A polydimethylsiloxane/glass capillary electrophoresis microchip for the analysis of biogenic amines using indirect fluorescence detection

Abstract

A polydimethylsiloxane-glass capillary microchip is fabricated for the rapid analysis of a mixture of common biogenic amines using indirect fluorescence detection. Using a running buffer of phosphate and 2-propanol, and Rhodamine 110 as a background fluorophore, both co-ionic and counter-ionic systems are explored. Studies demonstrate the separation and analysis of cations using indirect fluorescence detection for the first time in a chip-based system. Resulting electrophoretic separations are achieved within a few tens of seconds with detection limits of approximately 6 microM. The reduced sample handling and rapid separations afforded by the coupling of indirect fluorescence detection with chip-based capillary electrophoresis provide a highly efficient method for the analysis and detection of molecules not possessing a chromophore or fluorophore. Furthermore, limits of detection are on a par with reported chip-based protocols that incorporate precolumn derivatisation with fluorescence detection. The current device circumvents lengthy sample preparation stages and therefore provides an attractive alternative technique for the analysis biogenic amines.

Application of microfluidic chip with integrated optics for electrophoretic separations of proteins☆

“…Exemplary devices were fabricated in full glass [23][24][25][26], polycarbonate [27], double layer PDMS [28], poly(methyl methacrylate) (PMMA) [29,30]. Although PDMS channels have been already described in PDMS/glass device [31][32][33][34][35][36], no glass channels have been reported in such hybrid device.…”

Optical fibre-based micro-analyser for indirect measurements of volatile amines levels in fish

“…Many analytical methods have been developed for the quantification of volatile amines, including TMA and DMA, in seafood products, based on: colorimetry (AOAC, 1995); potentiometric detection (Adhoum et al, 2003;Dhaouadi, Monser, Sadok, & Adhoum, 2007); high performance liquid chromatography (HPLC) coupled with UV detectors (Alberto, Arena, & Nadra, 2002;Kaniou, Samouris, Mouratidou, Eleftheriadou, & Zantopoulos, 2001); gas chromatography-flame ionisation detection (GC-FID) (Chien, Uang, Kuo, Shih, & Jen, 2000;Fiddler, Doerr, & Gates, 1991;Li et al, 2004;Veciana-Nogues, Albala-Hurtado, Izquierdo-Pulido, & Vida-Carou, 1996); gas chromatography-mass spectrometry (GC-MS) (Mills, Walker, & Mughal, 1999); ion mobility spectrometry (IMS) (Karpas, Tilman, Gdalevsky, & Lorber, 2002); capillary electrophoresis (CE) with UV and fluorescence detection (Beard & Mello, 2002;Timm & Jorgensen, 2002); metalloporphyrins-coated quartz microbalance sensor array (electronic nose) (Ampuero, Zesiger, Gustafsson, Lunden, & Bosset, 2002); semi-conducting metal oxides sensors (Hammond et al, 2002); colorimetric sensor (Pacquit et al, 2006) and selective electrode (Mitsubayashi et al, 2004).…”

Direct analysis of biogenic amines in water matrix by modified capillary zone electrophoresis with 18-crown-6

“…Comparing with detection limit values obtained with different analytical techniques described in the literatures, the CZE-AD method showed improved sensitivity (~10 ng.mL −1 vs~1 μM) in relation to the detection limits obtained in reported microchip CE with indirect fluorimetric detection [23], CE with fluorimetric detection [24], conductometric detection [29] and contactless conductivity detection [30] methods, and was comparable (~10 ng.mL −1 vs~100 nM) to those achieved in HPLC with diode array detection [15] and fluorimetric detection [16], CE with pulsed AD [27], and MECC with electrochemical detection [28] approaches. In addition, most of these reported methods rely on complicated derivatization procedure or valuable instrumentation.…”

“…However, the detection of BAs is rather difficult due to their low volatility and lack of chromophores, and then derivatization procedure (pre-or post-column) has been usually applied. To prevent problems such as long reaction time, tedious processes, or side products associated with derivatization, a number of indirect detection methods have been reported [20][21][22][23][24]. Indirect UV-vis absorption and indirect laser-induced fluorescence (LIF) are the two most common indirect detection methods in CE [18,25].…”