A Plastid RNA Polymerase-Associated Protein Is Involved in Early Chloroplast Development in Rice

Song, Shuang; Wang, Ying; Ding, Xin; Tian, Yunlu; Wu, Zewan; Li, Hang; Li, Qing; Wang, Yunpeng; Zhou, Shirong; Dong, Xiaoou; Wan, Jianmin; Liu, Linglong

doi:10.3390/agronomy13051424

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…HT modifies the permeability and affects the fluidity of membranes in plants. It also impairs the photosynthetic activity by inactivating and degrading enzymes responsible promoting ROS accumulation (Liu et al 2023a, Liu et al 2023b, Song et al 2023, Yang et al 2023.…”

Section: Mutation Of Pgl10 Promotes Ros Accumulationmentioning

confidence: 99%

Loss‐of‐function of PGL10 impairs photosynthesis and tolerance to high‐temperature stress in rice

Ahmad,

Tabassum,

Sheng

et al. 2024

Physiologia Plantarum

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High temperature (HT) affects the production of chlorophyll (Chl) pigment and inhibits cellular processes that impair photosynthesis, and growth and development in plants. However, the molecular mechanisms underlying heat stress in rice are not fully understood yet. In this study, we identified two mutants varying in leaf color from the ethylmethanesulfonate mutant library of indica rice cv. Zhongjiazao‐17, which showed pale‐green leaf color and variegated leaf phenotype under HT conditions. Mut‐map revealed that both mutants were allelic, and their phenotype was controlled by a single recessive gene PALE GREEN LEAF 10 (PGL10) that encodes NADPH:protochlorophyllide oxidoreductase B, which is required for the reduction of protochlorophyllide into chlorophyllide in light‐dependent tetrapyrrole biosynthetic pathway‐based Chl synthesis. Overexpression‐based complementation and CRISPR/Cas9‐based knockout analyses confirmed the results of Mut‐map. Moreover, qRT‐PCR‐based expression analysis of PGL10 showed that it expresses in almost all plant parts with the lowest expression in root, followed by seed, third leaf, and then other green tissues in both mutants, pgl10a and pgl10b. Its protein localizes in chloroplasts, and the first 17 amino acids from N‐terminus are responsible for signals in chloroplasts. Moreover, transcriptome analysis performed under HT conditions revealed that the genes involved in the Chl biosynthesis and degradation, photosynthesis, and reactive oxygen species detoxification were differentially expressed in mutants compared to WT. Thus, these results indicate that PGL10 is required for maintaining chloroplast function and plays an important role in rice adaptation to HT stress conditions by controlling photosynthetic activity.

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Section: Mutation Of Pgl10 Promotes Ros Accumulationmentioning

confidence: 99%

Loss‐of‐function of PGL10 impairs photosynthesis and tolerance to high‐temperature stress in rice

Ahmad,

Tabassum,

Sheng

et al. 2024

Physiologia Plantarum

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“…The accumulation of ROS can also damage cells and negatively affect organisms, and oxidative stress signaling can be induced by ROS molecules in cells to protect or mitigate damage [75]. ROS comprise many species, such as singlet oxygen ( 1 O 2 ), superoxide anions (O 2 − ), hydroxyl radicals (OH) and hydrogen peroxide (H 2 O 2 ), which affect proteins, lipids, and DNA repairment, and they simultaneously impact gene expression as signals [76]. The pattern of nitro blue tetrazolium (NBT) staining reflected the formation of blue formazan precipitates and was indicative of O 2 − accumulation in the leaves of the ygl19 mutant compared to the wild type (Figure 12A).…”

