A population genomics approach to unlock the genetic potential of lablab (Lablab purpureus), an underutilized tropical forage crop

Abstract

BackgroundIn Sub-Saharan Africa (SSA) livestock production and productivity are severely restricted by the scarce supply of feedstuffs and forage crops, while those available are often of low nutritional quality resulting in poor animal productivity and leading to widespread malnutrition among the public, particularly women and children. Traditionally, several tropical forage crops have been used in the region both in the rangelands and as cut-and-carry cropping systems, but limited research attention has been paid to improve the quality of and access to these animal feeds. Lablab (Lablab purpureus(L.) is one of the conventionally grown multi-purpose underutilized crops that originated in Africa. It is an annual or short-lived perennial multi-purpose forage legume which has versatile uses (as a vegetable and dry seed), and as feed for animals, or as green manure. To develop new and highly productive lablab varieties, using genomics-assisted selection, the present study aimed to identify quantitative trait loci (QTL) associated with agronomically important traits in lablab and to assess the stability of these traits across two different agro-ecologies in Ethiopia.ResultsOne hundred and forty-two cultivated and wild lablab accessions displayed significant agro-morphological variation in eight analysed traits, including plant height, total fresh weight, and total dry weight. Further, the agronomic performance of the accessions was significantly different across locations and years, highlighting the substantial genotype-by-environment interactions. The population genetic structure of the lablab accessions, based on half a million high quality single nucleotide polymorphisms (SNPs), revealed an independent domestication pattern for two-seeded and four-seeded lablab accessions. Furthermore, based on multi-environmental trial data, a genome-wide association study (GWAS) identified useful SNPs andk-mers for yield-related traits, such as plant height and total dry weight.ConclusionsGenomic-assisted breeding is playing a key role in accelerating trait improvement in temperate forages, such as perennial ryegrass and alfalfa. Here we show that a similar approach could benefit underutilized crops such as lablab. The publicly available genomic tools and field evaluation data from this study will offer a valuable resource for plant breeders and researchers, to initiate genomic-assisted breeding in lablab which will fast-track genetic gain per unit time and ultimately contribute towards achieving food/nutritional security in the region.