A Poisson-Independent Approach to Precision Nucleic Acid Quantification in Microdroplets
Reya Ganguly,
Chang-Soo Lee
Abstract:Digital PCR (dPCR) has become indispensable in nucleic acid (NA) detection across various fields, including viral diagnostics and mutant detection. However, misclassification of partitions in dPCR can significantly impact accuracy. Despite existing methods to minimize misclassification bias, accurate classification remains elusive, especially for nonamplified target partitions. To address these challenges, this study introduces an innovative microdroplet-based competitive PCR platform for nucleic acid quantifi… Show more
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