A pilot study of LINE-1 copy number and telomere length with aging in human sperm

Berteli, Thalita S.; Wang, Fang; Navarro, Paula Andrea; Kohlrausch, Fabiana B.; Keefe, David L.

doi:10.1007/s10815-023-02857-1

Cited by 3 publications

(1 citation statement)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with these findings, a recently published study observed that, in contrast to other cells, telomere length in spermatozoa increases with age, and this is associated with a reduction in LINE1 gene expression. However, the study did not analyze the methylation rate of LINE1 , so we cannot be certain that telomere lengthening is responsible for hypermethylation and thus repression of gene expression [ 43 ]. Finally, it has recently been shown that age-related paternal hypermethylation of LINE1 can be transmitted to offspring.…”

Section: Discussionmentioning

confidence: 99%

Relationship between degree of methylation of sperm long interspersed nuclear element-1 (LINE-1) gene and alteration of sperm parameters and age: a meta-regression analysis

Crafa,

Leanza,

Condorelli

et al. 2023

J Assist Reprod Genet

View full text Add to dashboard Cite

Introduction The long interspersed nuclear element-1 (LINE1) gene is a retrotransposon whose methylation status appears to play a role in spermatogenesis, the outcome of assisted reproductive techniques (ART), and even in recurrent pregnancy loss (RPL). Advanced paternal age appears associated with altered sperm parameters, RPL, poor ART outcomes, and compromised offspring health. The methylation status of LINE1 has been reported to be affected by age. The latest meta-analysis on the LINE1 methylation pattern in spermatozoa found no significant differences in methylation levels between infertile patients and fertile controls. However, to the best of our knowledge, no updated meta-analysis on this topic has been published recently. Furthermore, no comprehensive meta-regression analysis was performed to investigate the association between sperm LINE1 methylation pattern and age. Objectives To provide an updated and comprehensive systematic review and meta-analysis on sperm LINE1 gene methylation degree in patients with abnormal sperm parameters compared to men with normal sperm parameters and to probe the association between sperm LINE1 methylation status and age and/or sperm concentration. Methods This meta-analysis was registered in PROSPERO (registration n. CRD42023397056). It was performed according to the MOOSE guidelines for Meta-analyses and Systematic Reviews of Observational Studies and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P). Only original articles evaluating LINE1 gene methylation in spermatozoa from patients with infertility or abnormalities in one or more sperm parameters compared to fertile or normozoospermic men were included. Results Of 192 abstracts evaluated for eligibility, only 5 studies were included in the quantitative synthesis, involving a total of 340 patients and 150 controls. Our analysis showed no significant difference in LINE1 gene methylation degree in patients with infertility and/or abnormal sperm parameters compared to fertile controls and/or men with normal sperm parameters, although there was significant heterogeneity across studies. No significant evidence of publication bias was found, and no study was sensitive enough to alter the results. In meta-regression analysis, we found that the results were independent of both ages and sperm concentration. A sub-analysis examining patients and controls separately was also conducted and we found a trend for a positive correlation between LINE1 methylation and sperm concentration in the control group only. Conclusions The results of this systematic review and meta-analysis do not suggest a determining role of sperm LINE1 gene methylation degree in patients with infertility and/or abnormal sperm parameters. Therefore, we do not suggest including LINE1 in the genetic panel of prospective studies aimed at identifying the most representative and cost-effective genes to be analyzed in couples undergoing ART cycles.

show abstract

Section: Discussionmentioning

confidence: 99%

Relationship between degree of methylation of sperm long interspersed nuclear element-1 (LINE-1) gene and alteration of sperm parameters and age: a meta-regression analysis

Crafa,

Leanza,

Condorelli

et al. 2023

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

LINE-1 transposable element renaissance in aging and age-related diseases

Li,

Yu,

et al. 2024

Ageing Research Reviews

View full text Add to dashboard Cite

Telomeric function and regulation during male meiosis in mice and humans

Yin,

Jiang,

et al. 2024

Andrology

View full text Add to dashboard Cite

BackgroundTelomeres are unique structures situated at the ends of chromosomes. Preserving the structure and function of telomeres is essential for maintaining genomic stability and promoting genetic diversity during male meiosis in mammals.Material‐MethodsThis review compiled recent literature on the function and regulation of telomeres during male meiosis in both mice and humans, and also highlighted the critical roles of telomeres in reproductive biology and medicine.Results‐DiscussionVarious structures, consisting of the LINC complex (SUN‐KASH), SPDYA‐CDK2, TTM trimer (TERB1‐TERB2‐MAJIN), and shelterin, are critical in controlling telomeric activities, such as nuclear envelope attachment and bouquet formation. Other than telomere‐related proteins, cohesins and genes responsible for regulating telomere function are also highlighted, though the exact mechanism remains unclear. The gene‐mutant mouse models with meiotic defects directly reveal the essential roles of telomeres in male meiosis. Recently reported mutant genes associated with telomere activity in clinical practice have also been illustrated in detail.ConclusionsProper regulation of telomere activities is essential for male meiosis progression in mice and humans.

show abstract

A pilot study of LINE-1 copy number and telomere length with aging in human sperm

Cited by 3 publications

References 74 publications

Relationship between degree of methylation of sperm long interspersed nuclear element-1 (LINE-1) gene and alteration of sperm parameters and age: a meta-regression analysis

Relationship between degree of methylation of sperm long interspersed nuclear element-1 (LINE-1) gene and alteration of sperm parameters and age: a meta-regression analysis

LINE-1 transposable element renaissance in aging and age-related diseases

Telomeric function and regulation during male meiosis in mice and humans

Contact Info

Product

Resources

About