A poloxamer-polypeptide thermosensitive hydrogel as a cell scaffold and sustained release depot

Lin, Ji-Yu; Lai, Po‐Liang; Lin, Yi; Peng, Sydney; Lee, Li-Yu; Chen, Chieh-Nan; Chu, I‐Ming

doi:10.1039/c5py02067k

Cited by 26 publications

(24 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The copolymer synthesis was similar to that in our previous study conducted in 2016 [14] in which l -alanine (5.0 g and 56.1 mmol) and triphosgene (16.65 g, 56.3 mmol) were dissolved in anhydrous THF (150 mL) and gently stirred at 45 °C under nitrogen flux. After the reaction period of approximately 12 h, the mixture turned clear and the solution was condensed to a final volume of 15 mL through rotary evaporation.…”

Section: Methodsmentioning

confidence: 73%

“…As in Figure 1B, peaks at 3300 cm −1 (N–H bond) and 1600–1720 cm −1 (amide bond) are from polypeptides, while those found at 2900 cm −1 (C–H) and 1100 cm −1 (C–O) are from the PLX segment. The characterization and structure of the triblock copolymer P–Lys–Ala–PLX is explained in our 2016 study [14].…”

Section: Resultsmentioning

confidence: 99%

“…The gelling process involves a reduced hydrogen bond interaction between the hydrophilic segments of polymers and water as the temperature rises [10,11]. Different types of hydrophilic polymers are used in this gelling process, including various hydrogels, poly(ethylene glycol) (PEG) [12], methoxy poly(ethylene glycol) (mPEG) [13], and PEO–PPO–PEO copolymers (mainly the Pluronic ® or poloxamer (PLX) series) [14]. Several types of polypeptides have been used in the hydrophobic portion of the polymer [12,15,16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Mixed Thermosensitive Hydrogel System for Sustained Delivery of Tacrolimus for Immunosuppressive Therapy

et al. 2019

Self Cite

View full text Add to dashboard Cite

Tacrolimus is an immunosuppressive agent for acute rejection after allotransplantation. However, the low aqueous solubility of tacrolimus poses difficulties in formulating an injection dosage. Polypeptide thermosensitive hydrogels can maintain a sustained release depot to deliver tacrolimus. The copolymers, which consist of poloxamer and poly(l-alanine) with l-lysine segments at both ends (P–Lys–Ala–PLX), are able to carry tacrolimus in an in situ gelled form with acceptable biocompatibility, biodegradability, and low gelling concentrations from 3 to 7 wt %. By adding Pluronic F-127 to formulate a mixed hydrogel system, the drug release rate can be adjusted to maintain suitable drug levels in animals with transplants. Under this formulation, the in vitro release of tacrolimus was stable for more than 100 days, while in vivo release of tacrolimus in mouse model showed that rejection from skin allotransplantation was prevented for at least three weeks with one single administration. Using these mixed hydrogel systems for sustaining delivery of tacrolimus demonstrates advancement in immunosuppressive therapy.

show abstract

Section: Methodsmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Mixed Thermosensitive Hydrogel System for Sustained Delivery of Tacrolimus for Immunosuppressive Therapy

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nanogels can also be designed to release drugs under exogenous stimuli, especially temperature . When temperature changes, the sizes of some nanogels change along with the phase transition of a particular block, leading to the controlled release of drugs from these nanogels …”

Section: Monoresponsive Polypeptide Nanogelsmentioning

confidence: 99%

Advances in Stimuli‐Responsive Polypeptide Nanogels

et al. 2018

View full text Add to dashboard Cite

Polymer nanogels, which are nanosized particles of hydrogels formed by physically or chemically crosslinking polymers, are one of the most promising nanoplatforms for use in biomedical applications, including drug delivery, gene therapy, and bioimaging. Polypeptide nanogels exhibit many advantages for applications in biomedical fields, including stable 3D structures, high drug‐loading efficiency, excellent biocompatibility, stimuli responsiveness, and so forth. More specifically, smart polypeptide nanogels undergo suitable transitions under endogenous (e.g., reduction, reactive oxygen species, pH, and enzymes) or exogenous stimuli (e.g., light, temperature, voltage, and magnetic fields) for on‐demand drug delivery. Here, a comprehensive introduction to the preparation and applications of diverse stimuli‐responsive polypeptide nanogels is given. Moreover, the opportunities and challenges of polypeptide nanogels for the development of biomedicine are briefly predicted.

show abstract

“…In addition, polypeptide combined with PEG or Pluronic exhibited thermo‐sensitive behavior mainly attributed to the dehydration of PEG as well as the secondary structure of polypeptide. There have been extensive studies on block copolymers of PEG and polyalanine, polyglutamate derivates, polytyrosine, or block copolymers of Pluronic and poly(alanine‐ co ‐leucine), poly(alanine‐ co ‐lysine) . Moreover, EG oligomers as side chains for poly( l ‐glutamate) (poly( l ‐EG x Glu)) has been synthesized by Li et al, who also designed hydrogels by incorporation of poly( l ‐EG 2 Glu) with PEG.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and gelation of copolypept(o)ides with random and block structure

et al. 2017

View full text Add to dashboard Cite

Copolypept(o)ides of polysarcosine (PSar) and poly(N‐isopropyl‐L‐glutamine) (PIGA) with random and block sequence structures were synthesized by ring‐opening polymerization (ROP) of sarcosine N‐carboxyanhydrides (Sar‐NCA) and γ‐benzyl‐l‐glutamate N‐carboxyanhydrides (BLG‐NCA) and post modification. With different distribution of Sar along the main chain, H‐bonding pattern and secondary structure of polypeptides were turned, as well as aggregation and gelation behavior. Both copolypept(o)ides formed hydrogels above their critical gelation concentrations (CGCs) without thermo‐sensitivity, which was normally reserved for PEG copolypeptides (eg, PEG‐b‐PIGA). In particular, a different mechanism from previously reported micellar percolation or fibrillar entanglement was suggested for gelation of the random copolypept(o)ide. Therefore, hydrogels from copolymers of PSar and PIGA represented a new approach to construct easy‐handling, biocompatible, biodegradable and thermo‐stable gels that could potentially be applied in biomedical fields.

show abstract

A poloxamer-polypeptide thermosensitive hydrogel as a cell scaffold and sustained release depot

Abstract: Herein, we report the synthesis and characterization of a novel positively charged thermosensitive hydrogel prepared from poloxamer (PLX)-poly(l-alanine-lysine) (Lys-Ala-PLX-Ala-Lys) that demonstrates potential in biomedical applications including tissue engineering and drug delivery.

Cited by 26 publications

References 34 publications

A Mixed Thermosensitive Hydrogel System for Sustained Delivery of Tacrolimus for Immunosuppressive Therapy

A Mixed Thermosensitive Hydrogel System for Sustained Delivery of Tacrolimus for Immunosuppressive Therapy

Advances in Stimuli‐Responsive Polypeptide Nanogels

Synthesis and gelation of copolypept(o)ides with random and block structure

Contact Info

Product

Resources

About