A pivotal role for Pim-1 kinase in esophageal squamous cell carcinoma involving cell apoptosis induced by reducing Akt phosphorylation

Li, Shenglei; Yu, Xiaofeng; Zhang, Hongyan; Wang, Yange; Wang, Xin; Liu, Hongtao; Chen, Kuisheng

doi:10.3892/or_00000947

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Western blotting was performed according to standard methods as described previously (18,19). Briefly, ESCC tissues and normal esophageal tissues were lysed in a sample buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl, 1% Triton X-100; 100 μg/ml PMSF) on ice for 30 min.…”

Section: In Situ Hybridization (Ish)mentioning

confidence: 99%

Overexpression of Tiam1 predicts poor prognosis in patients with esophageal squamous cell carcinoma

Liu

Shi²,

Liu³

et al. 2011

Oncol Rep

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Abstract. Accumulating evidence has demonstrated that Tcell lymphoma invasion and metastasis 1 (Tiam1) plays an important role in the occurrence and development of several different tumors; however, to date, little research has been done to verify the potential role of Tiam1 as a prognostic marker for esophageal squamous cell carcinoma (ESCC). In the present study, we examined the expression of Tiam1 in ESCC tissues by immunohistochemistry, in situ hybridization, semi-quantitative RT-PCR and Western blotting methods and investigated the correlation between Tiam1 levels and prognosis of patients with ESCC. Tiam1 exhibited high expression in ESCC tissues, whereas the normal esophageal tissues showed negative or weak Tiam1 expression. Additionally, Tiam1 mRNA and protein expression levels were both significantly correlated with histology grade, clinical staging and lymph node metastasis (all P<0.05), but not related to age and gender (both P>0.05). Further, ESCC patients with strong Tiam1 mRNA (P=0.000) and protein (P=0.000) expression had a poorer prognosis than those with weak expression. These findings demonstrate that Tiam1 may be used as molecular marker for predicting the prognosis of patients with ESCC.

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Section: In Situ Hybridization (Ish)mentioning

confidence: 99%

Overexpression of Tiam1 predicts poor prognosis in patients with esophageal squamous cell carcinoma

Liu

Shi²,

Liu³

et al. 2011

Oncol Rep

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“…Previous studies have suggested that PIM-1 is important for carcinogenesis and metastasis in numerous types of human cancer (6)(7)(8)(9)(10)(11)(12). The present study selected 4 pairs of ACC tissue and corresponding normal tissues and revealed that PIM-1 was overexpressed in ACC.…”

Section: Discussionmentioning

confidence: 94%

“…PIM-1, Pim-1 proto-oncogene, serine/threonine kinase (PIM-1) is upregulated in a number of cancer subtypes and the overexpression of PIM-1 is thought to be involved in cancer-specific apoptosis signaling pathways (6)(7)(8)(9)(10)(11)(12). Apoptosis is modulated by complex pathways that involve a series of apoptosis-associated proteins.…”

Section: Introductionmentioning

confidence: 99%

Expression of PIM‑1 in salivary gland adenoid cystic carcinoma: Association with tumor progression and patients' prognosis

