A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens

Cortes, Marcio Vinicius de Carvalho Barros; Oliveira, Maythsulene Inácio de Sousa; Mateus, Jackeline Rossetti; Seldin, Lucy; Silva-Lobo, V. L.; Freire, Denise Maria Guimarães

doi:10.1016/j.biocontrol.2020.104460

Cited by 10 publications

(2 citation statements)

References 48 publications

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“…Among them, S. oryzae, S. attenuatum and S. sinense were reported as pathogen of rice (Giraldo et al 2015, Prasannakumar et al 2021). However, S. oryzae could synthesis cerulenin ((2R, 3S, E)-2,3-Epoxy-4-oxo-7,10-dodecadienamide), which was a potent antifungal agent and could be used as biocontrol agents (Côrtes et al 2021). The result of Côrtes et al (2021) showed that the genetic improved S. oryzae had a good control effect on root rot and white mold disease (Côrtes et al 2021).…”

Section: Resultsmentioning

confidence: 99%

“…However, S. oryzae could synthesis cerulenin ((2R, 3S, E)-2,3-Epoxy-4-oxo-7,10-dodecadienamide), which was a potent antifungal agent and could be used as biocontrol agents (Côrtes et al 2021). The result of Côrtes et al (2021) showed that the genetic improved S. oryzae had a good control effect on root rot and white mold disease (Côrtes et al 2021). The role of the genus Sarocladium in wheat soil needs to be further studied, so that relevant measures could be taken in the early stage of wheat planting, in order to better provide basis for subsequent wheat planting.…”

Section: Resultsmentioning

confidence: 99%

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Effect of fertilisation on fungal community in topsoil of winter wheat field

Zhai¹,

Li²,

Qin³

et al. 2022

PLANT SOIL ENVIRON

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Soil fungi played important roles in the maintenance of soil fertility and soil sustainable development. In this study, the effects of different fertilisers (i.e. bacterial fertiliser (BF), composed of organic matters and bacteria; mineral fertiliser (MF), composed of N, P and K) on soil fungi in wheat field were analysed. The results showed that the yield of winter wheat with BF was 4 788.52 kg/ha, which was significantly higher than that with term MF. Chao 1<br />and Shannon indexes and principal coordinates analysis showed that fertilisation increased the richness of soil fungi to varying degrees and changed the fungal community structure of soil compared with no fertiliser control (NF). The soil fungal community was mainly composed of Ascomycota, Basidiomycota and Mortierellomycota, with Ascomycota as the main species (62.67–65.08%). Compared with MF, the relative abundance of potential beneficial fungi Talaromyces in BF increased 4.44 times. Compared with no fertiliser control, the relative abundance of potential beneficial fungi Chrysosporium in BF increased 4.11 times. The abundance of potential soil pathogenic fungi (P < 0.01), like Stachybotrys, Acrocalymma, Achroiostachys, Arachnomyces and Setophoma, significantly decreased in BF treatment, which was beneficial to the maintenance of crop health and the sustainable development of the environment. Moreover, the network analysis showed that the interspecific relationship of soil fungi in BF was more intimate than MF and NF and fungi were inclined to adopt cooperative manner to adapt ecological niches in BF treatment. The improvement of wheat yield might be due to the optimisation of soil fungal community structure by applying BF, which strengthened the transformation of nutrients in soil, increased some biocontrol microorganism, and reduced the crop disease. The results explain the improvement of wheat yield by BF to a certain extent, and provided theoretical basis for high-yield cultivation of wheat.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of fertilisation on fungal community in topsoil of winter wheat field

Zhai¹,

Li²,

Qin³

et al. 2022

PLANT SOIL ENVIRON

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An avirulent Ralstonia solanacearum strain FJAT1458 outcompetes with virulent strain and induces tomato plant resistance against bacterial wilt

Zheng

Xiao

Chen

et al. 2022

Pest Management Science

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BACKGROUND Bacterial wilt (BW) caused by Ralstonia solanacearum (RS) is considered as one of the most destructive plant diseases. An avirulent strain of RS, FJAT1458, is a potential biocontrol agent of BW. In this study, the mechanism of FJAT1458 against BW was evaluated. RESULTS FJAT1458 was tagged with the red fluorescent protein gene, and the resulting strain was named as FJAT1458‐RFP. When FJAT1458‐RFP and FJAT91‐GFP (a virulent strain of RS labelled with the green fluorescent protein gene), were co‐inoculated in potted tomato plants, the colonization of FJAT91‐GFP reached an almost undetectable level at 7 days post‐inoculation (dpi) in the roots and at 9 dpi in rhizosphere soil. When they were co‐inoculated in a hydroponic tomato growing system, numbers of the two strains were similar at 3 dpi in the root tissues; however, FJAT91‐GFP was not detected at 9 dpi while FJAT1458‐RFP maintained 1.77 × 105 CFU g−1. The inoculation of FJAT1458‐RFP alone or combination with FJAT91‐GFP significantly increased tomato root activity. Moreover, expression levels of the defense‐related genes PR‐1a, GLUA, and CHI3 in tomato roots were significantly up‐regulated by FJAT1458‐RFP and co‐inoculation of FJAT1458‐RFP and FJAT91‐GFP at 5 dpi, compared to the control (water, CK) treatment. Noteworthy, expression levels of GLUA in the treatments of FJAT1458‐RFP and FJAT1458‐RFP + FJAT91‐GFP were 12.22‐ and 12.05‐fold higher than that in the CK at 5 dpi, respectively. CONCLUSIONS The results suggested that the avirulent strain FJAT1458‐RFP could suppress colonization of the virulent strain in tomato roots, and induce tomato plant resistance against BW. © 2022 Society of Chemical Industry.

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