A plasmid-encoded mobile genetic element from Pseudomonas aeruginosa that confers heavy metal resistance and virulence

Hernández-Ramírez, Karen C.; Reyes-Gallegos, Rosa I.; Chávez-Jacobo, Víctor M.; Díaz-Magaña, Amada; Meza-Carmen, Víctor; Ramírez-Díaz, Martha I.

doi:10.1016/j.plasmid.2018.07.003

Cited by 16 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is especially important as Pseudomonas plasmids frequently carry phenotypic genetic modules of adaptive value. Many of these replicons are associated with the utilization of toxic compounds and resistance to toxic metals or antibiotics, e.g., pWW0 [13] and NAH7 [14], which encode proteins involved in the degradation of toluene and naphthalene, or pUM505—conferring chromate and mercury resistance [15].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Romaniuk

Styczyński

Decewicz

et al. 2019

Genes

View full text Add to dashboard Cite

Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of cold-active Pseudomonas spp. originating from the King George Island (Antarctica) were characterized using a combined, structural and functional approach, including thorough genomic analyses, functional analyses of selected genetic modules, and identification of active transposable elements localized within the plasmids and comparative genomics. The analyses performed in this study increased the understanding of the horizontal transfer of plasmids found within Pseudomonas populations inhabiting Antarctic soils. It was shown that the majority of the studied plasmids are narrow-host-range replicons, whose transfer across taxonomic boundaries may be limited. Moreover, structural and functional analyses enabled identification and characterization of various accessory genetic modules, including genes encoding major pilin protein (PilA), that enhance biofilm formation, as well as active transposable elements. Furthermore, comparative genomic analyses revealed that the studied plasmids of Antarctic Pseudomonas spp. are unique, as they are highly dissimilar to the other known plasmids of Pseudomonas spp.

show abstract

Section: Introductionmentioning

confidence: 99%

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Romaniuk

Styczyński

Decewicz

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…Another interesting observation was the association of the umuC gene with a gene encoding a putative mercury resistance protein in two strains (HIAE_PA05 and HIAE_PA16). In previous studies it was found that mercury resistance genes are located on mobile genetic elements such as plasmids and transposons in Pseudomonas aeruginosa and other Pseudomonas species (Hernández-Ramírez et al, 2018; Kottara et al, 2018). To know more about the occurrence of mercury resistance, we checked the 214 complete P. aeruginosa genomes: a gene for putative mercury resistance is found in 82 strains (38% of all strains).…”

Section: Resultsmentioning

confidence: 99%

Mutagenesis Induced by Sub-Lethal Doses of Ciprofloxacin: Genotypic and Phenotypic Differences Between the Pseudomonas aeruginosa Strain PA14 and Clinical Isolates

et al. 2019

View full text Add to dashboard Cite

Bacterial resistance is a severe threat to global public health. Exposure to sub-lethal concentrations has been considered a major driver of mutagenesis leading to antibiotic resistance in clinical settings. Ciprofloxacin is broadly used to treat infections caused by Pseudomonas aeruginosa , whereas increased mutagenesis induced by sub-lethal concentrations of ciprofloxacin has been reported for the reference strain, PAO1, in vitro . In this study we report increased mutagenesis induced by sub-lethal concentrations of ciprofloxacin for another reference strain, PA14-UCBPP, and lower mutagenesis for clinical isolates when compared to the reference strain. This unexpected result may be associated with missense mutations in imuB and recX , involved in adaptive responses, and the presence of Pyocin S2, which were found in all clinical isolates but not in the reference strain genome. The genetic differences between clinical isolates of P. aeruginosa and the reference PA14-UCBPP, often used to study P. aeruginosa phenotypes in vitro , may be involved in reduced mutagenesis under sub-lethal concentrations of CIP, a scenario that should be further explored for the understanding of bacterial fitness in hospital environments. Moreover, we highlight the presence of a complete umuDC operon in a P. aeruginosa clinical isolate. Even though the presence of umuDC did not contribute to a significant increase in mutagenesis, it highlights the dynamic exchange of genetic material between bacterial species in the hospital environment.

show abstract

“…Finally, conjugative plasmid (pUM505) isolated from Pseudomonas aeruginosa possesses a putative (31.292 kb) mobile element in addition to possessing chr genes that confer chromate resistance to Pseudomonas contains two putative mer operons which could confer resistance of mercury. Furthermore, the Mpe contains genes related with the virulence of Pseudomonas aeruginosa [60].…”

Section: Evaluation Of Resistant Genes In Pseudomonas Aeruginosamentioning

confidence: 99%

Trends in Heavy Metals Tolerance and Uptake byPseudomonas aeruginosa

Abdelbary¹,

El-Gamal²,

Farrag³

2019

Pseudomonas Aeruginosa - An Armory Within

View full text Add to dashboard Cite

Pseudomonas aeruginosa is considered as the most potent bacterial strain for solving heavy metals pollution problems. Pollution is the most of problems in our world which causing a lot of risks to human, animal, plant and ecosystem. Heavy metals pollution is an ever-increasing problem in developing nations. Release of heavy metals into the environment has increased in the recent years at an alarming rate. To remove heavy metals from environment, there are different methods such as physical, chemical and biological. The biological method includes microorganisms and plant which recorded high heavy metals removal, safe and low-cost method. Microorganisms remove heavy metals from environment by different mechanisms according to their types. Thus, microbes are used as potential candidates of bioremediation that can adapt quickly to the changing noxious environment and be utilized for toxic metal remediation. In bacterial uptake and tolerance to heavy metals, Pseudomonas aeruginosa recorded potential role in bioremediation of different heavy metals with high removal percentage comparison with other bacterial strains. Chapter discusses the roles and trends of Pseudomonas aeruginosa in heavy metals tolerance and uptake as potential bacterial strain.

show abstract

A plasmid-encoded mobile genetic element from Pseudomonas aeruginosa that confers heavy metal resistance and virulence

Cited by 16 publications

References 23 publications

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Mutagenesis Induced by Sub-Lethal Doses of Ciprofloxacin: Genotypic and Phenotypic Differences Between the Pseudomonas aeruginosa Strain PA14 and Clinical Isolates

Trends in Heavy Metals Tolerance and Uptake byPseudomonas aeruginosa

Contact Info

Product

Resources

About