this study evaluated the healing potential of porcine cholecyst-derived scaffold (CDS) prepared by a non-detergent/enzymatic method for treating naturally occurring full thickness lacerated wounds in dogs. The CDS caused, in comparison with a commercial-grade bioscaffold prepared out of bovine dermal collagen (BDC), faster healing with respect to the wound healing parameters like peripheral tissue oedema, necrosis (amount and type), indurations, granulation tissue formation and the extent of re-epithelialisation. After 28 days of the treatment, the wound area (mean + SE) reduced from 27.60 ± 8.96 cm to 0.19+ 0.18 cm and 21.39 ± 5.48 to 6.59 ± 2.60 cm in CDS and BDC treated animals, with a reduction in wound sizes by 98.95 ± 2.09% and 54.53 ± 15.90 respectively. By this time, complete wound healing was observed in at least 75% of the former and 25% of the later groups. The CDS was deemed as a candidate bioscaffold for treating full thickness lacerated skin wounds in dogs.

Gelatin-Modified Cholecyst-Derived Scaffold Promotes Angiogenesis and Faster Healing of Diabetic Wounds

“…The conventional approach for scaffold modifications, directed toward augmentation of angiogenesis, involves supplementation with micromolecules that support angiogenesis. , However, most animal-derived extracellular matrices, including CDS, are excellent scaffolds for wound healing applications because of the rich content of natural biomolecules. ,, To compensate the perceived loss of pro-angiogenic factors happened during the CDS preparation procedure, we preferred to modify it with gelatin, which has exposed RGD sequences that facilitate interaction with αvβ3 integrin of endothelial cells. , We believe that a combination of the extracellular matrix components of the CDS and gelatin can enhance angiogenesis in diabetic wounds.…”

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

“…For the simplest tissues, this can often be achieved by a chemical bath. Tissues such as urinary bladders and gallbladders, small intestines, and joint capsules can be processed by removing all the tissue layers and leaving the lamina propria, submucosa, and synovium, respectively, and washing them with a weak solution of peracetic acid and ethanol [ 23 , 82 , 92 , 128 , 158 , 195 , 196 , 197 , 200 , 223 , 224 , 225 , 227 , 230 ].…”

Regenerative Methoden in der Tiermedizin

“…Durch ihre strukturellen und molekularen Eigenschaften können sie über die mechanische Stabilisierung hinaus die Heilung positiv beeinflussen [3]. Der Einsatz von extrazellulärer Matrix ist in der regenerativen Chirurgie in der Humanmedizin nicht mehr wegzudenken [4][5] in der Veterinärmedizin spielt er -wohl auch aus Kostengründen -noch eine untergeordnete Rolle, erfolgt aber beispielsweise unter Verwendung von Submukosa für die rekonstruktion chronischer Wunden [6] und in der Ophthalmologie [7] sowie unter Verwendung von demineralisierter Knochenmatrix in der orthopädischen Chirurgie [8].…”