A poly(lactide‐co‐glycolide) film loaded with abundant bone morphogenetic protein‐2: A substrate‐promoting osteoblast attachment, proliferation, and differentiation in bone tissue engineering

Abstract

We explored a novel biodegradable poly(lactide-co-glycolide) (PLGA) film loaded with over 80 wt % bone morphogenetic protein (BMP)-2, which was regarded as a substrate-promoting osteoblast attachment, proliferation, and differentiation for application of bone tissue engineering. Using phospholipid as a surfactant, BMP-2 was modified as a complex (PBC) for dispersing in PLGA/dichloromethane solution. The PLGA film loaded with BMP-2 and phospholipid complex (PBC-PF) showed rough and draped morphology with high entrapment efficiency exceeding 80% and good hydrophilicity, respectively. The in vitro release study of BMP-2 showed that about 50% BMP-2 was slowly and continuously released from PBC-PF within 5 weeks and had a short initial burst release only in the last 1.5 days, which was better than serious burst release of PLGA film loaded with pure BMP-2 without phospholipid (BMP-PF) as control. By comparison with other PLGA films and tissue culture plates, it was confirmed that PBC-PF significantly promoted the attachment, proliferation, and differentiation of osteoblasts with higher entrapment efficiency and better sustained release. These advantages illustrated that PBC-PF could be a potential substrate providing long-term requisite growth factors for osteoblasts, which might be applied in bone tissue engineering.

Soybean Lecithin‐Mediated Nanoporous PLGA Microspheres with Highly Entrapped and Controlled Released BMP‐2 as a Stem Cell Platform

“…To investigate differentiation and matrix mineralization of hBMSCs on PLGA‐based microspheres, the expression levels of osteogenic genes at 15 d after cell seeding were estimated by qRT‐PCR analysis . Using the QuantiTect SYBR Green PCR kit (Qiagen, Hilden, Germany), we analyzed six kinds of gene markers: COL‐1, MGP, RUNX2, OCN, OPN, and OPG.…”

Crystallization Behavior of Poly(p-dioxanone-co-glycolide) Copolymers

“…T g,A was 257 K, the T g of PPDO , and T g,B was 309 K, the T g of PGA, respectively [9,10]. The results showed that both T g testing value and T g calculation value of PDG were in a linear relation with percent of GA and both of value fell in between T g of PPDO and that of PGA (Fig.2).…”

Combined antibacterial and osteogenic in situ effects of a bifunctional titanium alloy with nanoscale hydroxyapatite coating

“…To investigate the osteogenic differentiation of the hBMSCs on the Ti specimens, the alkaline phosphatase (ALP) activity and the total protein content of the cells were measured using an ALP Activity Kit and a Protein Assay Kit (both from Invitrogen, USA), respectively, as reported previously [23]. Furthermore, the expression levels of osteogenic genes at 7 days after cell seeding were determined by quantitative real-time polymerization chain reaction (PCR) analysis [24] using the QuantiTect SYBR Green PCR Kit (Qiagen, Hilden, Germany), and primers specific for six gene markers: collagen type-1 (COL-1), osteocalcin (OCN), osteopontin (OPN), osteoprotegerin (OPG), runt-related transcription factor 2 (RUNX2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; internal control for mRNAs) (Supplemental data, Table S1).…”