Zhu

Hou

et al. 2017

Oncol Lett

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Abstract. Pim-1 proto-oncogene, serine/threonine kinase (PIM-1) phosphorylates a series of substrates to exert its oncogenic function in numerous malignancies. The present study investigated the clinical significance of the PIM-1 protein, apoptosis status and apoptosis-associated proteins, including forkhead box O3a (FOXO3a), B cell lymphoma-2 (BCL-2) and BCL-2-associted agonist of cell death (BAD), were investigated in salivary gland adenoid cystic carcinoma (ACC) tissues. PIM-1 expression levels in 4 pairs of ACC tissues and corresponding normal salivary gland tissues were determined by western blot analysis. PIM-1, FOXO3a, BAD and BCL-2 expression levels in 60 ACC tissues were evaluated by immunohistochemistry (IHC). A terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay was performed to detect the apoptosis status of ACC tissues. PIM-1 was revealed to be highly expressed in ACC tissues compared with adjacent normal tissues. IHC staining results demonstrated high expression ratios of PIM-1, FOXO3a, BCL-2 and BAD [33.33% (20/60), 51.67% (31/60), 51.67% (31/60) and 55% (33/60)], respectively, and significant correlations between the expression of PIM-1 and FOXO3a and BCL-2 (P<0.05). Apoptotic rates were significantly associated with PIM-1, FOXO3a, BCL-2 and BAD expression levels (P<0.05). PIM-1 expression levels were significantly associated with tumor size, lymph node involvement, nerve invasion, distant metastasis and weakly associated with tumor node metastasis stage. Kaplan-Meier survival curves revealed that PIM-1 expression level was significantly associated with disease-free survival of patients with ACC (P=0.009). Cox regression multivariate analysis results revealed that histotype, distant metastasis and apoptotic rate were independent prognosis factors for ACC. Assessment of PIM-1 may be useful in investigating the malignant behaviors of ACC and predicting the outcome of patients with ACC. IntroductionSalivary gland adenoid cystic carcinoma (ACC) accounts for ~10% of cases of epithelial salivary tumors and has a low 5-year survival rate (<20% in patients with highly metastatic tumors) (1,2). The development of ACC involves the interaction of oncogenes and tumor suppressor genes, similar to most other types of tumor (3). However, the precise mechanisms underlying ACC carcinogenesis remain to be elucidated (4,5).PIM kinases are oncogenic and are known to phosphorylate numerous substrates to exert their functions and have important roles in numerous malignancies. PIM-1, Pim-1 proto-oncogene, serine/threonine kinase (PIM-1) is upregulated in a number of cancer subtypes and the overexpression of PIM-1 is thought to be involved in cancer-specific apoptosis signaling pathways (6-12). Apoptosis is modulated by complex pathways that involve a series of apoptosis-associated proteins. As a substrate of PIM-1 kinase, Forkhead box O3a (FOXO3a) is a proapototic transcription factor and regulates the expression of numerous apoptosis-associated genes to induce apoptosis (13). It has previously ...

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“…High expression of the proto-oncogene PIM-1 was detected in ESCC with lymph node metastasis[ 44 ], and PIM-1 siRNA inhibited the proliferation of ESCC cells and induced apoptosis[ 45 ]. Upregulation of PIM-1 was also found in gastric glands correlated with the lymph node metastasis of gastric cancer[ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Prediction of lymph node metastasis in early esophageal cancer

Li,

Wang,

Yibi

2023

World J Gastrointest Surg

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Postcholecystectomy bile duct injury (BDI) remains a devastating iatrogenic complication that adversely impacts the quality of life with high healthcare costs. Despite a decrease in the incidence of laparoscopic cholecystectomy-related BDI, the absolute number remains high as cholecystectomy is a commonly performed surgical procedure. Open Roux-en-Y hepaticojejunostomy with meticulous surgical technique remains the gold standard surgical procedure with excellent long-term results in most patients. As with many hepatobiliary disorders, a minimally invasive approach has been recently explored to minimize access-related complications and improve postoperative recovery. Since patients with gallstone disease are often admitted for a minimally invasive cholecystectomy, laparoscopic and robotic approaches for repairing postcholecystectomy biliary stricture are attractive. While recent series have shown the feasibility and safety of minimally invasive post-cholecystectomy biliary stricture management, most are retrospective analyses with small sample sizes. Also, long-term follow-up is available only in a limited number of studies. The principles and technique of minimally invasive repair resemble open repair except for the extent of adhesiolysis and the suturing technique with continuous sutures commonly used in minimally invasive approaches. The robotic approach overcomes key limitations of laparoscopic surgery and has the potential to become the preferred minimally invasive approach for the repair of postcholecystectomy biliary stricture. Despite increasing use, lack of prospective studies and selection bias with available evidence precludes definitive conclusions regarding minimally invasive surgery for managing postcholecystectomy biliary stricture. High-volume prospective studies are required to confirm the initial promising outcomes with minimally invasive surgery.

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A pivotal role for Pim-1 kinase in esophageal squamous cell carcinoma involving cell apoptosis induced by reducing Akt phosphorylation

Cited by 8 publications

References 24 publications

Overexpression of Tiam1 predicts poor prognosis in patients with esophageal squamous cell carcinoma

Overexpression of Tiam1 predicts poor prognosis in patients with esophageal squamous cell carcinoma

Expression of PIM‑1 in salivary gland adenoid cystic carcinoma: Association with tumor progression and patients' prognosis

Prediction of lymph node metastasis in early esophageal cancer

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