A pilot study on bacterial viability in acne. Assessment using dual flow cytometry on microbials present in follicular casts and comedones

Arrese, Jorge E.; Goffin, Veroniqué; Avila‐Camacho, Mabel; Greimers, Roland; Pierard, Gérald

doi:10.1046/j.1365-4362.1998.00468.x

Cited by 7 publications

(13 citation statements)

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“…Such a technique can be applied to bacteria present in follicular casts harvested using cyanoacrylate skin surface strippings [7]. A preliminary pilot study had suggested the superiority of minocycline over lymecycline in eradicating intrafollicular bacteria [7]. Indirect evidence for reduced risk of induction of bacterial resistance was also shown for minocycline, thus confirming previous observations [1].…”

Section: Introductionsupporting

confidence: 72%

“…Both drugs are available for many years and have clearly demonstrated a good profile of efficacy combined with a global low risk of side effects [1][2][3][4][5]. Studies comparing the efficacy of these antibiotics at different dosages are rare [6,7]. In addition, the relationship between clinical efficacy and in situ antibacterial activity has been seldom evaluated [2,7].…”

Section: Introductionmentioning

confidence: 99%

“…Studies comparing the efficacy of these antibiotics at different dosages are rare [6,7]. In addition, the relationship between clinical efficacy and in situ antibacterial activity has been seldom evaluated [2,7]. In particular, the influence of the relative proportion in live and dead bacteria on inflammation is unknown.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years new fluorescent probes have been made available to study the viability of microorganisms using dual-flow cytometry [5,[7][8][9]. Such a technique can be applied to bacteria present in follicular casts harvested using cyanoacrylate skin surface strippings [7].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lymecycline and Minocycline in Inflammatory Acne

Piérard-Franchimont

Goffin

Arrese

et al. 2002

Skin Pharmacol Physiol

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Background: Some antibiotics represent a mainstay in acne treatment. However, studies comparing their efficacies are rare. Aim: To evaluate the clinical and in vivo antibacterial effect of lymecycline and minocycline at different dosages. Method: Eighty-six patients with moderate to severe acne were enrolled in a randomized, double-blind, intent-to-treat study comparing in three parallel groups the effect of (1) lymecycline 300 mg daily for 12 weeks, (2) minocycline 50 mg daily for 12 weeks and (3) minocycline 100 mg daily for 4 weeks followed by 50 mg daily for 8 weeks. Evaluations were made at the screening visit and at five on-treatment visits. They consisted of clinical counts of acne lesions and evaluations of bacterial viability using dual flow cytometry performed on microorganisms collected from sebaceous infundibula by cyanoacrylate strippings. Results: Patients receiving minocycline 100/50 mg had the best clinical outcome, particularly in the reduction of the number of papules. By the end of the trial, the microbial response to minocycline 100/ 50 mg was also superior to either of the other two treatments. There were less live and more dead bacteria. Conclusion: In this trial, minocycline 100/50 mg was superior for the treatment of inflammatory acne when compared to lymecycline 300 mg and minocycline 50 mg.

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Section: Introductionsupporting

confidence: 72%