A poly(l-lactic acid) nanofibre mesh scaffold for endothelial cells on vascular prostheses

François, Sébastien; Chakfé, Nabil; Durand, Bernard; Laroche, Gaétan

doi:10.1016/j.actbio.2009.03.013

Cited by 44 publications

(52 citation statements)

References 20 publications

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“…Similarly, in our study, the endothelial cell growth was improved after functionalization of PLG and PLGC with cell-adhesive protein multilayers. In another study, the proliferation rate of bovine aortic EC was compared on commercial textile PET prostheses and on prostheses where industrial Co impregnation was experimentally replaced by an air-spun poly (L-lactic acid) (PLLA) nanofiber mesh [9]. The authors found increased cell proliferation on commercial grafts until day 3; however, from day 5 to day 15 the cell growth was significantly better on the PET-PLLA.…”

Section: Discussionmentioning

confidence: 99%

“…Their luminal surface was lined with a layer of endothelial cells with myofibroblasts, fibroblasts and collagen underneath [15]. Similarly, when the inner surface of PET prostheses was coated with PLLA nanofibers, these grafts displayed a significantly better proliferation rate of endothelial cells and formation of endothelial cell monolayer than uncoated PET prostheses [9].…”

Section: Introductionmentioning

confidence: 88%

“…Nevertheless, firm mechanical reinforcement ensures superior safety by preventing potential graft rupture or aneurysm formation [15]. In addition, degradable polymers may be associated with increased local environment acidity [18]; however, degradation products would be washed out in vascular dynamic conditions, and the advantage of replacing the polymer step-by-step by cell-secreted matrix is selfevident [9].…”

Section: Discussionmentioning

confidence: 99%

“…The authors found increased cell proliferation on commercial grafts until day 3; however, from day 5 to day 15 the cell growth was significantly better on the PET-PLLA. They declare that PET sprayed with PLLA provides a nano-featured and more suitable 2-D environment for EC spreading than 3-D commercial PET, which induces cell clustering within the textile matrix [9]. It is known that nanostructured substrates promote the adhesion and growth of cells by supporting physiological conformation of the cell adhesionmediating molecules (e.g., fibronectin, vitronectin), which are spontaneously adsorbed to these substrates from biological fluids including cell culture media [7].…”

Section: Discussionmentioning

confidence: 99%

“…However, after fulfilling the haemostatic role, the healing incorporation of the impregnated prosthesis was delayed for several weeks to months due to resorption of the matrix, depending on the type of protein and cross-linking agent. Moreover, the impregnated prostheses were not optimal substrata for direct endothelialization, because collagen impregnation is derived from fragmented biological tissue that is not created to optimize endothelial proliferation [8,9]. In addition, collagen as a component of bio-prostheses has often been cross-linked by glutaraldehyde and related agents, which are cytotoxic and may cause calcification of the grafts [10].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Filova¹

2014

J Tissue Sci Eng

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Filova¹

2014

J Tissue Sci Eng

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Enzymatic degradation of polylactide/layered silicate nanocomposites: Effect of organic modifiers

Singh

Purkayastha

Panigrahi

et al. 2012

J of Applied Polymer Sci

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Biodegradable polylactide (PLA)/layered silicate nanocomposites have been prepared via solution route using two different kinds of organically modified nanoclays. The nanostructure as observed from wide-angle X-ray diffraction indicates intercalated hybrids and the extent of intercalation depends on the type of organic modifiers used. Melt-quenched PLA and its nanocomposites are predominantly amorphous but, after annealing, they are fairly crystalline. The nanohybrids show significant improvement in thermal properties as compared to neat polymer. The nature of interaction between nanoclays and matrix polymer depends on the organic modifiers used, as evident from varying heat of fusion and shifting of Fourier transform infrared peaks. The nanoclays act as nucleating agent, and thereby, control the spherulite dimension of the matrix. The comparison of biodegradation of PLA and its nanocomposites has been studied in enzyme, compost, and buffer solution. Biodegradability of PLA has significantly been enhanced in the presence of nanoclays and the rate varies on organic modifications. The surface morphology, before and after enzymatic degradation, confirms the relative rate of degradation through laser scanning confocal images, scanning electron microscope, and atomic force microscope.

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Characterization of an air‐spun poly(L‐lactic acid) nanofiber mesh

et al. 2010

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It was previously showed that PLLA nanofiber mesh promoted good endothelial cell proliferation. A new technique was developed to produce nanofibers by air jet spinning inside the tubular shape of vascular prostheses and to characterize this nanofiber mesh. Polymer macromolecule stability was assessed by gel permeation chromatography. Thermal analyses were conducted with differential scanning calorimetry and dynamic mechanical analysis on PLLA nanofibers obtained with 4% and 7% solutions (w/v) in chloroform. Polyethylene terephthalate (PET) was also treated with atmospheric pressure dielectric barrier discharge under air or nitrogen atmosphere to optimize PLLA nanofiber adherence, assessed by peel tests. Air spinning induced a reduction of number-average molecular weight (M(n)) for the 7% PLLA solution but not for the 4% solution. The nanofibers were more crystalline and less sensible to viscoelastic relaxation as a function of aging in the 4% solution than in the 7% solution. Discharge treatment of the PET promoted identical surface modification on PET film and PET textile surfaces. Moreover, the best PLLA nanofibers adhesion results were obtained under nitrogen atmosphere. This study demonstrates that it is possible to coat the internal side of tubular vascular prostheses with PLLA nanofibers, and provides a better understanding of the air spinning process as well as optimizing nanofibers adhesion.

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A poly(l-lactic acid) nanofibre mesh scaffold for endothelial cells on vascular prostheses

Cited by 44 publications

References 20 publications

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Enzymatic degradation of polylactide/layered silicate nanocomposites: Effect of organic modifiers

Characterization of an air‐spun poly(L‐lactic acid) nanofiber mesh

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