A polyacrylamide gel containing an engineered hexameric hemoprotein as a cross-linking unit toward redox-responsive materials

Kageyama, Kazuki; Oohora, Koji; Hayashi, Takashi

doi:10.1039/d3ra05897b

RSC Adv.

2023

DOI: 10.1039/d3ra05897b

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A polyacrylamide gel containing an engineered hexameric hemoprotein as a cross-linking unit toward redox-responsive materials

Kazuki Kageyama,

Koji Oohora,

Takashi Hayashi

Abstract: We demonstrate fabricating a redox-responsive polyacrylamide gel using a hexameric hemoprotein reconstituted with acryloyl group-tethered heme derivatives.

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2023

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Elasticity tuning of a hexameric hemoprotein-based hydrogel by mutation of its protein building block

Oohora,

Kageyama,

Hidaka

et al. 2023

Chemistry Letters

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Mutants of a hexameric hemoprotein reconstituted with an acryloyl group-tethered heme were prepared and used as a cross-linker to produce polyacrylamide hydrogels to evaluate the effect of mutations on their elastic modulus. The mutations decrease the heme-binding affinities in a solution state, reducing the Young’s modulus by 30% relative to that of the wild-type protein-based hydrogel. Furthermore, a stimuli-responsive gel-sol transition occurs upon reduction of the mutant-based gel. This work indicates that the molecular-level heme affinity for the cross-linked protein units has an important influence on macro-level mechanical properties.

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Elasticity tuning of a hexameric hemoprotein-based hydrogel by mutation of its protein building block

Oohora,

Kageyama,

Hidaka

et al. 2023

Chemistry Letters

View full text Add to dashboard Cite

show abstract

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A polyacrylamide gel containing an engineered hexameric hemoprotein as a cross-linking unit toward redox-responsive materials

Abstract: We demonstrate fabricating a redox-responsive polyacrylamide gel using a hexameric hemoprotein reconstituted with acryloyl group-tethered heme derivatives.

Cited by 1 publication

References 78 publications

Elasticity tuning of a hexameric hemoprotein-based hydrogel by mutation of its protein building block

Elasticity tuning of a hexameric hemoprotein-based hydrogel by mutation of its protein building block

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