A Pilot Study on the Immunomodulatory Effect of Bulgarian Propolis

Abstract

Propolis is a product from the honey bee A. mellifera with various pharmacological properties. Its immunomodulatory activity is in the focus of T helper/inducer (CD4+CD3+), T cytotoxic (CD8+CD3+), B (CD19+CD3-) and NK (CD56+CD16+CD3-) lymphocyte subsets, as well as the proportion of apoptotic (Annexin V+) cells within each subset were determined before and after the cultivation by flow cytometry (FACS Calibur, BD). The percentage of CD19+ cells decreased in high concentrations of both of substances, but in low concentrations they had a protective effect on the proliferation and B cell activity. Low dozes had no effect on the percentage of CD4

The antiviral and immunomodulatory activities of propolis: An update and future perspectives for respiratory diseases

“…Low concentrations of propolis (1–2.5 µg/ml) had a protective effect on the activity and proliferation of B cells but did not affect the percentage of CD4 + and CD8 + T‐cells. No negative effects could be seen on the proliferation and vitality of NK cells 153 …”

“…NO generation was inhibited in a concentration-dependent manner by both propolis and flavonoids. 152 Draganova-Filipova et al 153 evaluated Bulgarian propolis ethanolic extract immunomodulatory activity on PBMCs, assessing the percentage of T helper/inducer (CD4 + CD3 + ), T cytotoxic (CD8 + CD3 + ), B (CD19 + CD3 − ), and NK (CD56 + CD16 + CD3 − ) lymphocyte subsets, as well as the proportion of apoptotic (annexin V + ) cells within each subset. Low concentrations of propolis (1-2.5 µg/ml) had a protective effect on the activity and proliferation of B cells but did not affect the percentage of CD4 + and CD8 + T-cells.…”

“…No negative effects could be seen on the proliferation and vitality of NK cells. 153 Sampietro et al 154 assessed the immunomodulatory activity of delipidated extracts of propolis from the North of Argentina (mainly derived from Salix humboldtiana Willd., Pinus spp., and Eucalyptus spp. ), and purified galangin and pinocembrin.…”

Literature Review: Potensi Propolis Sebagai Imunomodulator

“…Kandungan flavonoid dalam propolis bervariasi sekitar 10-20%. Kandungan tersebut merupakan yang terbanyak dibandingkan kandungan flavonoid dalam produk lebah lain (Draganova-Filipova, et al, 2010;Sforcin, 2007;Sforcin & Bankova, 2011). Di Indonesia lebah Trigona Sp merupakan penghasil propolis berkualitas tinggi yang mengandung banyak flavonoid (Hasan, et al, 2011;Hasan, et al, 2013;Syamsudin, et al, 2009).…”

Bulgarian Bee Products and their Health Promoting Potential

“…Helicobacter pylory (10,11) and Salmonella (36); anti-influenza virus activity (29,43), antiparasitic activity against Tripanozoma cruzii (12,40) and Leishmania (28). the immunomodulatory activity of Bulgarian propolis has also been reported (13,14,15). the antioxidant potential of Bulgarian propolis was studied and it was found that different flavonoids and phenolic acid derivatives contribute to these properties (31,48).…”