1996
DOI: 10.1074/jbc.271.18.10892
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A Polymorphism in the Human Intestinal Fatty Acid Binding Protein Alters Fatty Acid Transport across Caco-2 Cells

Abstract: The human intestinal fatty acid binding protein (IFABP) binds long-chain fatty acids in vitro, but its intracellular function has remained speculative. A polymorphism in the gene that encodes IFABP results in an alanine (Ala54) to threonine (Thr54) substitution at codon 54 that alters the in vitro binding affinity of the protein for long-chain fatty acids. To identify potential functional variability between Ala54 and Thr54 IFABP, we established permanently transfected Caco-2 cell lines that express either Ala… Show more

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Cited by 160 publications
(130 citation statements)
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“…In an abstract, Rolf et al [54] reported a reduction of fatty acid uptake into HepG2 liver cells transfected with antisense L-FABP cDNA and a decreased L-FABP content. Furthermore the total amount of L-FABP and I-FABP in I-FABP-cDNA-transfected Caco-2 cells was not proportional to fatty acid uptake [55]. CHO cell transfectants expressing A-FABP demonstrated a 1n5-2-fold increase in the rate of [$H]oleate uptake [26].…”
Section: Discussionmentioning
confidence: 99%
“…In an abstract, Rolf et al [54] reported a reduction of fatty acid uptake into HepG2 liver cells transfected with antisense L-FABP cDNA and a decreased L-FABP content. Furthermore the total amount of L-FABP and I-FABP in I-FABP-cDNA-transfected Caco-2 cells was not proportional to fatty acid uptake [55]. CHO cell transfectants expressing A-FABP demonstrated a 1n5-2-fold increase in the rate of [$H]oleate uptake [26].…”
Section: Discussionmentioning
confidence: 99%
“…The T54 variant of FABP2 shows increased binding affinity and intestinal uptake of long chain fatty acids, 30,31 and has previously been found to be associated with increased plasma triglyceride concentrations, 32 increased BMI and increased percent body fat. 23 Thus, an association might be expected between HTGW and FABP2.…”
Section: Discussionmentioning
confidence: 99%
“…The G to A polymorphism of codon 54 of the FABP 2 gene, resulting in the substitution of threonine (Thr) for alanine (Ala) in I-FABP, has been reported as showing increased affinity of I-FABP for long-chain FA 3) and increased triglyceride secretion by Caco-2 cells 4) . Among Pima Indians, who are known to have the highest prevalence of type 2 diabetes mellitus, the Thr encoding allele (Thr54) is associated with insulin resistance and enhanced fat oxidation rates 4,5) . This polymorphism was further associated with postprandi-…”
mentioning
confidence: 99%