A Polyphenol-Based Multicomponent Nutraceutical in Dysmetabolism and Oxidative Stress: Results from a Pilot Study

Corsi, Roberto; Mosti, Giovanni; Cavezzi, Attilio; Urso, S; Dimitrova, Gayla; Fioroni, E; Colucci, Roberto; Quinzi, Valentina

doi:10.1080/19390211.2017.1310784

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…These intervention studies mainly showed beneficial effects of enriched olive oil on several key processes related to cardiovascular risk [49,50]. In other studies, it was shown the ability of a multicomponent nutraceutical containing HT to reduce free radicals and oxidized cholesterol, and to increase the serum antioxidant capacity [51]. An olive leaf extract (OLE) containing HT was used in a randomised, controlled, double-blind, crossover intervention trial to test its anti-inflammatory abilities in pre-hypertensive male volunteers.…”

Section: Discussionmentioning

confidence: 99%

Hydroxytyrosol Exerts Anti-Inflammatory and Anti-Oxidant Activities in a Mouse Model of Systemic Inflammation

2018

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Hydroxytyrosol (3,4-dihydroxyphenil-ethanol, HT), the major phenol derived from olive oil consumption, has shown different anti-inflammatory and anti-oxidant activities in vitro which may explain the chronic-degenerative diseases preventive properties of olive oil. The aim of this study was to examine the ability of HT reduce inflammatory markers, Cyclooxygenase-2 (COX2) and Tumour Necrosis Factor alfa (TNF-α and oxidative stress in vivo on a mouse model of systemic inflammation. Balb/c mice were pre-treated with HT (40 and 80 mg/Kg b.w.) and then stimulated by intraperitoneal injection of lipopolysaccharide (LPS). Blood was collected to measure COX2 gene expression by qPCR and TNF-α level by ELISA kit in plasma. In addition, the total anti-oxidant power of plasma and the DNA damage were measured by FRAP test and COMET assay, respectively. LPS increased the COX2 expression, the TNF-α production and the DNA damage. HT administration prevented all LPS-induced effects and improved the anti-oxidant power of plasma. HT demonstrated in vivo anti-inflammatory and anti-oxidant abilities. The results may explain the health effects of olive oil in Mediterranean diet. HT represents an interesting molecule for the development of new nutraceuticals and functional food useful in chronic diseases prevention.

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Section: Discussionmentioning

confidence: 99%

Hydroxytyrosol Exerts Anti-Inflammatory and Anti-Oxidant Activities in a Mouse Model of Systemic Inflammation

2018

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“…MqB treatment counteracted hyperlipidemia, hyperglycemia, and ROS production in metabolic syndrome patients. An MqB polyphenol-based-nutraceutical reversed low-grade-inflammation, oxidative stress, and atherosclerogenesis in pre-diabetic patients [ 223 ]. MqBE and purified anthocyanin consumption showed positive outcomes for post-stroke stress and depression in diabetic mice.…”

Section: Maqui Berriesmentioning

confidence: 99%

Select Polyphenol-Rich Berry Consumption to Defer or Deter Diabetes and Diabetes-Related Complications

et al. 2020

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Berries are considered “promising functional fruits” due to their distinct and ubiquitous therapeutic contents of anthocyanins, proanthocyanidins, phenolic acids, flavonoids, flavanols, alkaloids, polysaccharides, hydroxycinnamic, ellagic acid derivatives, and organic acids. These polyphenols are part of berries and the human diet, and evidence suggests that their intake is associated with a reduced risk or the reversal of metabolic pathophysiologies related to diabetes, obesity, oxidative stress, inflammation, and hypertension. This work reviewed and summarized both clinical and non-clinical findings that the consumption of berries, berry extracts, purified compounds, juices, jams, jellies, and other berry byproducts aided in the prevention and or otherwise management of type 2 diabetes mellitus (T2DM) and related complications. The integration of berries and berries-derived byproducts into high-carbohydrate (HCD) and high-fat (HFD) diets, also reversed/reduced the HCD/HFD-induced alterations in glucose metabolism-related pathways, and markers of oxidative stress, inflammation, and lipid oxidation in healthy/obese/diabetic subjects. The berry polyphenols also modulate the intestinal microflora ecology by opposing the diabetic and obesity rendered symbolic reduction of Bacteroidetes/Firmicutes ratio, intestinal mucosal barrier dysfunction-restoring bacteria, short-chain fatty acids, and organic acid producing microflora. All studies proposed a number of potential mechanisms of action of respective berry bioactive compounds, although further mechanistic and molecular studies are warranted. The metabolic profiling of each berry is also included to provide up-to-date information regarding the potential anti-oxidative/antidiabetic constituents of each berry.

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“…These extracts, which boost the activity of vitamin E present in feeds, may also exert the observed antioxidant activity by means of different mechanisms of action, including radical scavenging and enhancement of the expression of antioxidant enzymes [ 64 ]. Polyphenols have been extensively studied and reported to improve the oxidative status of cells in culture [ 65 , 66 ] but also animals (for references see [ 67 ]) and humans [ 68 , 69 ]. Our setup allowed the identification of an effective antioxidant concentration of ELIFE in a simple and fast way (total ROS stimulation and assessment time is about 4.5 h, of which 1 h is hands-on).…”

Section: Discussionmentioning

confidence: 99%

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

Ayuso

Cruchten

Ginneken

2020

IJMS

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The feed industry continuously seeks new molecules with antioxidant capacity since oxidative stress plays a key role in intestinal health. To improve screening of new antioxidants, this study aims to set up an assay to assess oxidative stress in the porcine small intestinal epithelial cell line IPEC-J2 using plate-reader-based analysis of fluorescence. Two oxidants, H2O2 and menadione, were tested at 1, 2 and 3 mM and 100, 200 and 300 µM, respectively. Trolox (2 mM) was used as the reference antioxidant and the probe CM-H2DCFDA was used to indicate intracellular oxidative stress. Cell culture, reactive oxygen species (ROS) production and assessment conditions were optimized to detect a significant ROS accumulation that could be counteracted by pre-incubation with trolox. Menadione (200 µM) reproducibly increased ROS levels, H2O2 failed to do so. Trolox significantly decreased intracellular ROS levels in menadione (200 µM)-exposed cells in a consistent way. The system was further used to screen different concentrations of the commercially available antioxidant ELIFE®. Concentrations between 100 and 200 ppm protected best against intracellular ROS accumulation. In conclusion, the combination of CM-H2DCFDA fluorescence analysis by a plate-reader, trolox as a reference antioxidant and 200 µM of menadione as a stressor agent, provides a replicable and reliable medium-throughput setup for the evaluation of intracellular oxidative stress in IPEC-J2 cells.

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A Polyphenol-Based Multicomponent Nutraceutical in Dysmetabolism and Oxidative Stress: Results from a Pilot Study

Cited by 6 publications

References 17 publications

Hydroxytyrosol Exerts Anti-Inflammatory and Anti-Oxidant Activities in a Mouse Model of Systemic Inflammation

Hydroxytyrosol Exerts Anti-Inflammatory and Anti-Oxidant Activities in a Mouse Model of Systemic Inflammation

Select Polyphenol-Rich Berry Consumption to Defer or Deter Diabetes and Diabetes-Related Complications

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

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