A platform for specific and sensitive detection of target bacteria by selective magnetic enrichment and a broad-spectrum fluorescent probe

Sun, Jiejie; Zhang, Li; Xu, Yingchun; Xue, Yuyan; Qiao, Liang; Ding, Chuan‐Fan; Ling, Ling; Yu, Shaoning

doi:10.1016/j.snb.2021.130762

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…The probe can be easily prepared by dissolving europium acetate in an aqueous solution. In contrast, sensing probes produced by other existing methods required varying durations, ranging from a few tens of minutes to several tens of hours. − As mentioned earlier, the LOD of our method for S. aureus was approximately 10 4 CFU mL –1 , determined through the calibration curve presented in Supporting Information Figure S10. When our LOD is compared with those obtained from other existing methods, it becomes apparent that our LOD is higher than those achieved by the existing methods.…”

Section: Resultsmentioning

confidence: 86%

Europium Ion-Based Magnetic-Trapping and Fluorescence-Sensing Method for Detection of Pathogenic Bacteria

Jannatin,

Yang,

et al. 2024

Anal. Chem.

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Europium ions (Eu 3+ ) have been utilized as a fluorescence-sensing probe for a variety of analytes, including tetracycline (TC). When Eu 3+ is chelated with TC, its fluorescence can be greatly enhanced. Moreover, Eu 3+ possesses 6 unpaired electrons in its f orbital, which makes it paramagnetic. Being a hard acid, Eu 3+ can chelate with hard bases, such as oxygen-containing functional groups (e.g., phosphates and carboxylates), present on the cell surface of pathogenic bacteria. Due to these properties, in this study, Eu 3+ was explored as a magnetic-trapping and sensing probe against pathogenic bacteria present in complex samples. Eu 3+ was used as a magnetic probe to trap bacteria such as Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Acinetobacter baumannii, Bacillus cereus, and Pseudomonas aeruginosa. The addition of TC facilitated the easy detection of magnetic Eu 3+ −bacterium conjugates through fluorescence spectroscopy, with a detection limit of approximately ∼10 4 CFU mL −1 . Additionally, matrix-assisted laser desorption/ionization mass spectrometry was employed to differentiate bacteria tapped by our magnetic probes.

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Section: Resultsmentioning

confidence: 86%

Europium Ion-Based Magnetic-Trapping and Fluorescence-Sensing Method for Detection of Pathogenic Bacteria

Jannatin,

Yang,

et al. 2024

Anal. Chem.

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“…The LODs of SA and MRSA are comparable with those reported in the literature (Table S1). − The result indicates that the as-proposed strategy can be used for quantitative detection of SA and MRSA with high sensitivity. In addition, the intensities of corresponding green fluorescence images are increased by increasing the concentrations of SA and MRSA, while the intensities of corresponding red fluorescence images are only increased by increasing the concentration of MRSA.…”

Section: Resultsmentioning

confidence: 90%

A Multichannel Fluorescence Isothermal Amplification Device with Integrated Internet of Medical Things for Rapid Sensing of Pathogens through Deep Learning

Sun,

Shan,

Jian

et al. 2023

Anal. Chem.

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The landscape of diagnostic assessments has experienced a paradigm shift driven by the advent of isothermal amplification techniques on point-of-care testing (POCT). The development of compact, portable isothermal amplification devices further emphasizes their transformative influence on diagnostic approaches. However, in prioritizing portability, these devices may exhibit limitations in functionality, rendering them less effective in addressing urgent public health emergencies during sudden pathogen outbreaks. In this paper, an efficient isothermal fluorescence amplification device has been fabricated for the rapid detection of pathogens during public health crises. The device features multichannel capability for simultaneous detection of various targets, integrates with the Internet of Medical Things (IoMT) for remote control and data uploading, and includes a deep learning-based batch processing system for rapid (9.4 ms) and accurate discrimination of pathogen type with excellent accuracy. The device has been successfully employed to simultaneously detect Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus aureus (MRSA) with limits of detection (LODs) of 18 CFU/mL (SA) and 20 CFU/mL (MRSA) within 35 min by multiplex RPA assay and CRISPR/Cas12a-mediated nucleic acid detection assay.

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“…Fluorescent nanoprobes coupled with magnetic nanoparticles have demonstrated great application prospects in separation and detection, enabling rapid recognition and sensitive detection of trace proteins in complex real samples. [133][134][135] Liposome-like C dots and C dot-nanovesicles with brightness and insignificant blinking are efficient drug/dye carriers, showing their potential for sensing, cell imaging, and cell tracking. [132] It is thus highly expected that multifunctional C dots and MNCs can be prepared by carefully selecting ligands/precursors.…”

Section: Discussionmentioning

confidence: 99%

Fluorescent carbon dots and noble metal nanoclusters for sensing applications: Minireview

Lin

Yang

Huang

2022

J Chinese Chemical Soc

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Nanomaterial‐based fluorescence sensing systems with the advantages of sensitivity and selectivity have been popular for quantitation of various analytes, including biomolecules, metal ions, and organic molecules. Fluorescent carbon dots (C dots) and noble metal nanoclusters (MNCs) separately composed of few carbon and metal atoms have attracted considerable research interest in the past decade, mainly because of their biocompatibility, as well as ease in preparation and conjugation. This review briefly discusses simple and popular strategies for the preparation of C dots and MNCs. Their sensing applications in monitoring pH values, quantitation of metal ions, and small molecules are emphasized. Their future use in conjunction with portable microsystems such as array and microfluidic device to develop high‐throughput and sensitive sensing systems for on‐site analysis is also discussed.

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A platform for specific and sensitive detection of target bacteria by selective magnetic enrichment and a broad-spectrum fluorescent probe

Cited by 7 publications

References 31 publications

Europium Ion-Based Magnetic-Trapping and Fluorescence-Sensing Method for Detection of Pathogenic Bacteria

Europium Ion-Based Magnetic-Trapping and Fluorescence-Sensing Method for Detection of Pathogenic Bacteria

A Multichannel Fluorescence Isothermal Amplification Device with Integrated Internet of Medical Things for Rapid Sensing of Pathogens through Deep Learning

Fluorescent carbon dots and noble metal nanoclusters for sensing applications: Minireview

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