A Plasmodium falciparum GLURP–MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodies

Theisen, Michael; Soé, Soe; Brunstedt, Katja; Follmann, Frank; Bredmose, Lars; Israelsen, Hans; Madsen, Søren; Druilhe, Pierre

doi:10.1016/j.vaccine.2003.09.017

Cited by 84 publications

(55 citation statements)

References 36 publications

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“…Antibody titres were then defined as the serum dilution that resulted in an absorbance of 1Á00 in the parallel portion of the curves as previously described. 21 As the gene for IgG2a is deleted in C57BL/6 mice, 22 titres of IgG2b were evaluated in this strain.…”

Section: Evaluation Of Antibody Titresmentioning

confidence: 99%

Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection

et al. 2008

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SummaryIt is known that protection against tuberculosis is mediated primarily by T helper type 1 (Th1) cells but the influence of the Th1/Th2 balance of a vaccination response on the subsequent protection and pathology during infection has not been studied in detail. We designed a panel of Ag85B-ESAT-6 subunit vaccines based on adjuvants with different Th1/Th2-promoting activities and studied cellular responses, bacterial replication and pathology in the lungs of mice infected with Mycobacterium tuberculosis. All vaccines induced cell-mediated and humoral responses but with markedly different interferon-c : interleukin-5 (IFN-c : IL-5) and immunoglobulin G1 (IgG1) : IgG2 ratios. The vaccines promoted different levels of control of bacterial replication with the most efficient protection being exerted by cationic liposomes containing monophosphoryl lipid A and low to completely absent immunity with conventional aluminium. The level of protection correlated with the amount of IFN-c produced in response to the vaccine whereas there was no inverse correlation with the level of IL-5. Characterizing a protective response was an accelerated recruitment of IL-17 and IFN-c-producing lymphocytes resulting in the early formation of granulomas containing clustered inducible nitric oxide synthase-activated macrophages. In comparison, non-protected mice exhibited a different inflammatory infiltrate rich in neutrophil granulocytes. This study indicates that the adjuvant component of a tuberculosis vaccine may be crucial in determining the kinetics by which effective granulomas, pivotal in controlling bacterial growth, are formed.

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Section: Evaluation Of Antibody Titresmentioning

confidence: 99%

Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection

et al. 2008

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“…It is generally believed that a combination vaccine for malaria is likely to be more effective than vaccines based on a single antigen, and attempts are being made to develop a malaria vaccine by using a mixture of more than one antigen or by combining immunologically relevant proteins of the target antigens as fusion proteins (31,43,45). In the present study, we have constructed a fusion chimera (MSP-Fu 24 ) consisting of PfMSP-1 19 and PfMSP-3 11 and produced the corresponding recombinant MSP-Fu 24 protein in Escherichia coli cells.…”

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confidence: 99%

Plasmodium falciparumMerozoite Surface Protein 1 (MSP-1)-MSP-3 Chimeric Protein: Immunogenicity Determined with Human-Compatible Adjuvants and Induction of Protective Immune Response

et al. 2010

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“…The data were fitted by nonlinear regression analysis with a sigmoidal dose-response curve of variable slope by the GraphPad Prism program (version 4.00; GraphPad Software Inc.). Antibody titers were then defined as the serum dilution that gives an absorbance value of 1.00 in the parallel portion of the curves (39). For serum samples where the antibody titration curves were below this value, the titer was defined as below the dilution 20 (Ͻ20).…”

mentioning

confidence: 99%

Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System

et al. 2005

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The immunostimulation provided by the mycobacterial cell wall has been exploited for many decades, e.g., in Freund's complete adjuvant. Recently, the underlying mechanism behind this adjuvant activity, including Toll receptor signaling, has begun to be unraveled, confirming the potential of mycobacterial constituents to act as adjuvants. In this study, the immunostimulatory properties of a Mycobacterium bovis BCG lipid extract were tested for their adjuvant activity. Administration of the lipids in dimethyl dioctadecyl ammonium bromide-based cationic liposomes induced a powerful Th1 response characterized by markedly elevated antigen-specific immunoglobulin G2a (IgG2a) isotype antibodies and substantial production of gamma interferon. The adjuvant formulation (designated mycosomes) elicited high levels of gamma interferon both in C57BL/6 as well as in Th2-prone BALB/c mice. Furthermore, the mycosomes induced immune responses to protein antigens from several sources including Mycobacterium tuberculosis, Chlamydia muridarum, and tetanus toxoid. In a tuberculosis challenge model, the mycosomes combined with the Ag85B-ESAT-6 fusion protein were demonstrated to have a unique ability to maintain sustained immunological memory at a level superior to live BCG.

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A Plasmodium falciparum GLURP–MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodies

Cited by 84 publications

References 36 publications

Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection

Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection

Plasmodium falciparumMerozoite Surface Protein 1 (MSP-1)-MSP-3 Chimeric Protein: Immunogenicity Determined with Human-Compatible Adjuvants and Induction of Protective Immune Response

Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System

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