A Pilot Study on the Effect of Cannabis Extract on Weight Loss in Diet-induced Obese Mice

Abstract

Objectives This study aimed to investigate the pharmacological effect of cannabis extract on weight loss in diet-induced obese mice. Methods A total of 12 C57BL/6 male mice (Orient Bio), aged 6 weeks, were fed a high-fat diet for 13 weeks to construct a diet-induced obesity model. During the following 5 weeks, diet-induced obese mice were daily administered cannabis extract or sesame seed oil orally along with the high-fat diet. The body weight of each subject was measured weekly. Venous blood was drawn for biochemistry, enzyme-linked immunoassay, and oral glucose tolerance test before and after treatment. Body fat was measured by dual-energy X-ray absorptiometry, and the mesenteric adipose tissue was also measured after sacrifice. We used exact Wilcoxon's two-sample analyses and generalized estimating equations to test the differences between the cannabis-treated group and control. Results There was significant weight loss (p=0.009) observed in the cannabis-treated mice compared to the control group after 5 weeks of treatment. High-fat diet-induced glucose intolerance in the cannabis-treated group was significantly ameliorated (p=0.032), whereas there were no profound differences between the two groups in terms of other physiological markers, including corticosterone level. Conclusion This study shows that orally administered cannabis extract had a pharmacological effect of weight loss in diet-induced obese mice. This weight loss might be attributed to an increase in energy expenditure and regulation of glucose homeostasis.

Use of N-Acetylcysteine for Clozapine-Induced Acute Liver Injury: A Case Report and Literature Review

“…8,18 The mechanism of clozapine-induced DILI is unknown and, however, may be caused by the production of a clozapine toxic metabolite. 19 The use of NAC in the early treatment of acetaminopheninduced ALF is well established. 20,21 For nonacetaminophen-induced ALF, beneficial effects of NAC have been reported in observational studies and at least 2 clinical trials.…”

“…8,18 The mechanism of clozapine-induced DILI is unknown and, however, may be caused by the production of a clozapine toxic metabolite. 19…”