A Polymer Blend Substrate for Skeletal Muscle Cells Alignment and Photostimulation

Vurro, Vito; Scaccabarozzi, Albaerto Davide; Lodola, Francesco; Storti, Filippo; Marangi, Fabio; Ross, Aaron M.; Paternò, Giuseppe M.; Scotognella, Francesco; Criante, Luigino; Caironi, Mario; Lanzani, Guglielmo

doi:10.1002/adpr.202000103

Cited by 10 publications

(10 citation statements)

References 70 publications

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“…Capacitance measurements were performed as previously described. 49 , 61 Briefly, a double sinusoidal voltage clamp signal was applied to the cell in whole-cell configuration. The response current signal was acquired, and membrane capacitance and resistance were extracted fitting the current with a custom MATLAB program.…”

Section: Methodsmentioning

confidence: 99%

Optical modulation of excitation-contraction coupling in human-induced pluripotent stem cell-derived cardiomyocytes

Vurro¹,

Federici²,

Ronchi³

et al. 2023

iScience

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Section: Methodsmentioning

confidence: 99%

Optical modulation of excitation-contraction coupling in human-induced pluripotent stem cell-derived cardiomyocytes

Vurro¹,

Federici²,

Ronchi³

et al. 2023

iScience

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“…Capacitance measurements were performed as previously described (Vurro et al, 2020, 2021). Briefly, a double sinusoidal voltage-clamp signal was applied to the cell in whole-cell configuration.…”

Section: Methodsmentioning

confidence: 99%

“…Capacitance measurements were performed as previously described (Vurro et al, 2020(Vurro et al, , 2021.…”

Section: Capacitance Recordingsmentioning

confidence: 99%

Optical modulation of excitation-contraction coupling in human induced pluripotent stem cell-derived cardiomyocytes

Vurro

Federici

Ronchi

et al. 2022

Preprint

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Non-genetic photostimulation is a novel and rapidly growing multidisciplinary field of research that aims to induce light sensitivity in living systems by exploiting exogeneous phototransducers. Here we propose a recently synthetized intramembrane photo-transducer, based on an azobenzene derivative (Ziapin2), for optical pacing of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The light-mediated photostimulation process has been studied by applying a number of characterization techniques to detect the effect on the cell properties. In particular, we recorded changes in membrane capacitance, in membrane potential (Vm), and modulation of intracellular Ca2+ dynamics. Finally, cell contractility was analyzed using a custom MATLAB algorithm. Photostimulation of intra-membrane Ziapin2 causes a transient Vm hyperpolarization followed by a delayed depolarization and action potential firing. The observed initial electrical modulation nicely correlates with changes in Ca2+ dynamics and contraction rate. This work represents the proof of principle that Ziapin2 is able to modulate electrical activity and contractility in hiPSC-CMs, opening up a future development in cardiac electrophysiology.

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“…The use of organic electronic devices in bioelectronics has seen a steep rise in the last couple of decades. − One of the most important reasons is that organic semiconductors share many common features with biological matter, such as softness (i.e., in terms of supramolecular interaction and charge transport) and mixed ionic–electronic conduction . In addition, these materials exhibit superior biocompatibility, conformability, and cost-effectiveness compared to their inorganic counterparts.…”

Section: Introductionmentioning

confidence: 99%

Resonant Enhancement of Polymer–Cell Optostimulation by a Plasmonic Metasurface

et al. 2022

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Organic semiconductors have shown great potential as efficient bioelectronic materials. Specifically, photovoltaic polymers such as the workhorse poly(thiophene) derivatives, when stimulated with visible light, can depolarize neurons and generate action potentials, an effect that has been also employed for rescuing vision in blind rats. In this context, however, the coupling of such materials with optically resonant structures to enhance those photodriven biological effects is still in its infancy. Here, we employ the optical coupling between a nanostructured metasurface and poly(3-hexylthiophene) (P3HT) to improve the bioelectronic effects occurring upon photostimulation at the abiotic−biotic interface. In particular, we designed a spectrally tuned aluminum metasurface that can resonate with P3HT, hence augmenting the effective field experienced by the polymer. In turn, this leads to an 8-fold increase in invoked inward current in cells. This enhanced activation strategy could be useful to increase the effectiveness of P3HT-based prosthetic implants for degenerative retinal disorders.

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A Polymer Blend Substrate for Skeletal Muscle Cells Alignment and Photostimulation

Cited by 10 publications

References 70 publications

Optical modulation of excitation-contraction coupling in human-induced pluripotent stem cell-derived cardiomyocytes

Optical modulation of excitation-contraction coupling in human-induced pluripotent stem cell-derived cardiomyocytes

Optical modulation of excitation-contraction coupling in human induced pluripotent stem cell-derived cardiomyocytes

Resonant Enhancement of Polymer–Cell Optostimulation by a Plasmonic Metasurface

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