A Plant Oxysterol as a regulator of glucose homeostasis

Pandurangan, Muthuraman; Srikumar, Kotteazeth

doi:10.5138/ijpm.2010.0975.0185.02022

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…HBl subchronic exposure in rats was shown to have a strong influence on glucose homeostasis [153][154][155][156]. Experimental animals had a significant rise in the serum insulin level and decrease in the blood sugar.…”

Section: Other Effectsmentioning

confidence: 97%

Steroid plant hormones: Effects outside plant kingdom

2015

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Section: Other Effectsmentioning

confidence: 97%

Steroid plant hormones: Effects outside plant kingdom

2015

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“…When the antioxidant enzymes activity is reduced, superoxide anion and H 2 O 2 are extremely available in biological systems, stimulating the ROS production and the propagation of lipid peroxidation (Muthuraman and Srikumar, 2010). Thiobarbituric acid reactive substances (TBARS) and lipid peroxide (LOOH) are the markers of lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of betanin against streptozotocin - nicotinamide induced liver, kidney and pancreas damage by attenuating lipid byproducts and improving renal biomarkers in Wistar rats

2017

Int. J. Adv. Res. Biol. Sci

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Objective: We attempted to determine whether betanin (from natural pigments) that has antioxidant properties would be protective against diabetes induced by streptozotocin-nicotinamide in adult Wistar rats. Methods: Diabetes was induced by a single intraperitoneal (i.p.) injection of streptozotocin (STZ; 45 mg/kg b.w.) dissolved in 0.1 M citrate buffer (pH 4.5) 15 min after the i.p. administration of nicotinamide (NA; 110 mg/kg b.w.). Experimental rats were administered betanin at the dose of 20 mg/kg b.w. and glibenclamide (600 µg/kg b.w.) once a day for 30 days. Results: Untreated diabetic rats showed significantly increased levels of plasma glucose, thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH), urea, uric acid, creatinine and decreased levels of plasma insulin. Co-administration with betanin to the diabetic rats showed improved levels of plasma glucose, insulin, lipid peroxidation byproducts (LPO) and kidney biomarkers enzymes when compared to untreated diabetic rats. Moreover, histological study of kidney revealed the effect of betanin in diabetic rats. Similar kind of experimental results were obtained in glibenclamide treated diabetic rats. Conclusion: According to our current study, betanin had a beneficial role against the diabetes induced by STZ-NA in rats.

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A plant oxysterol, 28-homobrassinolide binds HMGCoA reductase catalytic cleft: stereoselective avidity affects enzyme function

et al. 2016

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Understanding the influence of ubiquitously present plant steroids on mammalian cell biology is currently of interest. Feedback inhibition of HMGCoA reductase (HMGCR) catalytic activity in the transformation of HMG-CoA to mevalonate is a significant regulatory step in sterol biosynthetic pathway. To assess the role of dietary steroids in this biochemical transformation, the phytosteroid isoform 28-homobrassinolide (28-HB), 90 % pure, obtained from Godrej Agrovet (India) was used to determine its effect on mammalian HMG-CoA reductase. Photometric assay of pure human and select rat tissue HMGCR post 28-HB oral feed, PCR-HMGCR gene expression, and in silico docking of 28-HB and HMGCoA on HMGCR protein template were carried out. Using an oral feed regimen of pure 28-HB, we noted a decrease of 16 % in liver, 17.1 % in kidney and 9.3 % in testicular HMGCR enzyme activity, 25 % in HMGCR gene expression and 44 % in the activity of pure human HMGCR due to this plant oxysterol. In silico docking studies yielded binding metrics for 28-HB-HMGCR lower than for HMGCoA-HMGCR, indicating stronger binding of HMGCR by this ligand. 28-HB exerts differential effects on rat tissue HMGCR, down regulates liver HMGCR gene expression and significantly inhibits HMGCR activity.

show abstract

A Plant Oxysterol as a regulator of glucose homeostasis

Cited by 6 publications

References 30 publications

Steroid plant hormones: Effects outside plant kingdom

Steroid plant hormones: Effects outside plant kingdom

Protective effect of betanin against streptozotocin - nicotinamide induced liver, kidney and pancreas damage by attenuating lipid byproducts and improving renal biomarkers in Wistar rats

A plant oxysterol, 28-homobrassinolide binds HMGCoA reductase catalytic cleft: stereoselective avidity affects enzyme function

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