A Plaque Assay for Myxoma Virus Infectivity.

Schwerdt, P.R.; Schwerdt, Carlton E.

doi:10.3181/00379727-109-27318

Cited by 4 publications

(1 citation statement)

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“…Separation of adults from young was on the basis of (i) a closed versus part-closed or open fusion line (notch) between diaphysis and proximal epiphysis of the tibia (20) and (ii) an absence versus presence of a slight or prominent cartilage (lump) at the fusion line between diaphysis and distal epiphysis of the ulna (21). The tibial notch and ulnar lump were detected on live rabbits up to about 8 months old when felt through the skin, and these young were further separated into juveniles based on whether live body weight at first capture was below or above 950 g which corresponds approximately with 4 (24) and serum neutralization test (25).…”

Section: Field Methodsmentioning

confidence: 99%

Myxomatosis in farmland rabbit populations in England and Wales

Ross¹,

Tittensor²,

Fox³

et al. 1989

Epidemiol. Infect.

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SUMMARYThe overall pattern and consequences of myxomatosis in wild rabbit populations were studied at three farmland sites in lowland southern England and upland central Wales between 1971 and1978. When results from all years were combined, the disease showed a clear two-peaked annual cycle, with a main autumn peak between August and January, and a subsidiary spring peak during February to April.Rabbit fleas, the main vectors of myxomatosis in Britain, were present on fullgrown rabbits in sufficient numbers for transmission to occur throughout the year, but the observed seasonal pattern of the disease appeared to be influenced by seasonal mass movements of these fleas. However other factors were also important including the timing and success of the main rabbit breeding season, the proportion of rabbits which had recovered from the disease and the timing and extent of autumn rabbit mortality from other causes.Significantly more males than females, and more adults and immatures than juveniles, were observed to be infected by myxomatosis. Only 25-27 % of the total populations were seen to be infected during outbreaks. Using two independent methods of calculation, it was estimated that between 47 and 69 % of infected rabbits died from the disease (much lower than the expected 90-95 % for fully susceptible rabbits with the partly attenuated virus strains that predominated). Thus it was estimated that 12-19 % of the total rabbit populations were known to have died directly or indirectly from myxomatosis.Although the effects of myxomatosis were much less than during the 1950s and 1960s, it continued to be an important mortality factor. It may still have a regulatory effect on rabbit numbers, with autumn/winter peaks of disease reducing the numbers of rabbits present at the start of the breeding season.

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Section: Field Methodsmentioning

confidence: 99%

Myxomatosis in farmland rabbit populations in England and Wales

Ross¹,

Tittensor²,

Fox³

et al. 1989

Epidemiol. Infect.

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Papilloma-Myxoma Viruses

Itô

1975

Cancer a Comprehensive Treatise 2

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Nucleic acid synthesis during the focus formation by Shope fibroma virus on green monkey kidney cells

Takehara

1970

Archiv f Virusforschung

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Nucleic acid synthesis during the focus formation on Shope fibroma virus (SFV)-infected cells was studied. When African green monkey kidney (AGMK) cells were infected with SFV, the "cell-focus" was observed as a local piling up of cells at 3 to 5 days after infection. The number of loci increased in proportion to the size of the SFV inoculum. In the SFV-AGMK cell system, however, the growth rate of virus was comparatively low and no apparent cytopathic effect was demonstrated as a result of virus infection. The incorporation of aH-thymidine into the nuclear fraction of infected cells was suppressed early in the cycle of virus rep]ication and before focus formation. On the other hand, the rate of I)NA synthesis increased markedly in the infected cytoplasm. Cytoplasmic RNA synthesis also tended to increase gradually accompanying the induction of the viral DNA synthesis. In addition, the sedimentation properties of the newly synthesized DNA were investigated by sucrose density gradient centrifugation.Arch. ges. Virusforsch. 31/3--~

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A Plaque Assay for Myxoma Virus Infectivity.

Cited by 4 publications

References 0 publications

Myxomatosis in farmland rabbit populations in England and Wales

Myxomatosis in farmland rabbit populations in England and Wales

Papilloma-Myxoma Viruses

Nucleic acid synthesis during the focus formation by Shope fibroma virus on green monkey kidney cells

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