A pomegranate seed-structured nanozyme-based colorimetric immunoassay for highly sensitive and specific biosensing of <i>Staphylococcus aureus</i>

Li, Jinghui; Tang, Yipeng; Bai, Yunpeng; Zhang, Zhejun; Zhang, Shaopeng; Chen, Tongyun; Zhao, Feng; Guo, Zhigang

doi:10.1039/d3an01621h

Cited by 2 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Until now, most of the reported bacterial sensors were based on the biorecognition elements, such as aptamers, antibodies, vancomycin, bacteriophages, etc. Among them, bacteriophages are a class of viruses that specifically target bacteria without posing any harm to the human body, offer distinct advantages due to their abundance in nature, robust proliferative capabilities, cost-effective, and greater tolerance to pH and temperature variations, resulting in superior stability .…”

Section: Introductionmentioning

confidence: 99%

Photoelectrochemical/Colorimetric Dual-Mode Specific Detection of Staphylococcus aureus Based on the Enzymatic Reaction Triggered by Catalase from Lysed Bacteria

Zeng,

Zou,

Deng

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Staphylococcus aureus (S. aureus) is abundant in nature and frequently leads to various health issues. Bacteriophages as obligate intracellular parasites of bacteria have the ability to specifically identify and infect S. aureus, causing bacterial lysis and the release of endogenous catalase (CAT). The released CAT triggers the conversion of H 2 O 2 into O 2 and H 2 O, resulting in a notable decrease in UV absorption at 570 nm and a concurrent surge in photocurrent. On the basis of this, a photoelectrochemical/ colorimetric dual-mode biosensor for the detection of S. aureus was developed. In the photoelectric detection mode, the reactions involving endogenous enzymes occur directly in the solution, requiring only the simple drop-coating of TiO 2 @CdS onto the indium tin oxide (ITO) electrode surface. There was no need for immobilizing additional biomolecules, thereby significantly minimizing nonspecific adsorption and improving the biosensor's stability and reproducibility. For colorimetry, we utilized a cost-effective and operationally simple approach based on KI and starch. Remarkably, this photoelectrochemical/colorimetry exhibited a linear range of 10 2 −10 9 CFU/mL for S. aureus, achieving detection limits of 7 and 10 CFU/mL, respectively. Herein, phage identification ensures the specific detection of live S. aureus, thereby effectively mitigating the potential for false signals. The dual-signal readout mode improves the detection accuracy and reliability. In conclusion, this present method offers numerous advantages, including simplicity, time-efficiency, cost-effectiveness, high specificity, and therefore excellent accuracy.

show abstract