A Polymer Coating Transfer Enrichment Method for Direct Mass Spectrometry Analysis of Lipids in Biofluid Samples

Zhang, Wenpeng; Chiang, Spencer; Li, Zishuai; Chen, Qinhua; Xia, Yu; Ouyang, Zheng

doi:10.1002/anie.201900011

Cited by 32 publications

(23 citation statements)

References 53 publications

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“…In nano-ESI, the desolvation efficiency could be enhanced because of the small and monodisperse droplets, whose ionization efficiency could be increased by the higher overall charge-tovolume ratio (Wilm & Mann, 1996). Xia and Ouyang focused on locating the C=C bond for the analysis of lipids based on the coupling of a Paternò-Büchi reaction system with online nano-ESI (Ma & Xia, 2014;Ma et al, 2016;Zhang et al, 2019). Very recently, they realized large-scale and near-complete lipid characterization, in which C=C location(s) and sn-position(s) can be identified simultaneously (Cao et al, 2020).…”

Section: Homogeneous Reactionsmentioning

confidence: 99%

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Sun

Yin

et al. 2020

Mass Spectrometry Reviews

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Chemical reactions conducted in different media (liquid phase, gas phase, or surface) drive developments of versatile techniques for the detection of intermediates and prediction of reasonable reaction pathways. Without sample pretreatment, ambient mass spectrometry (AMS) has been applied to obtain structural information of reactive molecules that differ in polarity and molecular weight. Commercial ion sources (e.g., electrospray ionization, atmospheric pressure chemical ionization, and direct analysis in real-time) have been reported to monitor substrates and products by offline reaction examination. While the interception or characterization of reactive intermediates with short lifetime are still limited by the offline modes. Notably, online ionization technologies, with high tolerance to salt, buffer, and pH, can achieve direct sampling and ionization of ongoing reactions conducted in different media (e.g., liquid phase, gas phase, or surface). Therefore, short-lived intermediates could be captured at unprecedented timescales, and the reaction dynamics could be studied for mechanism examinations without sample pretreatments. In this review, via various AMS methods, chemical reaction monitoring and mechanism elucidation for different classifications of reactions have been reviewed. The developments and advances of common ionization methods for offline reaction monitoring will also be highlighted.

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Section: Homogeneous Reactionsmentioning

confidence: 99%

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Sun

Yin

et al. 2020

Mass Spectrometry Reviews

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“…40,41 Previously we had developed several direct sampling methods and miniature MS systems for the fast analysis of drugs, metabolites and lipids. [42][43][44][45][46][47][48][49] Recently, we also explored the application of miniature mass spectrometers in quantitation of synthetic peptides with molecular weights larger than 1500 Da. 50 However, due to the sophistication of sample preparation procedures, fast analysis of proteins and digested peptides by miniature MS is still difficult.…”

Section: Application In the Analysis Of Protein Biomarkers Of Mycobacterium Tuberculosismentioning

confidence: 99%

Fast protein analysis enabled by high-temperature hydrolysis

2020

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“…One of the most representative applications is a photochemical (Paternò–Büchi) reaction for fast identification of lipid CC location. Xia's group developed the fast derivatization reaction for lipid CC with acetone, and applied this for online derivatization of lipids in biofluids and tissues . For instance, the biofluid sample was pushed into a capillary preloaded with 5 µL acetone/water, and online reaction was then performed under UV exposure by a low power mercury lamp at 254 nm.…”

Section: Ms Analysis Of Clinical Biomoleculesmentioning

confidence: 99%

Mass Spectrometry Methods for In Situ Analysis of Clinical Biomolecules

Liu

et al. 2019

Small Methods

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past few decades, great efforts and substantial progress have been made in this field, and various methods, such as colorimetric screening, [3] enzyme-linked immunosorbent assay (ELISA), [4] fluorescence, [5] polymerase chain reaction (PCR), [6] and electrochemical assays, [7] have been extensively performed in clinical laboratory tests. Although with efficiency and robustness, the majority of these methods still lack sufficient accuracy, sensitivity, and specificity for clinical diagnostic applications.The inevitable complexity and challenges of clinical biomolecules detection have encouraged researchers to continuously devote great effort to the development of new methods with accuracy, rapidity, and simplicity. Relying on its high throughput and accuracy, great sensitivity and versatility, reliable qualitative and quantitative ability, and multiplexed detection capability, mass spectrometry (MS) is exceptionally suitable for clinical analysis and blooming in modern healthcare industry in recent years. [8][9][10] MS coupled to liquid chromatography (LC) and gas chromatography (GC) is regarded as gold standards for quantitative analysis of various compounds and have been conventionally used in clinical applications such as toxicology, [11] therapeutic drug monitoring, [12] inborn error of metabolism (IEM), [13] and steroid hormone testing [14] for decades. IEMs cause high morbidity and mortality in children, and the utilization of GC-MS and LC-MS expands newborn IEMs screening from single-screening assays to simultaneously screening of various analytes (e.g., amino acids, organic acids, carnitine, and acylcarnitine) in a single run using one sample from the patient. Some reviews have summarized a number of excellent works about applications of GC-MS and LC-MS in clinical chemistry. [15,16] These hyphenated MS methods provide accurate quantification data and abundant information of clinical biomolecules, which facilitates biological behavior understanding, biomarker discovery, and prognosis evaluation. However, in most cases, these methods are conducted by using lysis of cells or tissues, which fail to provide real-time information and spatial distribution of biomolecules in their native states. On the other hand, tedious sample pretreatment (separation, purification, and redissolution) is inevitable. These series of labor intensive and time-consuming procedures may cause unpredictable sample loss and undervaluation of biomolecules. Therefore, chromatography-based MS methods are limited to achieve high-throughput, in situ, and visual clinical applications. The development of emerging Due to the significant role of clinical biomolecules in mediating biological activities and disease progressions, in situ analysis provides a great opportunity for understanding the molecular mechanisms of biological functions, facilitating medical diagnosis, clinical treatment, and prognosis. Among all the analysis methods, mass spectrometry (MS) methodologies exhibit unique features that make them exceptionally suitable for clinica...

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A Polymer Coating Transfer Enrichment Method for Direct Mass Spectrometry Analysis of Lipids in Biofluid Samples

Cited by 32 publications

References 53 publications

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Fast protein analysis enabled by high-temperature hydrolysis

Mass Spectrometry Methods for In Situ Analysis of Clinical Biomolecules

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