A Point-of-Care Prothrombin Time Test on a Microfluidic Disk Analyzer Using Alternate Spinning

Lin, Chien-Cheng; Lin, Kun‐Wei; Yen, Daniel; Shih, Chih-Hsin; Lu, Chien-Hsing; Wang, Jiunn-Min; Lin, Chi-Yu

doi:10.1166/jnn.2015.9533

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…Compared with other microfluidic chips, disc microfluidic chips have the ability to centrifuge and separate whole blood samples due to their structural advantages. On this basis, disc microfluidic technology can directly carry detection methods consistent with laboratory tests, which is more conducive to building a diagnostic system for consistency between clinical and laboratory coagulation function test results (Lin CH, et al, 2015).…”

Section: Dynamic Current Methodmentioning

confidence: 99%

Coagulation Function Testing Clinical Demand and Current Status in China

Li,

Chen,

Zhang

et al. 2024

JIMR

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Diseases or symptoms related to coagulation function are widely present in clinical daily work, as coagulation function plays a central role in maintaining homeostasis in the internal environment. Therefore, coagulation dysfunction often leads to serious harm to patients. At the same time, coagulation related treatments such as the use of anticoagulants or transfusion therapy have potential serious side effects, even leading to patient death. For many years, balancing the bleeding or embolism caused by anticoagulants has been a challenge that clinical workers have to face, which poses great challenges to clinical work. Therefore, monitoring coagulation function is very important for clinical diagnosis and treatment. The rapid and accurate completion of coagulation function diagnosis conclusions and early transmission to clinical workers are important guarantees for patient life. However, at present, due to technical limitations and the complexity of the detection process, this demand cannot be met. At present, in China, coagulation function testing is mostly concentrated in laboratory departments, and the limitation of distance may affect the timeliness of coagulation function testing. Therefore, many clinical departments of institutions tend to conduct coagulation function testing themselves, which has led to many new demands for coagulation function testing, such as fast and convenient POCT coagulation function testing and coagulation function testing of cell models that are more in line with clinical needs, More joint detection of coagulation markers and so on, of course, these needs must be established on the premise that clinical testing equipment and laboratory testing results are consistent. However, in order to meet the needs of miniaturization and rapid testing, the coagulation function testing equipment used in clinical departments often has significant methodological differences from laboratory testing equipment, which further leads to differences in testing results. Based on this situation, many new technologies, including microfluidic technology, as well as more detection techniques, have been widely and deeply applied to clinical coagulation function testing, which has brought a lot of good news to clinical coagulation function diagnosis to a large extent.

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Section: Dynamic Current Methodmentioning

confidence: 99%

Coagulation Function Testing Clinical Demand and Current Status in China

Li,

Chen,

Zhang

et al. 2024

JIMR

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“…This system reduced the processing time and reagent consumption by an order of magnitude. Lin et al [20] demonstrated a POC device in which decanting and mixing were performed by alternating the spin direction, and the process was completed within 2 s.…”

Section: Fabrication Of Sample Cartridgementioning

confidence: 99%

A Nut-and-Bolt Microfluidic Mixing System for the Rapid Labeling of Immune Cells with Antibodies

Imran

Kim

2020

Micromachines

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A nut-and-bolt microfluidic system was previously developed for a point-of-care (POC) human immunodeficiency virus (HIV) test and was able to acquire images of CD4 (cluster of differentiation 4) + T-lymphocytes in a sample drop of blood followed by image analysis. However, as the system was not fully integrated with a sample reaction module, the mixing of the sample with the antibody reagent was carried out manually. To achieve a rapid reaction with a reduced amount of costly reagent in a POC diagnostic system, an efficient sample mixing function must be implemented. Here, we propose a novel method to drastically accelerate the process of sample mixing and increase the reaction rate in the nut-and-bolt microfluidic system, where the sample is mixed with the reagent in a reaction chamber formed by connecting a nut with a bolt-like sample cartridge. The mixing is facilitated by rotating the sample cartridge bidirectionally using a DC motor, which agitates the sample in a chaotic manner. A microbead complex formed by the avidin–biotin interaction was used as a model reaction system to examine the feasibility of our mixing module. We found that the reaction time for the avidin–biotin binding by mixing was 7.5 times shorter than in the incubation method, achieving a reaction efficiency of over 95%. The performance of our mixing system was further demonstrated by measuring the concentration of CD4 cells labeled with a fluorescent antibody in the blood sample. The antigen–antibody reaction mixing was faster by a factor of 20, reaching a reaction efficiency comparable to the conventional incubation method.

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“…The motor control system, which is capable of conducting precise spinning control and is used to evaluate the performance of the fluidic function, was mentioned in our previous work. 7,24 To observe the fluid flow under rotation, a high-speed complementary metal oxide semiconductor camera (AVT BONITO CL-400C, Allied Vision, USA) and a stroboscope (DT-311N, SHIMPO, Japan) were used to capture images of the disk at the same location. The images were analyzed using computer software (Vision Assistant 2015, Nation Instrument, USA) to acquire information required for liquid volume quantification and flow field analysis.…”

Section: Disk Fabrication and Flow Visualizationmentioning

confidence: 99%

Disk-based enzyme-linked immunosorbent assays using the liquid-aliquoting and siphoning-evacuation technique

Chen

Shih

2018

Biomicrofluidics

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A cost-effective way to carry out multiple enzyme-linked immunosorbent assays (ELISAs) on a centrifugal platform using the liquid-aliquoting and siphoning-evacuation (LASE) technique was developed in this paper. Instead of preloading all the reagents in the reservoirs before testing, each reagent was loaded only one time during testing. The reagent was distributed into equal aliquots and delivered into reaction chambers by the aliquoting fluidic function. In addition, a siphoning-evacuation technique was developed to improve the washing efficiency and simplify the assay protocol. Furthermore, the entire assay protocol can be conducted using a two-step spinning protocol, which greatly reduces the cost of the motor control system. With the LASE technique, a low-cost and user-friendly ELISA system can be achieved.

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A Point-of-Care Prothrombin Time Test on a Microfluidic Disk Analyzer Using Alternate Spinning

Cited by 8 publications

References 32 publications

Coagulation Function Testing Clinical Demand and Current Status in China

Coagulation Function Testing Clinical Demand and Current Status in China

A Nut-and-Bolt Microfluidic Mixing System for the Rapid Labeling of Immune Cells with Antibodies

Disk-based enzyme-linked immunosorbent assays using the liquid-aliquoting and siphoning-evacuation technique

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