A pipeline for making 31P NMR accessible for small- and large-scale lipidomics studies

Furse, Samuel; Williams, Huw E. L.; Watkins, Adam; Virtue, Samuel; Vidal-Puig, António; Amarsi, Risha; Charalambous, Marika; Koulman, Albert

doi:10.1007/s00216-021-03430-4

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…Mass spectrometry samples were prepared and data collected in a high throughput fashion using Direct Infusion Mass Spectrometry (Harshfield et al, 2019 ), via glass-coated 384 well plates. NMR samples were prepared and data collected in a low throughput fashion using a modified (Furse et al, 2020a ) form of the CUBO solvent system (Bosco et al, 1997 ; Cremonini et al, 2004 ; Culeddu et al, 1998 ; Murgia et al, 2003 ) and assigned using reference 2D spectra acquired for the purpose (Furse et al, 2020a , 2021c ). Lipidomics data were interpreted using dual spectroscopy (Furse et al, 2020a ), in the case of these data, a combination of DIMS and 31 P NMR was used to establish the composition of phospholipids.…”

Section: Methodsmentioning

confidence: 99%

Paternal nutritional programming of lipid metabolism is propagated through sperm and seminal plasma

et al. 2022

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Background The paternal diet affects lipid metabolism in offspring for at least two generations through nutritional programming. However, we do not know how this is propagated to the offspring. Objectives We tested the hypothesis that the changes in lipid metabolism that are driven by paternal diet are propagated through spermatozoa and not seminal plasma. Methods We applied an updated, purpose-built computational network analysis tool to characterise control of lipid metabolism systemically (Lipid Traffic Analysis v2.3) on a known mouse model of paternal nutritional programming. Results The analysis showed that the two possible routes for programming effects, the sperm (genes) and seminal plasma (influence on the uterine environment), both have a distinct effect on the offspring’s lipid metabolism. Further, the programming effects in offspring suggest that changes in lipid distribution are more important than alterations in lipid biosynthesis. Conclusions These results show how the uterine environment and genes both affect lipid metabolism in offspring, enhancing our understanding of the link between parental diet and metabolism in offspring.

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Section: Methodsmentioning

confidence: 99%

Paternal nutritional programming of lipid metabolism is propagated through sperm and seminal plasma

et al. 2022

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“…Recently, a simple method to quantify LysoPLs in food emulsions by 31P NMR, a sensitive and precise method of quantification of LysoPLs has been suggested [ 179 ]. Moreover, in lipidomics, NMR has been mostly combined with mass spectrometry approaches to reach a complete and robust PL profile [ 180 – 183 ].…”

Section: Phospholipidomics Approaches In Clinical Trialsmentioning

confidence: 99%

Phospholipidomics in Clinical Trials for Brain Disorders: Advancing our Understanding and Therapeutic Potentials

Hachem,

Ahmmed,

Nacir-Delord

2023

Mol Neurobiol

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Phospholipidomics is a specialized branch of lipidomics that focuses on the characterization and quantification of phospholipids. By using sensitive analytical techniques, phospholipidomics enables researchers to better understand the metabolism and activities of phospholipids in brain disorders such as Alzheimer’s and Parkinson’s diseases. In the brain, identifying specific phospholipid biomarkers can offer valuable insights into the underlying molecular features and biochemistry of these diseases through a variety of sensitive analytical techniques. Phospholipidomics has emerged as a promising tool in clinical studies, with immense potential to advance our knowledge of neurological diseases and enhance diagnosis and treatment options for patients. In the present review paper, we discussed numerous applications of phospholipidomics tools in clinical studies, with a particular focus on the neurological field. By exploring phospholipids’ functions in neurological diseases and the potential of phospholipidomics in clinical research, we provided valuable insights that could aid researchers and clinicians in harnessing the full prospective of this innovative practice and improve patient outcomes by providing more potent treatments for neurological diseases. Graphical Abstract

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“…Dual spectroscopy [71] was used to interpret and finalise the lipidomic data. Specifically: the 31 P NMR data of brain and heart tissue were collected and assigned (according to [72][73][74][75]) and used to determine the difference in ionisation efficiency between species.…”

Section: Offspring Tissue Lipidome Profilingmentioning

confidence: 99%

Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice

Furse

Morgan

Koulman

et al. 2023

IJMS

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There is growing evidence that poor paternal diet at the time of conception increase the risk of offspring developing a range of non-communicable metabolic diseases, such as obesity, diabetes and cardiovascular disease, in adulthood. We hypothesise that a paternal low protein–high carbohydrate diet perturbs offspring tissue lipid abundance through both sperm and seminal plasma-mediated mechanisms. To test our hypothesis, we fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. We generated offspring through artificial insemination, in combination with vasectomised male mating. Using this approach, we derived offspring from either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Using high resolution mass-spectrometry, we found that offspring derived from either LPD sperm or seminal fluid displayed perturbed cardiac and brain lipid abundance from just three weeks of age, typically associated with the altered abundance of tissue triglycerides. We also observed the differential sex-specific patterns of lipids between the control and experimental offspring’s hearts and brains. These observations indicate that poor paternal diet at the time of conception affects offspring cardiac and brain lipid profiles in an age-, sex- and generation-specific manner.

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A pipeline for making 31P NMR accessible for small- and large-scale lipidomics studies

Cited by 12 publications

References 44 publications

Paternal nutritional programming of lipid metabolism is propagated through sperm and seminal plasma

Paternal nutritional programming of lipid metabolism is propagated through sperm and seminal plasma

Phospholipidomics in Clinical Trials for Brain Disorders: Advancing our Understanding and Therapeutic Potentials

Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice

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