A Plant Growth-Promoting Microbial Soil Amendment Dynamically Alters the Strawberry Root Bacterial Microbiome

Abstract

Despite growing interest in utilizing microbial-based methods for improving crop growth, much work still remains in elucidating how beneficial plant-microbe associations are established, and what role soil amendments play in shaping these interactions. Here, we describe a set of experiments that test the effect of a commercially available soil amendment, VESTA, on the soil and strawberry (Fragaria x ananassa Monterey) root bacterial microbiome. The bacterial communities of the soil, rhizosphere, and root from amendment-treated and untreated fields were profiled at four time points across the strawberry growing season using 16S rRNA gene amplicon sequencing on the Illumina MiSeq platform. In all sample types, bacterial community composition and relative abundance were significantly altered with amendment application. Importantly, time point effects on composition are more pronounced in the root and rhizosphere, suggesting an interaction between plant development and treatment effect. Surprisingly, there was slight overlap between the taxa within the amendment and those enriched in plant and soil following treatment, suggesting that VESTA may act to rewire existing networks of organisms through an, as of yet, uncharacterized mechanism. These findings demonstrate that a commercial microbial soil amendment can impact the bacterial community structure of both roots and the surrounding environment.

Microbiome dysbiosis regulates the level of energy production under anaerobic condition

“…Indicator species (IS) and network analyses were used to identify at higher taxonomic resolution of the individual bacterial and/or archaeal species with enrichment or depletion patterns in different phases of anaerobic digestion. These analyses revealed the association of different methanogenic species with increased level of CH 4 production [61], and all were observed to be more abundant in highest CH 4 producing metagenome (Group-IV). However, common indicator bacteria E. coli , Salmonella and Staphylococcus species were not found in indicator species analysis, and these findings are supported by several previous reports of the absence of common indicator bacteria after 30 days digestion in the experimental AD at different temperatures (25 °C – 45 °C) [62].…”

Irreversible sorption of carbofuran by moderately acidic soil amended with biochar

“…The combination of five biochar treatments was carried out in 10 replicates. Sample of 3 g was taken from each replicate to make carbofuran concentrations in the range of 2 to 20 mg/L (2,4,6,8,10,12,14,16,18,20 ). All these samples were thoroughly mixed and shaken for 24 hrs in an orbital shaker.…”

“…The combination of five biochar treatments with carbofuran pesticide together was carried out in 10 replicates. The carbofuran concentrations were made in the range of 1 to 20 mg/L (2,4,6,8,10,12,14,16,18,20 ).All these samples were thoroughly mixed and shaken for 24 hrs in an orbital shaker till the equilibrium was achieved. An aliquot of 5 g of soil from each treatment was taken to determine the carbofuran concentration using UV/VIS spectrophotometer at 273 nm.…”

“…The growth in agricultural productivity over the past decade has been closely linked to the use of agrochemicals such as fertilizers and pesticides. Excessive fertilizer and chemical levels added to plants have often led to soil and surface water pollution [10,11].…”

Exploring of promising bacteria from the rhizosphere of maize, cocoa and lamtoro

“…The use of PGPR is a possible alternative to reduce chemical inputs in agriculture (Etesami and Maheshwari 2018). Hence, exploration on the use of microorganisms to enhance soil fertility and plant nutrition continues to be interesting research topics reported by (Yan et al 2015;Pacheco-leyva et al 2016;Ahmad and Husain 2017;Patel et al 2017;Arfarita et al 2019;Deng et al 2019;Sheirdil et al 2019).…”