A Polyplatin with Hands‐Holding Near‐Infrared‐II Fluorophores and Prodrugs at a Precise Ratio for Tracking Drug Fate with Realtime Readout and Treatment Feedback

Abstract

The in vivo fate of chemotherapeutic drugs plays a vital role in helping the understanding of the therapeutic outcome, side effects, and the mechanism of action. However, the lack of imaging abilities of most drugs, tedious labelling processes, and premature leakage of imaging agents currently result in loss of fidelity between the drugs and any imaging signals. Herein, an unique amphiphilic polymer was created by copolymerization of a NIR‐II fluorophore drug tracer (T) and an anticancer Pt(IV) prodrug (D) of cisplatin in a hand‐holding manner into one polymer chain for the first time. The obtained PolyplatinDT was capable of delivering the drugs and the fluorophores concomitantly at a precise D/T ratio, thereby resulting in tracking the platinum drugs and even readout of them in real‐time via NIR‐II fluorescence imaging both in vitro and in vivo. PolyplatinDT could self‐assemble into nanoparticles, referred to as NanoplatinDT. Furthermore, a caspase‐3 cleavable peptide with quenched FRET pairs that serves as an apoptosis protein reporter was attached to NanoplatinDT, resulting in NanoplatinDTR that were capable of simultaneously tracking platinum drugs with NIR‐II fluorescence and evaluating the therapeutic efficacy with the apoptosis reporters. The strong correlations between the fluorescence intensity of the NIR‐II fluorophores and 5′‐FAM, and the Pt levels were investigated both in vitro and in vivo with NanoplatinDTR, enabling a quantitative readout of the platinum levels and feasible evaluation of treatment efficacy. Overall, we reported here the design of the first theranostic polymer with anticancer drugs, drug tracers, and drug efficacy reporters that could work in concert to provide possible insight into the drug fate and mechanism of action in vitro and in vivo.This article is protected by copyright. All rights reserved

Silver incorporated SeTe nanoparticles with enhanced photothermal and photodynamic properties for synergistic effects on anti-bacterial activity and wound healing

“…When the biolms were formed on the walls, the media was removed by gently inverting the plate, and PBS was used to wash the biolm and remove the planktonic cells. Aerward, different concentrations of SeTe NPs and SeTe-Ag NPs prepared in PBS (20,40,80, and 100 mg mL −1 ) were added to the wells and incubated, while taking PBS treated biolm as negative control. Then, methanol was added to x the biolm for 15 min and treated for 15 min with crystal violet (0.5%) prepared in PBS.…”

“…Live/dead assay. Finally, live/dead staining DAPI and PI probes 39,40 were used to assess the bactericidal effect of SeTe-Ag NPs. As shown in Fig.…”