A polymorphic satellite sequence maps to the pericentric region of the bovine Y chromosome

Perret, Jason; Shia, Yun-Chao; Fries, R.; Vassart, Gilbert; Georges, Michel

doi:10.1016/0888-7543(90)90478-d

Cited by 47 publications

(25 citation statements)

References 18 publications

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“…The DYZ-1 marker (Perret et al, 1990;Bradley et al, 1994) was amplified using forward primer 5 0 CCT GGC GAC TGT GCA ATA TT3 0 and reverse primer 5 0 CAC ACA CAC AAC CGG TTT CT3 0 . INRA124, INRA189 and BM861 were typed as described in Edwards et al (2000).…”

Section: Laboratory Analysismentioning

confidence: 99%

Maternal and paternal genealogy of Eurasian taurine cattle (Bos taurus)

et al. 2009

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Maternally inherited mitochondrial DNA (mtDNA) has been used extensively to determine origin and diversity of taurine cattle (Bos taurus) but global surveys of paternally inherited Ychromosome diversity are lacking. Here, we provide mtDNA information on previously uncharacterised Eurasian breeds and present the most comprehensive Y-chromosomal microsatellite data on domestic cattle to date. The mitochondrial haplogroup T3 was the most frequent, whereas T4 was detected only in the Yakutian cattle from Siberia. The mtDNA data indicates that the Ukrainian and Central Asian regions are zones where hybrids between taurine and zebu (B. indicus) cattle have existed. This zebu influence appears to have subsequently spread into southern and southeastern European breeds. The most common Y-chromosomal microsatellite haplotype, termed here as H11, showed an elevated frequency in the Eurasian sample set compared with that detected in Near Eastern and Anatolian breeds. The taurine Ychromosomal microsatellite haplotypes were found to be structured in a network according to the Y-haplogroups Y1 and Y2. These data do not support the recent hypothesis on the origin of Y1 from the local European hybridization of cattle with male aurochsen. Compared with mtDNA, the intensive culling of breeding males and male-mediated crossbreeding of locally raised native breeds has accelerated loss of Ychromosomal variation in domestic cattle, and affected the contribution of genetic drift to diversity. In conclusion, to maintain diversity, breeds showing rare Y-haplotypes should be prioritised in the conservation of cattle genetic resources.

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Section: Laboratory Analysismentioning

confidence: 99%

Maternal and paternal genealogy of Eurasian taurine cattle (Bos taurus)

et al. 2009

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“…A more accurate characterization of these 3 subjects was reported a year later [Kawakura et al, 1997]. By using 2 FISH probes, BC1.2 and btDZY-3 that localized to the telomeric region [Cotinot et al, 1991] and to the centromeric region of the short arm of the Y cattle chromosome [Perret et al, 1990], respectively, the authors showed that this Y chromosome was represented by an Yp isochromosome. This condition excludes the presence of SRY , which is localized on Yq12.3 in cattle [Xiao et al, 1995;Di Meo et al, 2005].…”

Section: Xy Sex Reversal In Cattlementioning

confidence: 96%

Sex Reversal in Non-Human Placental Mammals

Parma

Veyrunes

Pailhoux

2016

Sex Dev

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Gonads are very peculiar organs given their bipotential competence. Indeed, early differentiating genital ridges evolve into either of 2 very distinct organs: the testis or the ovary. Accumulating evidence now demonstrates that both genetic pathways must repress the other in order for the organs to differentiate properly, meaning that if this repression is disrupted or attenuated, the other pathway may completely or partially be expressed, leading to disorders of sex development. Among these disorders are the cases of XY male-to-female and XX female-to-male sex reversals as well as true hermaphrodites, in which there is a discrepancy between the chromosomal and gonadal sex. Here, we review known cases of XY and XX sex reversals described in mammals, focusing mostly on domestic animals where sex reversal pathologies occur and on wild species in which deviations from the usual XX/XY system have been documented.

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“…In the PCR method, the primer pairs were synthesized from the sequence of btDYZ (upstream: CCTTTAGGG-GAAAATGGACTGACA; downstream: ATGTG-ACACTGC-TGGAAGG) reported by Perret [10] and of the bActin (upstream: ACGTCGCCTTG-GACTTCGAGCAGG; downstream: GCTGGAA-GGTGGACAGGG-AGGCCAGGA) reported by Degen [11]. The PCR mixture contained 20 µl of sample DNA (approximately 4 µg); 53.5 µl water; 8 µl 10 × PCR buffer (100 mM Tris-HCl, pH 8.3; 500 mM KCl, 15 mM MgCl 2 , 0.01% gelatin); 5 µl of each primer (20 µM); 2 µl of 10 mM dNTPs (dATP, dCTP, dTTP, dGTP); and 0.5 µl of Taq DNA polymerase (5U/µl, Perkin Elmer Cetus, Urayasu, Japan).…”

Section: Methodsmentioning

confidence: 99%

Absence of Fetal Cells in Bovine Jugular and Uterine Vein Blood at a Level of 1 in 10,000.

Kadokawa

Takusari

Minezawa

et al. 1996

Journal of Reproduction and Development

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Abstract:The objective of this study was to determine if bovine fetal cell DNA is present in pregnant maternal blood as human fetal cells are present in the blood of pregnant women after they cross the placenta and penetrate into the v. uterina. The btDYZ PCR amplification was employed to detect male-specific DNA of bovine male fetal cells. Blood samples were collected from the v. uterina and v. jugularis externa of 3 pregnant cows (2 with male fetuses and 1 with a female fetus 12 to 20 wks of age) and from only the v. jugularis externa of 33 pregnant cows (with 18 male and 15 female fetuses 10 to 40 wks of age). All blood samples were male-specific DNA negative, which indicated that fetal cells were absent in bovine maternal blood at a level of 1 in 10,000 and, therefore, cannot be used for prenatal sexing with PCR.

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A polymorphic satellite sequence maps to the pericentric region of the bovine Y chromosome

Cited by 47 publications

References 18 publications

Maternal and paternal genealogy of Eurasian taurine cattle (Bos taurus)

Maternal and paternal genealogy of Eurasian taurine cattle (Bos taurus)

Sex Reversal in Non-Human Placental Mammals

Absence of Fetal Cells in Bovine Jugular and Uterine Vein Blood at a Level of 1 in 10,000.

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