A pilot study of Helicobacter pylori genotypes and cytokine gene polymorphisms in reflux oesophagitis and peptic ulcer disease

Abstract

Abstract:Helicobacter pylori causes various diseases such as chronic gastritis, peptic ulcer and gastric cancer. While majority of the people infected with H. pylori is asymptomatic, 15-20 % of them develop such diseases. The main factors, which determine the development of H. pylori related diseases might be bacterial virulence, host genetic and environmental factors. The aim of this study was to reveal the factors that play a role in the disease development in patients with refl ux esophagitis and peptic ulcer, infected with Helicobacter pylori. Environmental factors such as medical agents, smoking and body mass index were evaluated. The factors specifi c to bacteria such as vacA, CagA, babA and iceA virulence genotypes and the host factors such as IL-1, IL-2, IL-4, IL-6, IL-10, IL-12, interferon-γ, TNF-α, ve TGF-β 1 gene polymorphisms were compared between the two groups. H. pylori infected twenty fi ve patients with refl ux esophagitis and peptic ulcer were enrolled in the study. There was no statistical difference between the two groups regarding environmental factors. IL-2 -330T +166T (p=0.037) and IL10 -1082A; -819C (p=0.049) gene polymorphisms were signifi cantly more common in the group of patients with peptic ulcer compared to the group with refl ux esophagitis. In both groups of patients, either with refl ux esophagitis or peptic ulcer, multiple H. pylori virulence genotypes (cagA, vacA, babA) (mean values 74 %, 78 %, 54 % respectively) were observed. In this study, we revealed that cytokine gene polymorphisms may play a role in the development peptic ulcer while H. pylori virulence genotypes seem to be crucial for the development of associated diseases (Tab. 4, Ref. 51). Text in PDF www.elis.sk.

Circulating Levels of Adipocytokines as Potential Biomarkers for Early Detection of Colorectal Carcinoma in Egyptian Patients

“…Colon cancer comes third among the most common types of cancer (MJ 2002;Akdogan et al, 2014). Adipocytokines produced by adipose tissue have been investigated as new risk factors for cancer and metabolic syndromes (Nakajima et al, 2010;Akdogan et al, 2014).…”

“…Adipocytokines produced by adipose tissue have been investigated as new risk factors for cancer and metabolic syndromes (Nakajima et al, 2010;Akdogan et al, 2014).…”

“…During the last two decades, fat tissue has become gradually observed not only as an energy storage depot but also as an active endocrine organ that produces and secretes proteins acting as hormones called adipocytokines or adipohormones (Kumor et al, 2009;Akdogan et al, 2014).…”

Genes de patogenicidade de Helicobacter pylori, polimorfismos de citocinas e fatores ambientais afetam o desenvolvimento de doenças gástricas: uma visão geral

“…73 It is capable of inhibiting the production of pro-inflammatory cytokines like IFN-γ, IL-1, IL-2, IL-3, IL-6, IL-8, TNF-α, and GM-CSF and inducing B-cell proliferation and differentiation. 74,75 A C-T base transition located at positions -819, and C-A base transition at position -592 have been identified in the 5´ flanking region of the IL-10 gene. These SNPs are related to different serum level of IL-10 in vivo.…”

Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis

“…H. pylori infection has been associated with a variety of diseases, such as chronic gastritis, peptic ulcer and gastric carcinoma. However, the majority of people infected with H. pylori are asymptomatic, and only 15%-20% of them develop such gastroduodenal diseases [2]. It has been hypothesized that a combination of environmental/behavioral variables, host genetic factors and bacterial pathogenicity genes determine the clinical outcome of H. pylori infection and the severity of gastric damage [3].…”