A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein

Lü, Ming; Cao, Chenyang; Wang, Zewei; Liu, Guocong

doi:10.1016/j.matdes.2020.109409

Cited by 34 publications

(12 citation statements)

References 34 publications

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“…During the construction of the CAP aptasensor, the EIS characterization of various modified electrodes is shown in Figure 2 B, which exhibits typical Nyquist plots of bare GCE (curve a), MXene–AuNP/GCE (curve b), Apt/MXene-AuNP/GCE (curve c), MCH/Apt/MXene-AuNP/GCE (curve d) and CAP/MCH/Apt/MXene-AuNP/GCE (curve e) in [Fe (CN) 6 ] 3−/4− mixture solution (1:1) containing 0.1 M KCl. In the EIS, the higher-frequency semicircle corresponds to the electron-transfer-limited process, and its diameter is equal to the electron-transfer resistance [ 18 ]. A semicircle with smaller resistance value was observed at MXene–AuNP/GCE (curve b) than the bare GCE (curve a).…”

Section: Resultsmentioning

confidence: 99%

“…The sequence of CAP-binding aptamer (Apt) was obtained from Sangon Biotechnology Co., Ltd. (Shanghai, China), and its sequence is as follows: 5′-C6/SH-ACT TCA GTG AGT TGT CCC ACG GTCGGC GAG TCG GTG GTA G-3′, which was dissolved in 1 × TE buffer (pH = 7.6, 10 mM Tris-HCl, 1 mM EDTA) to attain stock solutions with a certain concentration [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…In the construction of electrochemical biosensors, the use of various functional materials is a common method for signal amplification since appropriate nanomaterial-modified sensing interfaces can effectively improve detection performance [ 17 ]. Till now, several nanomaterial-based electrochemical aptasensors, such as polyethyleneimine-reduced graphene oxide/gold nanocubes [ 18 ], Ag nanoparticle-sensitized bismuth oxyiodide [ 19 ], and gold nanoparticle-functionalized 3D flower-like MoS 2 /TiO 2 [ 20 ], have been reported for CAP detection. Nevertheless, the sensitivity of these sensors still needs to be improved.…”

Section: Introductionmentioning

confidence: 99%

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MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

Yang

Zhong

et al. 2022

Molecules

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A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene–AuNP modified electrode surface through Au–S interaction. Upon specifically binding with CAP with high affinity, the CAP–Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001–10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

Yang

Zhong

et al. 2022

Molecules

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“…Additional amplification has been demonstrated by treating the capturing DNA remained after the aptamer release with Recjf exonuclease that cleaves the DNA sequence and results in further decreases in the resistance of the charge transfer [ 264 ]. The opposite effect can be achieved by the reaction of auxiliary DNA with protein specifically bonded to single-stranded DNAs [ 245 , 263 ]. The indirect competitive assay is realized in [ 246 ].…”

Section: Electrochemical Aptasensors For Antibiotic Detectionmentioning

confidence: 99%

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

Evtugyn

Porfireva

Tsekenis

et al. 2022

Sensors

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Antibiotics are often used in human and veterinary medicine for the treatment of bacterial diseases. However, extensive use of antibiotics in agriculture can result in the contamination of common food staples such as milk. Consumption of contaminated products can cause serious illness and a rise in antibiotic resistance. Conventional methods of antibiotics detection such are microbiological assays chromatographic and mass spectroscopy methods are sensitive; however, they require qualified personnel, expensive instruments, and sample pretreatment. Biosensor technology can overcome these drawbacks. This review is focused on the recent achievements in the electrochemical biosensors based on nucleic acid aptamers for antibiotic detection. A brief explanation of conventional methods of antibiotic detection is also provided. The methods of the aptamer selection are explained, together with the approach used for the improvement of aptamer affinity by post-SELEX modification and computer modeling. The substantial focus of this review is on the explanation of the principles of the electrochemical detection of antibiotics by aptasensors and on recent achievements in the development of electrochemical aptasensors. The current trends and problems in practical applications of aptasensors are also discussed.

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“…Traditional amplification strategies include labeling amplification, where a single nanomaterial-based tag incorporates numerous signal-generation elements, and target amplification, where a single target generates many signaling molecules through a multitude of analyte-recognition events [ 120 ]. Numerous aptasensing studies focused on food contaminants have employed the first strategy, based on nanomaterials, such as carbon nanomaterials (CNs) [ 28 , 96 , 124 , 125 ] and metal nanoparticles [ 125 , 126 , 127 ], or enzyme labeling [ 69 , 79 ]. The second signal amplification strategy aims to increase the signal/target concentration ratio, as in the case of nuclease-based target recycling [ 127 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

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Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

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A polyethyleneimine reduced graphene oxide/gold nanocubes based electrochemical aptasensor for chloramphenicol detection using single-stranded DNA-binding protein

Cited by 34 publications

References 34 publications

MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

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