Section: The Mutation Of the Ygl19 Gene Affects The Expression Of Pho...mentioning

confidence: 99%

Yellow-Green Leaf 19 Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice

Wang,

Zhang,

Wei

et al. 2023

IJMS

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Chlorophyll is the main photosynthetic pigment and is crucial for plant photosynthesis. Leaf color mutants are widely used to identify genes involved in the synthesis or metabolism of chlorophyll. In this study, a spontaneous mutant, yellow-green leaf 19 (ygl19), was isolated from rice (Oryza sativa). This ygl19 mutant showed yellow-green leaves and decreased chlorophyll level and net photosynthetic rate. Brown necrotic spots appeared on the surface of ygl19 leaves at the tillering stage. And the agronomic traits of the ygl19 mutant, including the plant height, tiller number per plant, and total number of grains per plant, were significantly reduced. Map-based cloning revealed that the candidate YGL19 gene was LOC_Os03g21370. Complementation of the ygl19 mutant with the wild-type CDS of LOC_Os03g21370 led to the restoration of the mutant to the normal phenotype. Evolutionary analysis revealed that YGL19 protein and its homologues were unique for photoautotrophs, containing a conserved Ycf54 functional domain. A conserved amino acid substitution from proline to serine on the Ycf54 domain led to the ygl19 mutation. Sequence analysis of the YGL19 gene in 4726 rice accessions found that the YGL19 gene was conserved in natural rice variants with no resulting amino acid variation. The YGL19 gene was mainly expressed in green tissues, especially in leaf organs. And the YGL19 protein was localized in the chloroplast for function. Gene expression analysis via qRT-PCR showed that the expression levels of tetrapyrrole synthesis-related genes and photosynthesis-related genes were regulated in the ygl19 mutant. Reactive oxygen species (ROS) such as superoxide anions and hydrogen peroxide accumulated in spotted leaves of the ygl19 mutant at the tillering stage, accompanied by the regulation of ROS scavenging enzyme-encoding genes and ROS-responsive defense signaling genes. This study demonstrates that a novel yellow-green leaf gene YGL19 affects tetrapyrrole biosynthesis, photosynthesis, and ROS metabolism in rice.

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Comparative Transcriptome Combined with Morphophysiological Analyses Revealed Carotenoid Biosynthesis for Differential Chilling Tolerance in Two Contrasting Rice (Oryza sativa L.) Genotypes

Zhang,

Wu,

Chen

et al. 2023

Rice

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Early spring cold spells can lead to leaf chlorosis during the rice seedling greening process. However, the physiological and molecular mechanisms underlying the rice greening process under low-temperature conditions remain unknown. In this study, comparative transcriptome and morphophysiological analyses were performed to investigate the mechanisms mediating the responses of the Koshihikari (Kos) and Kasalath (Kas) rice cultivars to chilling stress. According to their growth-related traits, electrolyte leakage, and chlorophyll fluorescence parameters, Kos was more tolerant to low-temperature stress than Kas. Moreover, chloroplast morphology was more normal (e.g., oval) in Kos than in Kas at 17 °C. The comparative transcriptome analysis revealed 610 up-regulated differentially expressed genes that were common to all four comparisons. Furthermore, carotenoid biosynthesis was identified as a critical pathway for the Kos response to chilling stress. The genes in the carotenoid biosynthesis pathway were expressed at higher levels in Kos than in Kas at 17 °C, which was in accordance with the higher leaf carotenoid content in Kos than in Kas. The lycopene β-cyclase and lycopene ε-cyclase activities increased more in Kos than in Kas. Additionally, the increases in the violaxanthin de-epoxidase and carotenoid hydroxylase activities in Kos seedlings resulted in the accumulation of zeaxanthin and lutein and mitigated the effects of chilling stress on chloroplasts. These findings have clarified the molecular mechanisms underlying the chilling tolerance of rice seedlings during the greening process.

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A Plastid RNA Polymerase-Associated Protein Is Involved in Early Chloroplast Development in Rice

Cited by 5 publications

References 55 publications

Loss‐of‐function of PGL10 impairs photosynthesis and tolerance to high‐temperature stress in rice

Loss‐of‐function of PGL10 impairs photosynthesis and tolerance to high‐temperature stress in rice

Yellow-Green Leaf 19 Encoding a Specific and Conservative Protein for Photosynthetic Organisms Affects Tetrapyrrole Biosynthesis, Photosynthesis, and Reactive Oxygen Species Metabolism in Rice

Comparative Transcriptome Combined with Morphophysiological Analyses Revealed Carotenoid Biosynthesis for Differential Chilling Tolerance in Two Contrasting Rice (Oryza sativa L.) Genotypes

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