A plasmid-encoded UmuD homologue regulates expression of Pseudomonas aeruginosa SOS genes

Díaz-Magaña, Amada; Alva-Murillo, Nayeli; Chávez-Moctezuma, Martha P.; López‐Meza, Joel E.; Ramírez-Díaz, Martha I.; Cervantes, Carlos

doi:10.1099/mic.0.000103

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…The pUM505 plasmid is a conjugative plasmid isolated from a clinical isolate of P. aeruginosa (9). pUM505 carries different genes, which encode proteins involved in metal resistance, DNA repair, virulence, and plasmid stability (10)(11)(12)(13). Additionally, pUM505 conferred CIP resistance on P. aeruginosa PU21 (13), suggesting that pUM505 contains genes responsible for quinolone resistance.…”

Section: Discussionmentioning

confidence: 99%

“…pUM505 is a self-conjugating 123-kbp plasmid isolated from a clinical Pseudomonas aeruginosa isolate (9). This plasmid carries several adaptive genes, including the umuD gene (encoding a transcriptional regulator of the SOS response) (10), genes involved in Pseudomonas virulence (11,12), and genes that increase plasmid stability (12). The aim of this work was to identify and study the product of the orf131 (renamed crpP) gene on pUM505, which confers resistance to CIP.…”

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confidence: 99%

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CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Chávez-Jacobo

Hernández-Ramírez

Romo-Rodríguez

et al. 2018

Antimicrob Agents Chemother

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The pUM505 plasmid, isolated from a clinical isolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard strain PAO1. CIP is an antibiotic of the quinolone family that is used to treat infections. analysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the gene in pUM505 displays 40% amino acid identity to the aminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned (renamed, for iprofloxacinesistance rotein,lasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC-, conferred resistance to CIP on strain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography-tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in, which involves phosphorylation of the antibiotic.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Chávez-Jacobo

Hernández-Ramírez

Romo-Rodríguez

et al. 2018

Antimicrob Agents Chemother

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“…The induction of PrtR-managed genes adversely affects survival during genotoxic stress, decreases antimicrobial resistance, and reduces resistance to oxidant agents (204). In addition, the UmuDpR protein has been demonstrated to suppress expression of P. aeruginosa SOS genes regulated by LexA (205).…”

Section: Sos Responsementioning

confidence: 99%

Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments

et al. 2018

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SUMMARYPathogens that infect the gastrointestinal and respiratory tracts are subjected to intense pressure due to the environmental conditions of the surroundings. This pressure has led to the development of mechanisms of bacterial tolerance or persistence which enable microorganisms to survive in these locations. In this review, we analyze the general stress response (RpoS mediated), reactive oxygen species (ROS) tolerance, energy metabolism, drug efflux pumps, SOS response, quorum sensing (QS) bacterial communication, (p)ppGpp signaling, and toxin-antitoxin (TA) systems of pathogens, such as , spp., spp., spp., , spp., spp., spp., and , all of which inhabit the gastrointestinal tract. The following respiratory tract pathogens are also considered:, ,, , and Knowledge of the molecular mechanisms regulating the bacterial tolerance and persistence phenotypes is essential in the fight against multiresistant pathogens, as it will enable the identification of new targets for developing innovative anti-infective treatments.

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“…Intriguingly, a plasmid-encoded umuD gene has previously been shown to regulate the SOS response in Pseudomonas aeruginosa (Díaz-Magaña et al, 2015), suggesting that plasmids may directly manipulate expression of the bacterial SOS response. It is hypothesised the production of conjugation apparatus may lead to misfolded proteins; F047 failed to conjugate in shaking conditions.…”

Section: Discussionmentioning

confidence: 99%

Limited and strain-specific transcriptional and growth responses to acquisition of a multidrug resistance plasmid in genetically diverseEscherichia colilineages

Dunn

Carrilero

Brockhurst

et al. 2020

Preprint

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Multi-drug resistant (MDR) Escherichia coli are a major global threat to human health, wherein multi-drug resistance is primarily spread by MDR plasmid acquisition. MDR plasmids are not widely distributed across the entire E. coli species, but instead are concentrated in a small number of clones. Here, we test if diverse E. coli strains vary in their ability to acquire and maintain MDR plasmids, and if this relates to their transcriptional response following plasmid acquisition. We used strains from across the diversity of E. coli, including the common MDR lineage ST131, and the IncF plasmid, pLL35, encoding multiple antibiotic resistance genes. Strains varied in their ability to acquire pLL35 by conjugation, but all were able to stably maintain the plasmid. The effects of pLL35 acquisition on cefotaxime resistance and growth also varied among strains, with growth responses ranging from a small decrease to a small increase in growth of the plasmid-carrier relative to the parental strain. Transcriptional responses to pLL35 acquisition were limited in scale and highly strain specific. We observed significant transcriptional responses at the operon or regulon level, possibly due to stress responses or interactions with resident MGEs. Subtle transcriptional responses consistent across all strains were observed affecting functions, such as anaerobic metabolism, previously shown to be under negative frequency dependent selection in MDR E. coli. Overall there was no correlation between the magnitude of the transcriptional and growth responses across strains. Together these data suggest that fitness costs arising from transcriptional disruption are unlikely to act as a barrier to MDR plasmid dissemination in E. coli.

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A plasmid-encoded UmuD homologue regulates expression of Pseudomonas aeruginosa SOS genes

Cited by 10 publications

References 34 publications

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments

Limited and strain-specific transcriptional and growth responses to acquisition of a multidrug resistance plasmid in genetically diverseEscherichia colilineages

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