A plant-based multicomponent vaccine protects mice from enteric diseases

Yu, Jie; Langridge, William H. R.

doi:10.1038/89297

Cited by 224 publications

(119 citation statements)

References 26 publications

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“…To allow the efficient presentation of the P1 peptide to the mucosaassociated lymphoid tissue, while maintaining the structural integrity of the P1 peptide, we created a translational fusion between the C terminus of CTB and the peptide. The epithelialbinding subunits of bacterial enterotoxins such as CTB (41,42) and the homologous B subunit of the heat labile toxin of E. coli (31,34,43) are known to be potent mucosal immunogens, which can efficiently present fused antigenic determinants to the immune system. Based on the ability of CTB-P1, but not CTB, to bind GalCer in vitro (data not shown), and the aggregation of CTB-P1 oligomers into larger oligomeric structures (Figs.…”

Section: Discussionmentioning

confidence: 99%

A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs

Matoba

Magérus

Geyer

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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A vaccine that would engage the mucosal immune system against a broad range of HIV-1 subtypes and prevent epithelial transmission is highly desirable. Here we report fusing the mucosal targeting B subunit of cholera toxin to the conserved galactosylceramide-binding domain (including the ELDKWA-neutralizing epitope) of the HIV-1 gp41 envelope protein, which mediates the transcytosis of HIV-1 across the mucosal epithelia. Chimeric protein expressed in bacteria or plants assembled into oligomers that were capable of binding galactosyl-ceramide and G M1 gangliosides. Mucosal (intranasal) administration in mice of the purified chimeric protein followed by an i.p. boost resulted in transcytosis-neutralizing serum IgG and mucosal IgA responses and induced immunological memory. Plant production of mucosally targeted immunogens could be particularly useful for immunization programs in developing countries, where desirable product traits include low cost of manufacture, heat stability, and needle-free delivery.

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Section: Discussionmentioning

confidence: 99%

A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs

Matoba

Magérus

Geyer

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…As it is a potential mucosal and parenteral adjuvant and an effective carrier for chemically or genetically linked antigens, CT-B has often been expressed in the form of fusion proteins with antigens which do not induce sufficient immune response when orally applied on their own, much like LT-B. Examples of such use are CT-B-neutralizing epitope of the porcine epidemic diarrhea virus fusion protein (sCTB-sCOE) in lettuce (Huy et al, 2011), CT-Brabies glycoprotein fusion protein expressed in tobacco seeds (Tiwari et al, 2009), CT-B-Vibrio cholera accessory colonization factor subunit A (ACFA) fusion in tomato (Sharma et al, 2008), fusion of simian-human immunodeficiency virus regulatory sequence and CT-B , simian immunodeficiency virus Gag p27 capside protein-CT-B fusion (Kim et al, 2004a) and CT-B-anthrax lethal factor fusion in potato leaf , CT-B-human insulin B chain in tobacco (Li et al, 2006) and CT-B-rotavirus enterotoxin NSP4 fusion protein in potato tubers (Yu and Langridge, 2001). Expression levels of CT-B in most plant systems were 0.01 -1 % TSP, with the lowest yield of 0.04 % TSP in tomato leaf and fruit (Jani et al, 2002) and the highest yield of 4 % TSP in tobacco leaf (Daniell et al, 2001).…”

Section: Cholera Toxin Subunit Bmentioning

confidence: 99%

Production of vaccines for treatment of infectious diseases by transgenic plants

Ledl

Luthar

2016

AAS

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Since the first pathogen antigen was expressed in transgenic plants with the aim of producing edible vaccine in early 1990s, transgenic plants have become a well-established expression system for production of alternative vaccines against various human and animal infectious diseases. The main focus of plant expression systems in the last five years has been on improving expression of well-studied antigens such as porcine reproductive and respiratory syndrome (PRRSV), bovine viral diarrhea disease virus (BVDV), footh and mouth disease virus (FMDV), hepatitis B surface antigen (HBsAg), rabies G protein, rotavirus, Newcastle disease virus (NDV), Norwalk virus capsid protein (NVCP), avian influenza virus H5N1, Escherichia coli heat-labile enterotoxin subunit B (LT-B), cholera toxin B (CT-B), human immunodeficiency virus (HIV), artherosclerosis, ebola and anthrax. Significant increases in expression have been obtained using improved expression vectors, different plant species and transformation methods.Key words: transgenic plants; vaccine production; recombinant proteins; antibodies; infectious diseases; risk assessment IZVLEČEK ZDRAVLJENJE NALEZLJIVIH BOLEZNI S CEPIVI PRIDOBLJENIMI S TRANSGENIMI RASTLINAMIPridobivanje aktivnih sestavin za cepiva s transgenimi rastlinami se je začelo v devetdesetih letih prejšnjega stoletja. Od takrat se transgene rastline uveljavljajo kot alternativni ekspresijski sistem za sintezo aktivnih komponent za cepiva proti številnim humanim in živalskim nalezljivim boleznim. V zadnjih petih letih je bil glavni poudarek razvoja namenjen optimizaciji rastlinskih ekspresijskih sistemov za dobro proučene antigene, kot so virus prašičjega respiratornega in reproduktivnega sindroma, virus goveje virusne diareje, virus parkljevke, površinski antigen hepatitisa B, G protein virusa stekline, rotavirus, virus atipične kokošje kuge, plaščni protein Norwalk virusa, sev ptičje gripe H5N1, temperaturno nestabilna B podenota enterotoksina Escherischia coli, kolera toksin B, HIV, arterioskleroza, ebola in antraks. Uporaba optimiziranih transformacijskih metod in ekspresijskih vektorjev v kombinaciji s primerno izbrano rastlinsko vrsto je v večini naštetih primerov vodila do opaznega izboljšanja sinteze posameznih učinkovin.

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“…This confirmed that foreign proteins can be immunogenic when presented to the mucosal immune system via an orally delivered transgenic potato. A multicomponent plant-based vaccine comprised of cholera toxin complementary DNA fused to a rotavirus enterotoxin and enterotoxigenic E. coli fimbrae antigen genes was expressed in transgenic potatoes (Yu & Langrióge, 2001 Fig. 3.…”

Section: Support the Immune Systemmentioning

confidence: 99%

Nutrition-based health in animal production

Adams

2006

Nutr. Res. Rev.

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Events such as BSE, foot and mouth disease and avian influenza illustrate the importance of animal health on a global basis. The only practical solution to deal with such problems has usually been mass culling of millions of animals at great effort and expense. Serious consideration needs to be given to nutrition as a practical solution for health maintenance and disease avoidance of animals raised for food. Health or disease derives from a triad of interacting factors; diet–disease agent, diet–host and disease agent–host. Various nutrients and other bioactive feed ingredients, nutricines, directly influence health by inhibiting growth of pathogens or by modulating pathogen virulence. It is possible to transform plant-based feed ingredients to produce vaccines against important diseases and these could be fed directly to animals. Nutrients and nutricines contribute to three major factors important in the diet–host interaction; maintenance of gastrointestinal integrity, support of the immune system and the modulation of oxidative stress. Nutrition-based health is the next challenge in modern animal production and will be important to maintain economic viability and also to satisfy consumer demands in terms of food quality, safety and price. This must be accomplished largely through nutritional strategies making optimum use of both nutrients and nutricines.

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A plant-based multicomponent vaccine protects mice from enteric diseases

Cited by 224 publications

References 26 publications

A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs

A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs

Production of vaccines for treatment of infectious diseases by transgenic plants

Nutrition-based health in animal production

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