A poke into the diversity and associations within human anterior nare microbial communities

Wos‐Oxley, Melissa L.; Plumeier, Iris; Eiff, Christof von; Taudien, Stefan; Platzer, Matthias; Vílchez-Vargas, Ramiro; Becker, Karsten; Pieper, Dietmar H.

doi:10.1038/ismej.2010.15

Cited by 105 publications

(129 citation statements)

References 40 publications

Supporting

Mentioning

122

Contrasting

Unclassified

Order By: Relevance

“…We found an abundance of skin lineages as reported in previous molecular studies of the anterior nare and nostril including Staphylococcaceae, Propionbibacteriaceae, and Corynebacteriaceae (13,(31)(32)(33)(34). In addition, NP samples contained organisms commonly found in the oral cavity, such as Streptococcaceae, Veillonellaceae, and Prevotellaceae (11,13,14,32).…”

Section: Bacterial Community Composition Analyzed By 454 Pyrosequencingsupporting

confidence: 51%

Topographical Continuity of Bacterial Populations in the Healthy Human Respiratory Tract

Charlson

Bittinger²,

Haas

et al. 2011

Am J Respir Crit Care Med

908

930

View full text Add to dashboard Cite

Rationale: Defining the biogeography of bacterial populations in human body habitats is a high priority for understanding microbial-host relationships in health and disease. The healthy lung was traditionally considered sterile, but this notion has been challenged by emerging molecular approaches that enable comprehensive examination of microbial communities. However, studies of the lung are challenging due to difficulties in working with low biomass samples. Objectives: Our goal was to use molecular methods to define the bacterial microbiota present in the lungs of healthy individuals and assess its relationship to upper airway populations. Methods: We sampled respiratory flora intensively at multiple sites in six healthy individuals. The upper tract was sampled by oral wash and oro-/nasopharyngeal swabs. Two bronchoscopes were used to collect samples up to the glottis, followed by serial bronchoalveolar lavage and lower airway protected brush. Bacterial abundance and composition were analyzed by 16S rDNA Q-PCR and deep sequencing. Measurements and Main Results: Bacterial communities from the lung displayed composition indistinguishable from the upper airways, but were 2 to 4 logs lower in biomass. Lung-specific sequences were rare and not shared among individuals. There was no unique lung microbiome. Conclusions: In contrast to other organ systems, the respiratory tract harbors a homogenous microbiota that decreases in biomass from upper to lower tract. The healthy lung does not contain a consistent distinct microbiome, but instead contains low levels of bacterial sequences largely indistinguishable from upper respiratory flora. These findings establish baseline data for healthy subjects and sampling approaches for sequence-based analysis of diseases.

show abstract

Section: Bacterial Community Composition Analyzed By 454 Pyrosequencingsupporting

confidence: 51%

Topographical Continuity of Bacterial Populations in the Healthy Human Respiratory Tract

Charlson

Bittinger²,

Haas

et al. 2011

Am J Respir Crit Care Med

908

930

View full text Add to dashboard Cite

show abstract

“…For bacteria, the primers 27F (5=-AGAGTTTGATCMTGGCTCAG-3=) and 521R (5=-ACCGTGGCTGCTGGCAC-3=) were used to amplify parts of the 16S rRNA gene (17), including the variable regions V1 to V3 of the small subunit of the ribosome (SSU), with an amplicon size between 459 bp for Nocardia sp. and 505 bp for Veillonella sp.…”

Section: Methodsmentioning

confidence: 99%

Cohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements

et al. 2015

View full text Add to dashboard Cite

The respiratory mycobiome is an important but understudied component of the human microbiota. Like bacteria, fungi can cause severe lung diseases, but their infection rates are much lower. This study compared the bacterial and fungal communities of sputum samples from a large cohort of 56 adult patients with cystic fibrosis (CF) during nonexacerbation periods and under continuous antibiotic treatment. Molecular fingerprinting based on single-strand conformation polymorphism (SSCP) analysis revealed fundamental differences between bacterial and fungal communities. Both groups of microorganisms were taxonomically classified by identification of gene sequences (16S rRNA and internal transcript spacer), and prevalences of single taxa were determined for the entire cohort. Major bacterial pathogens were frequently observed, whereas fungi of known pathogenicity in CF were detected only in low numbers. Fungal species richness increased without reaching a constant level (saturation), whereas bacterial richness showed saturation after 50 patients were analyzed. In contrast to bacteria, a large number of fungal species were observed together with high fluctuations over time and among patients. These findings demonstrated that the mycobiome was dominated by transient species, which strongly suggested that the main driving force was their presence in inhaled air rather than colonization. Considering the high exposure of human airways to fungal spores, we concluded that fungi have low colonization abilities in CF, and colonization by pathogenic fungal species may be considered a rare event. A comprehensive understanding of the conditions promoting fungal colonization may offer the opportunity to prevent colonization and substantially reduce or even eliminate fungus-related disease progression in CF.

show abstract

“…In a matched case-control study, Regev-Yochay et al 15 reported that the odds of co-colonization with S. aureus were significantly lower for individuals carrying a piliated versus nonpiliated S. pneumoniae strain. Wos-Oxley et al 16 and Costello et al 17 both suggest that the anterior nares represent a complex ecological niche in which the species interaction may well determine the colonization and prevalence of S. aureus and MRSA.…”

mentioning

confidence: 99%

Prevalence of Staphylococcus aureus and Lack of Its Lytic Bacteriophages in the Anterior Nares of Patients and Healthcare Workers at a Rural Clinic

Aswani¹,

Shukla²

2010

Clinical Medicine & Research

View full text Add to dashboard Cite

Background: Nearly 30% of people in the United States are colonized with Staphylococcus aureus and 1% to 2% with methicillin-resistant Staphylococcus aureus (MRSA) in the anterior nares. However, it is not known if lytic bacteriophages against S. aureus are present in the anterior nares, and if they are, what the prevalence rate is, or if they interfere with S. aureus colonization. The aim of this study was to determine the prevalence of nasal carriage of S. aureus and MRSA and to screen for S. aureus lytic bacteriophages in healthcare environment workers and ambulatory patients.

show abstract

A poke into the diversity and associations within human anterior nare microbial communities

Cited by 105 publications

References 40 publications

Topographical Continuity of Bacterial Populations in the Healthy Human Respiratory Tract

Topographical Continuity of Bacterial Populations in the Healthy Human Respiratory Tract

Cohort Study of Airway Mycobiome in Adult Cystic Fibrosis Patients: Differences in Community Structure between Fungi and Bacteria Reveal Predominance of Transient Fungal Elements

Prevalence of Staphylococcus aureus and Lack of Its Lytic Bacteriophages in the Anterior Nares of Patients and Healthcare Workers at a Rural Clinic

Contact Info

Product

Resources

About